A5083477967- Force Microscopy Techniques and Applications
- Mechanical and Optical Resonators
- Advanced biosensing and bioanalysis techniques
- Near-Field Optical Microscopy
- Adhesion, Friction, and Surface Interactions
- SARS-CoV-2 detection and testing
- Molecular Junctions and Nanostructures
- Biosensors and Analytical Detection
- Carbon and Quantum Dots Applications
- Surface Modification and Superhydrophobicity
- Nanoparticle-Based Drug Delivery
- Electronic and Structural Properties of Oxides
- Diamond and Carbon-based Materials Research
- Nanofabrication and Lithography Techniques
- Gas Sensing Nanomaterials and Sensors
- Graphene research and applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Microplastics and Plastic Pollution
- nanoparticles nucleation surface interactions
- Characterization and Applications of Magnetic Nanoparticles
- Chalcogenide Semiconductor Thin Films
- Surface and Thin Film Phenomena
- Lubricants and Their Additives
- Advanced Electron Microscopy Techniques and Applications
- Epilepsy research and treatment
Universidad Autónoma de Madrid
1999-2025
Institute of Micro and Nanotechnology
2020-2025
Consejo Superior de Investigaciones Científicas
2006-2022
National Changhua University of Education
2022
Unidades Centrales Científico-Técnicas
2022
Centro Nacional de Microelectrónica
2010-2015
Instituto de Investigaciones Químicas
2008
CIC biomaGUNE
2008
Luna Innovations (United States)
2008
Lawrence Berkeley National Laboratory
1998-2005
In this work, we present the combination of two different types nanomaterials, 2D molybdenum disulfide nanosheets (MoS2-NS) and zero-dimensional carbon nanodots (CDs), for development a new electrochemiluminescence (ECL) platform early detection quantification biomarker human epidermal growth factor receptor 2 (HER2), whose overexpression is associated with breast cancer. MoS2-NS are used as an immobilization thiolated aptamer, which can recognize HER2 epitope peptide high affinity, CDs act...
The adsorption of water on several alkali halide surfaces was studied using scanning polarization force microscopy. Water leads to an overall increase surface potential and ionic mobility. At a critical humidity that is characteristic each salt (NaCl, KCl, KBr, KI), important changes in the rate mobility occurred. Topographical occurred as well, form step motion. These were observed occur at fast above point, while little motion lower humidity. Dissolution crystal (deliquescence) high Below...
Abstract Gold nanotriangles (AuNTs) functionalized with dithiolated oligonucleotides have been employed to develop an amplification-free electrochemical biosensor for SARS-CoV-2 in patient samples. nanotriangles, prepared through a seed-mediated growth method and exhaustively characterized by different techniques, serve as improved platform DNA probe immobilization. Azure A is used indicator of the hybridization event. The detects either single stranded or RNA sequences lengths, low...
In this work, an electrochemical biosensor is prepared based on few-layer bismuthene hexagons (FLBHs) and a water-soluble BODIPY (BDP) derivative (BDP-NaSO3) for early infection diagnosis. particular, the detection in advance of virus sequence nasopharyngeal swab samples was developed. The combination FLBHs BDP-NaSO3 facilitates direct, sensitive, specific gene viruses without need any prior amplification step. This work demonstrates that provide improved platform immobilizing thiolated DNA...
Abstract An electrochemical DNA biosensor is presented for early viral infection detection, integrating molybdenum disulphide (MoS₂), tetrahedral nanostructures (TDNs), and thionine-modified carbon nanodots (CNDsTy). The innovation of this work lies in the first-time integration these nanomaterials preparation a bioconjugate, whose synergy enables biosensor’s functionality. MoS₂ anchors TDNs, which carry capture probe virus identification via genetic code recognition. CNDsTy allow detection...
The scope of the noncontact scanning force microscopy technique concerning analysis fragile and weakly attached samples is shown by proving its ability to investigate water−graphite interface. After a macroscopic quantity purified water has been shacked out from graphite surface, image taken in air at relative humidity value 60% reveals nanodroplets steps. In high atmosphere (>90%), adsorbs on surface forming flat rounded islands 5 nm height that transform 2 when stabilizes 90%. This process...
Abstract The sticking effect between hydrophilic surfaces occurring at increasing relative humidity (RH) is an everyday phenomenon with uncountable implications. Here experimental evidence presented for a counterintuitive monotonous decrease of the capillary adhesion forces RH whole range. It shown that this unexpected result related to actual shape asperity nanometer scale: model based on macroscopic thermodynamics predicts in force sharp object ending almost flat nanometer‐sized apex, full...
ZnO films have been grown by pulsed electrochemical deposition between a reduction potential and an oxidation to improve their quality. Different morphologies like columns, platelets, flowers, high-quality planar are obtained changing the without further addition of additives. The mechanism behind this control in morphology is ascribed OH– concentration at electrode surface (pH) as consequence potential. morphology, potential, electrical conduction were simultaneously measured kelvin probe...
A method for measuring friction forces on a nanometer scale is described. This combines lock-in technique with scanning force and microscopy. Essentially, amplifier used to determine the amplitude of loop, which measured at high frequency. To demonstrate capability this method, dependence normal load two dimensional image presented.
Scanning polarization force microscopy was used to study the topography, polarizability, and contact potential of cleaved NaCl(100) as a function relative humidity (RH) between < 5% 40%. In this range there are reversible changes in surface while large scale modifications step topography irreversible ion redistribution occur above 40% RH. dry conditions more negative near atomic steps than over flat terraces. As increased, were observed local polarizability due ionic solvation, terraces...
We have studied the intermittent contact mode for small oscillation amplitudes and soft cantilevers with sharp tips. For appropriate working conditions tip does not touch mica surface. Instead, a high dissipative tip–sample interaction takes place which reduces amplitude of cantilever. Our experiments show that this is related to relative humidity we believe it induced by nanometer sized liquid neck between sample. A phase contrast image on different surface materials then due mainly wetting...
Tip−sample interaction of an oscillating tip near a surface is determined. The experimental results show that the presence can be detected without mechanically touching surface. By adjusting appropriate operating conditions scanning force microscope setup, tip−sample contact avoided during imaging at atmospheric pressure. This allows study even softest samples. In present work, we demonstrate molecularly thin water films imaged with nanometer resolution on different substrates such as mica,...
This manuscript describes a novel method for the biofunctionalization of glass surfaces with polyhistidine-tagged proteins. The main innovation this methodology consists covalent binding between nitrilotriacetic acid (NTA) moiety and proteins, ensuring not only orientation, but also stability recombinant proteins on NTA-covered surfaces. In work, as C-terminal polyhistidine tagged cadherin extracellular fragments have been used, guarantees proper orientation these by mimicking their...
In the present work, we describe a technique to measure tip−sample interaction in scanning force microscope setup with high precision. Essentially, exerted on cantilever is acquired simultaneously spectrum of cantilever. This applied study behavior as tip approaches sample surface ambient conditions. The measured can only be understood assuming formation liquid neck and presence thin film well sample.
Tip-sample interaction in intermittent contact scanning force microscopy, also called tapping mode, is experimentally studied to determine under which conditions tip-sample established. Force vs distance curves are made while the cantilever oscillating at its resonance frequency. Cantilevers with different constants driven oscillation amplitudes have been used. In addition, samples hardness, such as silicon oxide, glass, and highly orientated pyrolytic graphite were taken sample surface....
We present a study of the effect gold nanoparticles (Au NPs) on TiO2 charge generation and trapping during illumination with photons energy larger than substrate band gap. used novel characterization technique, photoassisted Kelvin probe force microscopy, to process at single Au NP level. found that photoinduced electron transfer from increases logarithmically light intensity due combined contribution electron-hole pair in space region TiO2-air interface metal-semiconductor junction. Our...
The interaction of water with self-assembled alkylsilane monolayers on mica substrates has been studied using an atomic force microscope operated in contact, noncontact, and electrical polarization modes. Complete monolayer films were found to be effective blocking adsorption. On partially covered surfaces was produce large changes the conductivity surface potential exposed regions. It also that could penetrate near defects at island edges.
Soft and spherical nanoparticles, named as cutinsomes, have been prepared from concentrated 9(10),16-dihydroxypalmitic acid (diHPA) in aqueous solution. After isolation, cutinsomes chemically structurally characterized by ATR-FTIR, TEM dynamic atomic force microscopy (dynamic AFM). The nanoparticle can be described a lipidic, liquid-like mostly esterified core surrounded polar shell of carboxylate/carboxylic molecules. Molecular (MD) simulations used to support this model. structural...
Abstract In this work, the preparation of regular nanosized columnar structures titanium dioxide by means glancing angle deposition with magnetron sputtering (MS‐GLAD) followed thermal annealing is reported. MS‐GLAD gives rise to metallic width and length that after treatment are fully oxidized form TiO 2 nanocolumns maintain morphological features original ones. Further functionalization gold multiple ion cluster source results in well‐dispersed Au nanoparticles across nanocolumns’ surface...
In this work we present a powerful, affordable, and portable biosensor to develop Point of care (POC) SARS-CoV-2 virus detection. It is constructed from fast, low cost, electronically automatized potentiostat that controls the potential applied disposable screen-printed electrochemical platform current response. The was designed get best signal-to-noise ratio, very simple user interface offering possibility be used by any device (computer, mobile phone or tablet), have small size, cheap...
In this work we describe a highly sensitive method based on biocatalyzed electrochemiluminescence approach. The system combines, for the first time, use of few-layer bismuthene (FLB) as platform oriented immobilization tetrahedral DNA nanostructures (TDNs) specifically designed and synthetized to detect specific SARS-CoV-2 gene sequence. one its vertices, these TDNs contain capture probe open reading frame 1 ab (ORF1ab) virus, available biorecognition target DNA/RNA. At other three there are...