A5113959979- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Hepatitis B Virus Studies
- COVID-19 epidemiological studies
- COVID-19 Clinical Research Studies
- Respiratory viral infections research
- Malaria Research and Control
- Influenza Virus Research Studies
- Virology and Viral Diseases
- vaccines and immunoinformatics approaches
- SARS-CoV-2 detection and testing
- Disaster Response and Management
- Immune responses and vaccinations
- Blood groups and transfusion
- Vaccine Coverage and Hesitancy
- Immunodeficiency and Autoimmune Disorders
- Vector-borne infectious diseases
- Glycosylation and Glycoproteins Research
- Animal Virus Infections Studies
- Insect symbiosis and bacterial influences
- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
University of Hawaiʻi at Mānoa
2017-2025
PanThera Biopharma, LLC (United States)
2011-2013
Hawaii Biotech (United States)
2006-2013
Panthera Corporation
2011-2013
Panthera Biopartners
2011-2013
The immunogenicity and protective efficacy of a recombinant subunit West Nile virus (WNV) vaccine was evaluated in rhesus macaques (Macaca mulatta). consisted envelope (E) protein truncated at the C-terminal end, resulting polypeptide containing 80% N-terminal amino acids native WNV (WN-80E), mixed with an adjuvant (GPI-0100). WN-80E produced Drosophila melanogaster expression system high yield purified by immunoaffinity chromatography using monoclonal antibody specific for flavivirus E...
Ebola virus (EBOV), a member of the Filoviridae family, causes most severe form viral hemorrhagic fever. Although no FDA licensed vaccine or treatment against disease (EVD) is currently available, glycoprotein (GP) major antigen used in all candidate vaccines. Recent reports protection as quickly within 6 days administration rVSV-based expressing EBOV GP before robust humoral responses were generated suggests that innate immune elicited early after vaccination may contribute to protection....
The recent outbreaks of Zika virus (ZIKV) infection in French Polynesia, the Caribbean, and Americas have highlighted severe neuropathological sequelae that such an may cause. development a safe, effective ZIKV vaccine is critical for several reasons: (i) difficulty diagnosing active due to common nonspecific symptoms, (ii) lack specific antiviral therapy, (iii) potentially devastating pathological effects utero infection. Moreover, with excellent safety profile, as nonreplicating,...
Ebola (EBOV), Marburg (MARV) and Sudan (SUDV) viruses are the three filoviruses which have caused most fatalities in humans. Transmission from animals into human population typically causes outbreaks of limited scale endemic regions. In contrast, 2013-16 outbreak several West African countries claimed more than 11,000 lives revealing true epidemic potential filoviruses. This is further emphasized by difficulty seen with controlling 2018-2020 EBOV Democratic Republic Congo (DRC), despite...
Dengue fever, dengue haemorrhagic and shock syndrome are caused by infections with any of the four serotypes virus (DENV), an increasing global health risk. The related West Nile (WNV) causes significant morbidity mortality as well, continues to be a threat in endemic areas. Currently no FDA-approved vaccines or therapeutics available prevent treat these infections. Like other members Flaviviridae, DENV WNV encode protease (NS3) which is essential for viral replication therefore promising...
Zika Virus (ZIKV), a virus with no severe clinical symptoms or sequelae previously associated human infection, became public health threat following an epidemic in French Polynesia 2013-2014 that resulted neurological complications infection. Although treatment currently exists, several vaccines using different platforms are development. These include nucleic acid based on the prM-E protein from and purified formalin-inactivated ZIKV (ZPIV) which Phase 1/II trials. Using recombinant subunit...
The emergence of COVID-19 necessitated the rapid development vaccines. While highly effective at reducing severe disease and death, breakthrough infections remain a problem as virus continues to mutate. To help address this issue, we show utility multiplex immunoassay in measuring multiple aspects antibody response generated by SARS-CoV-2 We use platform measure spike-specific IgG concentration, avidity, receptor-binding inhibition. In addition, correlate results from an ACE-2 inhibition...
Insect-borne viruses may account for a significant proportion of non-malaria and non-bacterial febrile illnesses in Liberia. Although the presence many arthropod vectors has been documented, collective burden arbovirus infections baseline pre-existing immunity remains enigmatic. Our goal was to determine seroprevalence exposure across country using resource-sparing, multiplex immunoassay IgG responses immunodominant antigens. 532 human serum samples, from healthy adults, collected 10...
The current COVID-19 pandemic has claimed hundreds of thousands lives and its causative agent, SARS-CoV-2, infected millions, globally. highly contagious nature this respiratory virus spurred massive global efforts to develop vaccines at record speeds. In addition enhanced immunogen delivery, adjuvants may greatly impact protective efficacy a SARS-CoV-2 vaccine. To investigate adjuvant suitability, we formulated protein subunit consisting the recombinant S1 domain Spike alone or in...
The SARS-CoV-2 pandemic ignited global efforts to rapidly develop testing, therapeutics, and vaccines. However, the rewards of these were slow reach many low- middle-income countries (LMIC) across African continent globally. Therefore, two bead-based multiplexed serological assays developed determine exposure four counties in Liberia. This study was conducted during summer 2021 on 189 samples collected throughout Grand Bassa, Bong, Margibi, Montserrado counties. Our immunoassay (MIA)...
Filoviruses such as Ebola virus (EBOV) cause outbreaks of viral hemorrhagic fevers for which no FDA-approved vaccines or drugs are available. The 2014–2016 EBOV outbreak in West Africa infected approximately 30,000 people, killing more than 11,000 and affecting thousands areas still suffering from the effects civil wars. Sierra Leone Liberia reported cases every county demonstrating efficient spread this highly contagious well-connected societies Africa. In communities, canines often contact...
The speed at which several COVID-19 vaccines went from conception to receiving FDA and EMA approval for emergency use is an achievement unrivaled in the history of vaccine development. Mass vaccination efforts using highly effective are currently underway generate sufficient herd immunity reduce transmission SARS-CoV-2 virus. Despite most advanced technology, global recipient coverage, especially resource-poor areas remains a challenge as genetic drift naïve population pockets threatens...
The emergence of SARS-CoV-2 has resulted in a significant impact on public health, particularly for individuals with underlying health conditions such as obesity and diabetes. While vaccination efforts have played crucial role reducing hospitalizations, it remains unclear whether the effectiveness these vaccines varies among different population groups. In this study, we investigated immune responses generated by various vaccine platforms mouse models diabetes, focusing both cell-mediated...
Although West Nile Virus (WNV) has not been reported in Hawai'i, eventual introduction appears unavoidable with potential adverse effects on avian species. Nēnē (Branta sandvicensis) are endemic endangered Hawaiian geese that susceptible to WNV. We demonstrate a vaccine developed against WNV for humans (WN-80E) is also highly immunogenic and does produce biologic effects. Six captive, nonbreeding were immunized two 10-μg doses (4 wk apart) of the WN-80E recombinant protein adjuvanted...
FDA-approved and emergency use-authorized vaccines using new mRNA viral-vector technology are highly effective in preventing moderate to severe disease; however, information on their long-term efficacy protective breadth against acute respiratory syndrome coronavirus 2 variants of concern (VOCs) is currently scarce. Here, we describe the durability broad-spectrum VOC immunity a prefusion-stabilized spike (S) protein adjuvanted with liquid or lyophilized CoVaccine HT cynomolgus macaques. This...
ABSTRACT The speed at which several COVID-19 vaccines went from conception to receiving FDA and EMA approval for emergency use is an achievement unrivaled in the history of vaccine development. Mass vaccination efforts using highly effective are currently underway generate sufficient herd immunity reduce transmission SARS-CoV-2 virus. Despite most advanced technology, global recipient coverage, especially resource-poor areas remains a challenge as genetic drift naïve population pockets...
Zika virus (ZIKV) is an emerging flavivirus associated with several neurological diseases such as Guillain-Barré syndrome in adults and microcephaly newborn children. Its distribution mode of transmission (via Aedes aegypti albopictus mosquitoes) collectively cause ZIKV to be a serious concern for global health. High genetic homology flaviviruses shared ecology hurdle accurate detection. Distinguishing infections caused by different viruses based on serological recognition can misleading...