Silicon dioxide surfaces, functionalized by two aminosilane compounds (3-amino-propyl-triethoxysilane, APTES; 3-amino-propyl-dimethyl-ethoxysilane, APDMES) both dissolved in different solvents (dry ethanol and toluene), have been investigated standard techniques such as spectroscopic ellipsometry (SE), water contact angle (WCA), atomic force microscopy (AFM). Silane thicknesses between 5 80 Å found, depending on deposition conditions; surface wettabilities change, accordingly. These...

Human α-thrombin (TB) is a serine protease with crucial role in coagulation and hemostasis.

Fabrication and characterization of an optically monitored hybrid patch for local administration drugs, based on polymeric micro-needles a porous silicon free-standing membrane, are reported.The microneedles realized by innovative photolithographic approach that allows fine tuning geometrical parameters, using polyethylene glycol commercial photo-catalyzer.The multilayer not only increases the storage relevant amount drug, but also offers continuous, naked-eye monitoring drug delivery...

Phytochelatins are small peptides that can be found in several organisms, which use these oligopeptides to handle heavy metal elements. Here, we report a method for monitoring interactions between lead(ii) ions aqueous solutions and phytochelatin 6 oligopeptide bioconjugated onto pegylated gold nanorods (PEG-AuNrs). This study is the first step towards high sensitive label free optical biosensor quantify pollution water.

Abstract HydrophobinVmh2 is a small amphiphilic protein, which self-assembles on different surfaces and naturally interacts with glucose. Here, we report the synthesis of nanobiocomplex made polyethylene glycol, Vmh2 gold nanoparticles by one-step process its ability to recognise glucose in an aqueous solution at 0.3–0.6–1.2 mg ml −1 concentrations. Even though proteins are intrinsically bonded core, effective interaction monitoring was demonstrated using dynamic light scattering,...

Water sources pollution by arsenic ions is a serious environmental problem all around the world. Arsenate reductase enzyme (TtArsC) from Thermus thermophilus extremophile bacterium, naturally binds ions, As(V) and As (III), in aqueous solutions. In this research, TtArsC adsorption onto hybrid polyethylene glycol-stabilized gold nanoparticles (AuNPs) was studied at different pH values as an innovative nanobiosystem for metal concentration monitoring. Characterizations were performed UV/Vis...

The thermophilic bacterium Thermus thermophilus HB27 encodes chromosomal arsenate reductase (TtArsC), the enzyme responsible for resistance to harmful effects of arsenic. We report on adsorption TtArsC onto gold nanoparticles naked-eye monitoring biomolecular interaction between and arsenic species. Synthesis hybrid biological-metallic has been characterized by transmission electron microscopy (TEM), ultraviolet-visible (UV-vis), dynamic light scattering (DLS) phase modulated infrared...

Graphene oxide (GO) is a photoluminescent material whose application in integrated optoelectronics has been strongly limited due to poor emission intensity and handling procedures not compatible with standard microelectronic ones. In this work, hybrid GO-porous silicon (GO-PSi) structure realized order investigate the properties of GO infiltrated into an aperiodic porous multilayered matrix. A photoluminescence enhancement by factor 32, compared same amount deposited on flat surface,...

Amphiphilic proteins, which self-assemble at solid-liquid interface in nanometric biolayer, such as hydrophobins, can be used multifunctional film to passivate porous silicon dioxide and also sense glucose. Several optical transducers (rugate filter, Thue-Morse sequence, microcavity) have been protein-modified tested monitoring hydrophobins-glucose binding. A simple, easy-to-integrate technique, water contact angle, is able reveal sugar presence 1.2 mg/ml, whereas spectroscopic reflectometry...

Fullerenes, allotropic forms of carbon, have very interesting pharmacological effects and engineering applications. However, a low solubility both in organic solvents water hinders their use. Fullerene C60, the most studied among fullerenes, can be dissolved only form nanoparticles variable dimensions limited stability. Here effect on production C60 by native denatured hen egg white lysozyme, highly basic protein, has been systematically studied. In order to obtain denatured, yet soluble,...

Efficient biorecognition of thrombin (TB), a serine protease with crucial role in physiological and pathological blood coagulation, is hot topic medical diagnostics. In this work, we investigate the ability synthetic aptamer (TBA), immobilized on gold substrate, to bind by two different label-free techniques: quartz crystal microbalance (QCM) spectroscopic ellipsometry (SE). By QCM characterization range from 20 110 nM, demonstrate high specificity TBA-TB interaction determine affinity...

In a broader scenario, the forced degradation studies provided by ICH guidelines for Q1A, Q1B, and Q2B allow to know CQA of molecule used as drug product, determine appropriate analytical methods, excipients, storage conditions ensuring quality drug, its efficacy, patient safety. this study, we focused our attention on understanding how oxidative stress is performed H2 O2 -impacted small synthetic peptides that do not contain residues susceptible oxidation such methionine. Among amino acids...

The development of simple, rapid, and low cost methods for early detection, identification, measurement multiple biomarkers remains a challenge to improve diagnosis, treatment monitoring, prognosis cancer. Biosensing technology, combining the properties biological systems with functional advanced materials, guarantees reproducible, highly sensitive cell detection. In this study, we developed silicon-based biochips prostate cancer PC3 cells detection by using cytokeratin 8/18 Urotensin...

Microneedles are newly developed biomedical devices, whose advantages mainly in the non-invasiveness, discretion and versatility of use both as diagnostics therapeutics tool. In fact, they can be used for drugs delivery interstitial fluids analysis fluid. this work we present preliminary results two devices based on micro needles PolyEthylene (Glycol). The first includes a membrane optical reflected wavelength is related to concentration drug. Here, our result diffusion between microneedles....

Applications of microneedles in biomedicine wide range from diagnostics to therapeutics. This crucial and versatile tool is the interface between human body often a complicated device. We present two sensing device based on hybrid array for diagnostic therapeutic applications. The was fabricated by using commercial photocatalyzer solidify liquid PolyEthylene Glycol hydrogel. After polymerization, MNs have porous structure, which can include variety biological molecules, as bioprobes or...

A proper bioconjugation of molecular probes onto a transducer surface is key point in developing new kind biosensors. Porous Silicon (PSi) and quartz resonators (QR) are commonly used as transducers biochemical sensors using spectroscopic reflectometry crystal microbalance (QCM). In this work, we studied oligopeptides, namely Phytochelatins (PCs), which able to bind heavy metal aqueous solutions, both on PSi QR surfaces: strong competition between protein-binding protein-solubilization...

Nanostructured photoluminescent materials are optimal transducers for optical biosensors due to their capacity convert molecular interactions in light signals without contamination or deterioration of the samples. In recent years, nanostructured with low cost and readily available properties have been developed such applications as therapeutics, diagnostic environmental. Zinc oxide nanowires (ZnO NWs) is material unique these they were widely studied many fields electronics, optics,...

Nanostructured materials, due to their peculiar physical and chemical properties, constitute the new class of functional supports for specific innovative applications. There is a strong competition between synthetic, man-made nanostructured materials those coming directly from nature: first ones can be precisely tailored by properly design fabrication; second are available without any cost production. In this paper, we report our latest achievements on different porous silicon, zinc oxide...