A5090944106- Malaria Research and Control
- Computational Drug Discovery Methods
- Mosquito-borne diseases and control
- Drug Transport and Resistance Mechanisms
- Parasites and Host Interactions
- Reproductive System and Pregnancy
- Ubiquitin and proteasome pathways
- Pregnancy and preeclampsia studies
- Nanoparticle-Based Drug Delivery
- Pharmacological Effects and Toxicity Studies
- Peptidase Inhibition and Analysis
- RNA Interference and Gene Delivery
- Epilepsy research and treatment
- Microbial Metabolites in Food Biotechnology
- Immune responses and vaccinations
- Antibiotic Resistance in Bacteria
- Research on Leishmaniasis Studies
- HIV/AIDS drug development and treatment
- DNA Repair Mechanisms
- Neonatal Respiratory Health Research
- Antibiotics Pharmacokinetics and Efficacy
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- HIV Research and Treatment
- Hemoglobinopathies and Related Disorders
Wellcome Sanger Institute
2018-2025
Cleveland Clinic
2023-2024
National Institute of Allergy and Infectious Diseases
2018-2023
National Institutes of Health
2018-2023
University of Cambridge
2022
Denison University
2014
National Cancer Institute
2013
Center for Cancer Research
2013
Deubiquitinating enzymes function to cleave ubiquitin (Ub) moieties from modified proteins, serving maintain the pool of free Ub in cell while simultaneously impacting fate and a target protein. Like all eukaryotes, Plasmodium parasites rely on dynamic addition removal for their own growth survival. While humans possess around 100 deubiquitinases, contains ∼20 putative hydrolases, many which bear little no resemblance those other organisms. In this study, we characterize falciparum UBP-1,...
ABCB1, also known as P-glycoprotein (P-gp) or multidrug resistance protein 1 (MDR1), is a membrane-associated transporter of the ATP-binding cassette (ABC) family. It one most widely studied transporters that enable cancer cells to develop drug resistance. Reliable high-throughput assays can identify compounds interact with ABCB1 are crucial for developing new therapeutic drugs. A assay measuring ABCB1-mediated calcein AM efflux was developed using fluorescent and phase-contrast live cell...
Artemisinin derivatives and their partner drugs in artemisinin combination therapies (ACTs) have played a pivotal role global malaria mortality reduction during the last two decades. The loss of efficacy due to evolving drug-resistant parasites could become serious health threat. Dihydroartemisinin-piperaquine is well tolerated generally highly effective ACT. implementation drug ACTs critical control emerging resistance. Even though parasite clearance, it labile human body. A necessary for...
Abstract Background Long regarded as an epicenter of drug-resistant malaria, Southeast Asia continues to provide new challenges the control Plasmodium falciparum malaria. Recently, resistance artemisinin combination therapy partner drug piperaquine has been observed in multiple locations across Asia. Genetic studies have identified single nucleotide polymorphisms well copy number variations plasmepsin 2 and 3 genes, which encode haemoglobin-degrading proteases that associate with clinical...
Drug-resistant
Histone post-translational modifications have been shown to contribute DNA damage repair. Prior studies suggested that specific H3K79 methylation states play distinct roles in the response UV-induced damage. To evaluate these observations, we examined effect of altered patterns on G1/S checkpoint and sister chromatid exchange (SCE). We found di- trimethylated both activation varying degrees, depending synchronization method, although is not required for high levels UV In contrast, SCE...
Introduction Maternal intervillous monocytes (MIMs) and fetal Hofbauer cells (HBCs) are myeloid-derived immune at the maternal-fetal interface. reproductive history is associated with differential risk of pregnancy complications. The molecular phenotypes roles these distinct monocyte/macrophage populations influence gravidity on has not been systematically investigated. Methods Here, we used RNA sequencing to study transcriptional profiles MIMs HBCs in normal term pregnancies. Results Our...
The bacterial heterodimeric ATP-binding cassette (ABC) multidrug exporter PatAB has a critical role in conferring antibiotic resistance multidrug-resistant infections by Streptococcus pneumoniae. As with other ABC exporters, contains two transmembrane domains that form drug translocation pathway for efflux and nucleotide-binding bind ATP, one of which is hydrolysed during transport. structural functional elements exporters determine interactions drugs couple binding to nucleotide hydrolysis...
The first-line treatments for uncomplicated Plasmodium falciparum malaria are artemisinin-based combination therapies (ACTs), consisting of an artemisinin derivative combined with a longer acting partner drug. However, the spread P. decreased susceptibility to and drugs presents significant challenge control efforts. To stem drug resistant parasites, novel chemotherapeutic strategies being evaluated, including implementation triple (TACTs). Currently, there is limited knowledge on...
Abstract Maternal intervillous monocytes (MIMs) and fetal Hofbauer cells (HBCs) are myeloid-derived immune at the maternal-fetal interface. Little is known regarding molecular phenotypes roles of these distinct monocyte/macrophage populations. Here, we used RNA sequencing to investigate transcriptional profiles MIMs HBCs in six normal term pregnancies. Our analyses revealed transcriptomes HBCs. Genes involved differentiation cell organization pathways were more highly expressed vs. In...
Abstract Long regarded as an epicenter of drug-resistant malaria, Southeast Asia continues to provide new challenges the control Plasmodium falciparum malaria. Recently, resistance artemisinin combination therapy partner drug piperaquine has been observed in multiple locations across Asia. Genetic studies have identified a single nucleotide polymorphism well copy number variation molecular marker that associate with clinical and vitro resistance. The is duplication region containing members...
Abstract The first-line treatments for uncomplicated Plasmodium falciparum malaria are artemisinin-based combination therapies (ACTs), consisting of an artemisinin derivative combined with a longer acting partner drug. However, the spread P. decreased susceptibility to and drugs presents significant challenge control efforts. To stem drug resistant parasites, novel chemotherapeutic strategies being evaluated, including implementation triple (TACTs). Currently, there is limited knowledge on...
Abstract ABCB1, also known as P-glycoprotein (P-gp) or multidrug resistance protein 1 (MDR1), is a membrane-associated transporter of the ATP-binding cassette (ABC) family. It one most widely studied transporters that enable cancer cells to develop drug resistance. Reliable high-throughput assays can identify compounds interact with ABCB1 are crucial for developing new therapeutic drugs. A assay measuring ABCB1-mediated calcein AM efflux was developed using IncuCyteFLR technology. Time- and...
ABSTRACT Deubiquitinating enzymes function to cleave ubiquitin moieties from modified proteins, serving maintain the pool of free in cell while simultaneously impacting fate and a target protein. Like all eukaryotes, Plasmodium parasites rely on dynamic addition removal for their own growth survival. While humans possess around 100 DUBs, contains ∼20 putative hydrolases, many which bear little no resemblance those other organisms. In this study, we characterize Pf UBP-1, large hydrolase...