A5057305848- Electrochemical Analysis and Applications
- Force Microscopy Techniques and Applications
- Analytical Chemistry and Sensors
- Lipid Membrane Structure and Behavior
- Electrochemical sensors and biosensors
- Nanopore and Nanochannel Transport Studies
- Alzheimer's disease research and treatments
- Computational Drug Discovery Methods
- Microtubule and mitosis dynamics
- Conducting polymers and applications
- Microfluidic and Bio-sensing Technologies
- Ion-surface interactions and analysis
- Neuroscience and Neuropharmacology Research
- Cholinesterase and Neurodegenerative Diseases
- Nuclear Structure and Function
- Trace Elements in Health
- S100 Proteins and Annexins
- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Radiopharmaceutical Chemistry and Applications
- bioluminescence and chemiluminescence research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Biochemical and Structural Characterization
- Prostate Cancer Treatment and Research
- Industrial and Mining Safety
Lomonosov Moscow State University
2019-2024
National University of Science and Technology
2020-2024
National University of Science and Technology
2019-2024
Moscow State University
2021
Skolkovo Foundation
2019
Mechanical properties of living cells determined by cytoskeletal elements play a crucial role in wide range biological functions.
β-Amyloid aggregation on living cell surfaces is described as responsible for the neurotoxicity associated with different neurodegenerative diseases. It suggested that of β-amyloid (Aβ) peptide neuronal surface leads to various deviations its vital function due myriad pathways defined by internalization calcium ions, apoptosis promotion, reduction membrane potential, synaptic activity loss, etc. These are structural reorganizations and pathologies cytoskeleton mainly involving actin...
Mechanical properties of living cells play a crucial role in wide range biological functions and pathologies, including atherosclerosis. We used low-stress Scanning Ion-Conductance Microscopy (SICM) correlated with confocal imaging demonstrated the topographical changes mechanical alterations EA.hy926 THP-1 exposed to LDL extracted from CVD patients’ blood samples. show that stiffened presence LDL, which also triggered caveolae formation. Endothelial accumulated less cholesterol form lipid...
Prostate cancer is the second most common type of among men. Its main method treatment chemotherapy, which has a wide range side effects. One solutions to this challenge targeted delivery prostate cells. Here we synthesized novel small-molecule PSMA-targeted conjugate based on monomethyl auristatin E. structure and conformational properties were investigated by NMR spectroscopy. Cytotoxicity, intracellular reactive oxygen species induction, stability under liver microsomes P450-cytochrome...
The reactive oxygen species (ROS) production by a single neutrophil after stimulation with S. aureus and E. coli was estimated an electrochemical amperometric method high time resolution. This showed significant variability in the response of to bacterial stimulation, from "silent cell" pronounced manifested series chronoamperometric spikes. amount ROS produced under influence 5.5-fold greater than that coli. granulocyte population analyzed using luminol-dependent biochemiluminescence (BCL)....
Peptide-based hydrogels were shown to serve as good matrices for 3D cell culture and be applied in the field of regenerative medicine. The study cell-matrix interaction is important understanding attachment, proliferation, migration, well improvement matrix. Here, we used scanning ion conductance microscopy (SICM) growth cells on self-assembled peptide-based hydrogels. hydrogel surface topography, which changes during its formation an aqueous solution, studied at nanoscale resolution...
Clathrin-mediated endocytosis (CME) is an essential cell physiological process of broad biomedical relevance. Since the recent introduction Pitstop-2 as a potent CME inhibitor, we and others have reported on substantial clathrin-independent inhibitory effects. Herein, developed experimentally validated novel fluorescent derivative Pitstop-2, termed RVD-127, to clarify diverse Using were able trace additional protein targets Pitstop-2. Besides inhibiting CME, RVD-127 proved directly...
Mechanical properties of neuronal cells have a key role for growth, generation traction forces, adhesion, migration, etc. are regulated by chemical signaling, neurotransmitters, and ion exchange. Disturbance signaling is accompanied several diseases such as ischemia, trauma, neurodegenerative diseases. It known that the disturbance like caused glutamate excitotoxicity, leads to structural reorganization cytoskeleton deviation native mechanical properties. Thus, investigate living in presence...
The study of individual cell processes that occur both on their surface and inside is highly interesting for the development new medical drugs, cytology technologies. This work presents an original technique fabricating silver-coated pipette its use analysis by combination with surface-enhanced Raman spectroscopy (SERS) scanning ion-conducting microscopy (SICM). Unlike majority other designs, opening in our case remains uncovered, which important SICM. SERS-active Ag nanoparticles are formed...
Abstract Alzheimer’s disease (AD) is the most common form of dementia, a progressive neurological disorder characterized by short and long-term memory loss, including cognitive functional impairment, which refractory to current therapy. It suggested that aggregation β-amyloid (Aβ) peptide on neuronal cell surface leads various deviations its vital function due myriad pathways defined internalization calcium ions, apoptosis promotion, reduction membrane potential, synaptic activity loss etc....
Super-resolution microscopy is widely used in the development of novel antimicrobial testing vitro. In presented work, a scanning protocol was developed by method ion-conducting (SICM), which makes it possible to study microorganisms without rigid fixation and saline, obtaining an index map nanosized structures. The effect azole echinocandins drugs on morphology mechanical properties Candida parapsilosis yeast studied. findings are consistent with previously proposed drug mechanisms reports...
An abstract is not available for this content so a preview has been provided. As you have access to content, full PDF via the 'Save PDF' action button.
A high-precision system was developed for the quantification of biological analytes in single cells (reactive oxygen species (ROS) and reactive nitrogen (RNS)) based on electrochemical amperometric method. The efficacy this evaluated using an experimental bacteremia model. Endothelial exhibited increased ROS/RNS production when stimulated by Staphylococcus aureus. However, they remained inactive exposed to either unprimed or primed neutrophils that had been pre-incubated with bacteria. It is...
Five bifunctional copper chelating agents,
The morphological and mechanical properties of hydrogels with different stiffness have been assessed by SICM AFM.
Post-translational modifications of beta-amyloid (Aβ) play an important role in the pathogenesis Alzheimer’s disease (AD). Aβ such as Ser8 phosphorylation (pS8-Aβ 42 ) and Asp7 isomerization (iso-Aβ can significantly alter properties have been detected vivo . One reasons for different pathogenicity isoforms may be activation signaling cascades leading to changes mechanical cells. In this paper, we used correlative scanning ion-conductance microscopy (SICM) Pt-nanoelectrodes compare effects...
Abstract This study aims to investigate the potential role of antioxidants in oxidative stress and its consequent impact on mechanical properties neuronal cells, particularly induced by amyloid‐beta (1–42) (Aβ 42 ) aggregates. A key aspect our research involved using scanning ion‐conductance microscopy (SICM) assess (Young's modulus) cells under stress. Reactive oxygen species (ROS) level was measured single‐cell electrochemical method low‐invasive Pt nanoelectrode. We investigated effects...
Biomimetic hydrogels have garnered increased interest due to their considerable potential for use in various fields, such as tissue engineering, 3D cell cultivation, and drug delivery. The primary challenge applying engineering is accurately evaluating mechanical characteristics. In this context, we propose a method using scanning ion conductance microscopy (SICM) determine the rigidity of living human breast cancer cells MCF-7 grown on soft, self-assembled Fmoc-FF peptide hydrogel....
An abstract is not available for this content so a preview has been provided. As you have access to content, full PDF via the 'Save PDF' action button.
Alz-5 acts as a bifunctional chelator that can interact with various Aβ aggregates and reduce their neurotoxicity. Single-cell ROS measurements provided by Pt-nanoelectrode technique revealed significant antioxidant properties of in 42 - affected SH-SY5Y cell. AFM data obtained on fibrils clearly indicate an anti-aggregating property Alz-5. Young’s modulus mapping living cells ability to decrease cell rigidity cells.