Aerobic glycolysis has pleiotropic roles in the pathogenesis of hepatocellular carcinoma (HCC).Emerging studies revealed key promoters aerobic glycolysis, however, little is known about its negative regulators HCC.In this study, an integrative analysis identifies a repertoire differentially expressed genes (DNASE1L3, SLC22A1, ACE2, CES3, CCL14, GYS2, ADH4, and CFHR3) that are inversely associated with glycolytic phenotype HCC.ACE2, member rennin-angiotensin system, to be downregulated HCC...

Aim: To identify intestinal microbiota compositions in elderly functional constipation (FC) patients. Materials & methods: Fecal samples from 61 FC patients and 48 healthy age-matched volunteers were analyzed through 16S rRNA gene sequencing. Results: The of significantly different controls. Additionally, the species diversity controls was greater than that Indeed, abundance Firmicutes Proteobacteria decreased, whereas Bacteroides, Prevotella, Lactococcus, Ruminococcus Butyricimonas...

Extreme climate events and nitrogen (N) deposition are increasingly affecting the structure function of terrestrial ecosystems. However, response plant biomass to variations these global change drivers is still unclear in semi-arid regions, especially degraded sandy grasslands. In this study, a manipulative field experiment run over two years (from 2017 2018) was conducted examine effect rainfall alteration addition on allocation annuals perennial plants Horqin grassland, Northern China. Our...

Phlomoides rotata, a traditional medicinal plant, is commonly found on the Tibetan Plateau at altitudes of 3100-5200 m. Its primary active compounds, flavonoids and phenylethanol glycosides (PhGs), exhibit various pharmacological effects, including hemostatic, anti-inflammatory, antitumor, immunomodulatory, antioxidant activities. This study analyzed flavonoid PhG metabolites in roots P. rotata collected from Henan County (HN), Guoluo (GL), Yushu (YS), Chengduo (CD) Qinghai Province. A total...

Phlomoides rotata, a traditional medicinal plant, always grows on the Tibetan Plateau at high altitude of 3100–5200 m. The major active ingredients in P. rotata were used medicines due to their diverse pharmacological effects, including hemostatic, anti-inflammatory, antitumor, immuno-modulatory, and antioxidant activities. This study screened 15 top endophytic genus through analysis OTUs 30 metabolites with relatively content roots from four different habitats (HN, GL, YS, CD regions)...

Iodine 125 (125I) seed irradiation is an effective treatment for unresectable pancreatic cancers. However, the radiobiological mechanisms underlying brachytherapy remain unclear. Therefore, we investigated influence of continuous and low-energy 125I on apoptosis, expression DNA methyltransferases (DNMTs) cell growth in cancers.For vitro irradiation, SW-1990 cells were divided into three groups: control (0 Gy), 2 Gy, 4 Gy. To create animal model cancer, SW 1990 surgically implanted mouse...

Objective:The fecal microbiota was studied in patients with inflammatory bowel disease (IBD), and the characteristics of gut were compared among different subtypes stages IBD, aiming to identify associated IBD.Methods: Fecal samples collected from 41 IBD (18 ulcerative colitis [UC] 23 Crohn's [CD]) Department Gastroenterology East China Hospital, Fudan University between January 2021 2022.In addition, 20 healthy volunteers.The subjected 16S rRNA gene sequencing, followed by bioinformatics...

This study aimed to investigate the characteristics of intestinal flora in patients with chronic functional constipation before and after lactulose intervention. Twenty-nine treatment group received oral (15 mL/d) for a month. Twenty healthy subjects served as controls. Stool specimens were collected treatment. Fecal bacteria examined by 16SrRNA gene sequencing bioinformatics analysis. After treatment, most group, including Bifidobacteria, Bacillus cereus, Prevotella, Bacillus, Anaerostipes,...

Ecological degradation is a global problem, and ecological restoration technologies have played will continue to play an important role in its mitigation. However, the lack of systematic research evaluations has thus far affected their effective application vulnerable regions. This study therefore provides overview main for remediating soil water erosion, desertification, rock desertification China throughout world. It addresses key issues recommends approaches evaluating technologies....

is acknowledged for its substantial nutritional benefits, particularly attributed to the high levels of ascorbic acid (AsA) found in fruits. The "Mengqi No.1" variety

During 2006–2008, 572 isolates of Phytophthora capsici were collected from seven provinces in China, and their sensitivities to three carboxylic acid amides (CAA), dimethomorph, flumorph pyrimorph determined. Of these isolates, 90 without a history exposure CAA fungicides (CAAs) used set up the baseline sensitivity. Baseline EC50 values ranged 0.122 0.203 (mean ± SD, 0.154 0.022) μg ml−1 for 0.301 0.487 0.373 0.043) 0.557 0.944 0.712 0.082) pyrimorph, respectively. The other 482 tested with...

The phosphoinositide 3 kinase (PI3K)/Akt/mammalian target of the rapamycin (mTOR) pathway plays a significant role in colorectal adenocarcinoma. NVP-BEZ235 (dactolisib) is novel dual inhibitor PI3K/mTOR. effects human adenocarcinoma are still unclear. In present study, we aimed to explore proliferation, migration, apoptosis and autophagy HT-29 cells. cells were treated with (0, 0.001, 0.01, 0.1, 1 µM) for 24 48 h, respectively. Cells also (0.1 µM), DDP (100, 300 1,000 combined respectively,...

Abstract Litchi downy blight caused by Peronophythora litchii is one of the most destructive diseases suffered litchi in China. This study has evaluated activities fungicides dimethomorph (DMM), azoxystrobin (AZB), famoxadone (FMD), metalaxyl (MTL), cymoxanil (CYX) and mancozeb (MCB) on mycelial growth, sporulation, zoospores release, germination sporangia as well cystospores P. . Dimethomorph MTL inhibited growth more effectively than other tested. Mycelial was affected less MCB, only...

This study was to explore the influence of flax seeds on gut microbiota elderly patients with functional constipation.Sixty (68.68±8.73 years) constipation were recruited between January 2018 and March 2018. They received oral (50 g/d) for one month. Bowel habits adverse events recorded before after treatment. Fresh stool collected treatment amplification product 16S rRNA V5 region sequenced using next-generation sequencing technique Ion Torrent PGM platform. The analyzed in same...

Four isolates of Peronophythora litchii with resistance to carboxylic acid amide (CAA) fungicides were selected on fungicide-amended agar. These had various levels resistance, as evidenced by their factor (RF), which is the 50% effective concentration (EC 50 ) value a particular isolate divided that wild-type parent. RF values dimethomorph for four 15, 24, 141, and >1,500. Resistance was stable two isolates, while EC decreased other after repeated subculturing fungicide-free medium....

During 2007 and 2008, 392 isolates of Plasmopara viticola were collected from 11 regions in seven provinces China, their sensitivities to metalaxyl dimethomorph determined by the floating leaf disk technique. Among all isolates, 13% classified as sensitive, 26% low-level resistant, 61% resistant metalaxyl. Of 392, 85 vineyards never treated with carboxylic acid amide fungicides; these used determine baseline sensitivity dimethomorph, EC50 values ranged 0.01 0.21 (mean ± SD, 0.11 0.04) μg/ml....

ABSTRACT We carried out an experiment to extract ecological‐restoration‐technology‐related entities (i.e., related the fragile ecological remediation technology of desertification, rocky water and soil erosion control areas, place entities, time performed,) from full‐text documents published in CNKI 1978 2017. Based on extraction we analyzed technologies with greatest potential among China's restoration using category clustering LDA distribution evolution treatment technologies. This...

BACKGROUND The function of long non-coding RNA (lncRNA) BMP/OP-responsive gene (BORG) has only been studied in breast cancer. We analyzed the role BORG colorectal cancer (CRC). MATERIAL AND METHODS CRC tissues and non-cancer from 66 patients was detected by performing quantitative reverse-transcription PCR (RT-qPCR). plasma at 3 times-points: before treatment 6 months after treatment. p53 expression tumor also RT-qPCR. QPCR performed to confirm overexpression cells both cell lines. RESULTS...

Objective Ulcerative colitis (UC), a chronic and refractory nonspecific inflammatory bowel disease, affects millions of patients worldwide increases the risk colorectal cancer. Teprenone is an acylic polyisoprenoid that exerts anti-inflammatory properties in rat models peptic ulcer disease. This vitro vivo study was designed to investigate effects teprenone on UC explore underlying mechanisms.