A5081449924- Retinoids in leukemia and cellular processes
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Crystallization and Solubility Studies
- Biotin and Related Studies
- X-ray Diffraction in Crystallography
- Cellular transport and secretion
- Retinal Development and Disorders
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- Metabolism and Genetic Disorders
- Antioxidant Activity and Oxidative Stress
- Gout, Hyperuricemia, Uric Acid
- Enzyme Structure and Function
- Protein Kinase Regulation and GTPase Signaling
- Insect Utilization and Effects
- Peptidase Inhibition and Analysis
- Antimicrobial Peptides and Activities
- Probiotics and Fermented Foods
- Crystal structures of chemical compounds
- Food Allergy and Anaphylaxis Research
- Porphyrin Metabolism and Disorders
- Biochemical and Molecular Research
University of Parma
2015-2024
Shura Council
2015
Knowledge Unlatched (Germany)
2015
European Synchrotron Radiation Facility
2008
Nara Institute of Science and Technology
2005
University of Pavia
2001
Université Joseph Fourier
2000
Institut de Biologie Structurale
2000
Centre National de la Recherche Scientifique
2000
CEA Grenoble
2000
Maturation of dendritic cells (DCs) is a critical step for the induction an immune response. We have examined role retinoid nuclear receptor pathways in this process. Retinoids induce DC apoptosis, absence inflammatory signals, through retinoic acid (RAR)α/retinoic X (RXR) heterodimers. In contrast, via cross talk with cytokines, retinoids increase DNA binding activity factor κB DCs, trigger membrane major histocompatibility complex class II and costimulatory molecule expression,...
Abstract Since 2018, insects have belonged the category of Novel Foods and presence allergens represents one main hazards connected to their consumption, also due potential cross-reactivity with Arthropoda pan-allergens. In present work, allergenicity assessment black soldier fly lesser mealworm was performed a shotgun bottom-up proteomic approach combined in-silico assessment, followed by IgG- IgE-immunoblotting experiments. The peptides identified, filtered for abundance robustness, mainly...
S-allantoin, a major ureide compound, is produced in plant peroxisomes from oxidized purines. Sequence evidence suggested that the Transthyretin-like (TTL) protein, which interacts with brassinosteroid receptors, may act as bifunctional enzyme synthesis of S-allantoin. Here, we show recombinant TTL Arabidopsis thaliana catalyzes two enzymatic reactions leading to stereoselective formation hydrolysis hydroxyisourate through C-terminal Urah domain, and decarboxylation...
The nutritional and physicochemical properties of sorghum proteins starch make the use this cereal for food production challenging. Sprouting is a cost-effective technology to improve functional profile grains. Two drying treatments were used after sprouting investigate whether phase could protein functionalities. Results showed that treatment at lower temperature/longer time (40 °C 12 h) extended enzymatic activity started during compared one performed higher temperature/shorter (50 6 h)....
Two cellular retinol-binding proteins (CRBP I and II) with distinct tissue distributions retinoid-binding properties have been recognized thus far in mammals. Here, we report the identification of a human protein resembling type (55.6% identity) II (49.6% CRBPs, but unique H residue site distinctively different distribution. Additionally, this binding III) exhibits remarkable sequence identity (62.2%) recently identified ι-crystallin/CRBP diurnal gecko Lygodactylus picturatus [Werten, P. J....
Transthyretin (TTR) is an amyloidogenic protein, the potential of which enhanced by a number specific point mutations. The ability to inhibit TTR fibrillogenesis known for several classes compounds, including natural polyphenols, protect native state specifically interacting with its thyroxine binding sites. Comparative analyses interaction and both stilbenoids flavonoids, some their main metabolites have been carried out. A finding this investigation was highly preferential resveratrol...
Three cellular retinol-binding protein (CRBP) types (CRBP I, II, and III) with distinct tissue distributions retinoid binding properties have been structurally characterized thus far. A human protein, whose mRNA is expressed primarily in kidney, heart, transverse colon, shown here to be a CRBP family member (human IV), according amino acid sequence, phylogenetic analysis, gene structure organization, x-ray structural analysis. Retinol IV leads an absorption spectrum from typical holo-CRBP by...
The hydrolytic cleavage of the hydantoin ring allantoin, catalyzed by allantoinase, is required for utilization nitrogen present in purine-derived compounds. allantoinase gene (DAL1), however, missing many completely sequenced organisms able to use allantoin as a source. Here we show that an alternative (puuE) can be precisely identified analyzing its logic relationship with three other genes pathway. novel annotated structure and sequence data bases polysaccharide deacetylase homology...
Human transthyretin (TTR) is an amyloidogenic protein whose mild amyloidogenicity enhanced by many point mutations affecting considerably the amyloid disease phenotype. To ascertain whether high potential of TTR variants may be explained on basis conformational change hypothesis, aim this work was to determine structural alterations for five crystallized under native and/or destabilizing (moderately acidic pH) conditions. While at pH changes more significant because a higher local...
In plasma the thyroid hormone-binding protein transthyretin (TTR) forms a tight complex with specific retinol carrier retinol-binding (RBP).The Ile-84 + Ser mutation and several other point mutations in TTR are associated familial amyloidotic polyneuropathy, which is characterized by extracellular depositions of amyloid fibrils mainly consisting mutated "RS.The interactions human RBP recombinant normal Ser-84 TTRs were investigated monitoring fluorescence anisotropy RBP-bound retinol.A...
The complete degradation of uric acid to (S)-allantoin, as recently elucidated, involves three enzymatic reactions. Inactivation by pseudogenization the genes pathway occurred during hominoid evolution, resulting in a high concentration urate blood and susceptibility gout. Here, we describe 1.8Å resolution crystal structure homodimeric 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase, which catalyzes last step pathway, for both ligand-free enzyme complex with substrate analogs...
Transthyretin is a tetrameric binding protein involved in the transport of thyroid hormones and cotransport retinol by forming complex plasma with retinol‐binding protein. In present study, we report crystal structure macromolecular complex, which human transthyretin, holo‐retinol‐binding murine anti‐retinol‐binding Fab are assembled according to 1 : 2 stoichiometry. The main interactions, both polar apolar, between transthyretin involve hydroxyl group limited number solvent exposed...
Human transthyretin (TTR) represents a notable example of an amyloidogenic protein, and several compounds that are able to stabilize its native state have been proposed as effective drugs in the therapy TTR amyloidosis. The two thyroxine (T4) binding sites present tetramer display negative cooperativity. Here, structures complex with three natural polyphenols (pterostilbene, quercetin apigenin) determined, which this asymmetry manifests itself presence main site clear ligand occupancy...
Urate oxidase (Uox) catalyses the first reaction of oxidative uricolysis, a three-step enzymatic pathway that allows some animals to eliminate purine nitrogen through water-soluble compound. Inactivation in hominoids leads elevated levels sparingly soluble urate and puts humans at risk hyperuricemia gout. The uricolytic activities lost during evolution can be replaced by enzyme therapy. Here we report on functional structural characterization Uox from zebrafish effects missense mutation...
Plasmids carry genes that give bacteria beneficial traits and allow them to survive in competitive environments. In many cases, they also harbor toxin-antitoxin (TA) systems necessary for plasmid maintenance. TA are generally characterized by a stable "toxin", protein or peptide capable of killing the cell upon loss an unstable "antitoxin", non-coding RNA inhibits toxin activity. Here we report data toward identification RNA-regulated system DNA L. rhamnosus isolated from cheese. The...
Insect consumption could address the increasing protein demand in compliance with environmental sustainability. Hermetia illucens (black soldier fly, BSF) is a promising insect for human diet and it essential to assess related allergenic risk, meant as primary sensitization or cross-reactivity known allergens. In this work, we investigate allergenicity of two tropomyosin variants identified BSF genome produced recombinant proteins. Immunoblot experiments showed that both proteins were...