Protein hydrolysates from lesser mealworm (Alphitobius diaperinus, LM) were obtained by enzymatic hydrolysis with protease Bacillus licheniformis. A preliminary test performed for five hours of generated an insect protein hydrolysate 15% degree (DH), optimum solubility property and oil holding capacity, but emulsifying foaming ability completely impaired. In order to investigate the potential implication DH on techno-functional properties, a set different was sub-sampling at time points...

Abstract Since 2018, insects have belonged the category of Novel Foods and presence allergens represents one main hazards connected to their consumption, also due potential cross-reactivity with Arthropoda pan-allergens. In present work, allergenicity assessment black soldier fly lesser mealworm was performed a shotgun bottom-up proteomic approach combined in-silico assessment, followed by IgG- IgE-immunoblotting experiments. The peptides identified, filtered for abundance robustness, mainly...

Insect proteins have been proposed as a promising alternative for feed and food formulations. In the present work protease-assisted extraction was studied way to separate extract from two different insect species: Alphitobius diaperinus (AD) Hermetia illucens (HI). The proteolytic activity of seven enzymes (papain, pancreatin, dispase I, pepsin, protease Bacillus licheniformis, bromelain trypsin) evaluated determining protein yield, degree hydrolysis (DH) released free amino acids (FAA)....

This research evaluated the application of a one-pot enzymatic extraction by using protease for concomitant and sustainable oils proteins from fruit seeds/kernels different species stone, citrus exotic fruits. The proteolysis improved oil solvent-extractability some compared to use acid and/or organic solvents led directly recover fat (10-33%) mango, lemon pumpkin seeds. Good protein yields were obtained conventional solvent extractions with good hydrolysis degree (almost 10%) in case seed...

Mealworm (MWP), migratory locust (LP), and house cricket (CP) are novel foods recently authorized by the European Commission. This work tested their powders as meat extenders at 5% inclusion in beef burgers. Insect were abundant phenolics, recording highest values LP (1184.9 μg/g). The sensory analysis highlighted a higher visual olfactory acceptability for MWP-burgers, followed CP- LP-burgers, whereas texture of cooked burgers remained unaffected. Following pan-cooking, MWP-burgers control...

Insects are considered a suitable alternative feed for livestock production and their use is nowadays regulated in the European Union by Commission Regulation No. 893/2017. have ability to grow on different spectrum of substrates, which could be naturally contaminated mycotoxins. In present work, mycotoxin uptake and/or excretion two insect species, Alphitobius diaperinus (Lesser Mealworm, LM) Hermetia illucens (Black Soldier Fly, BSF), grown was evaluated. Among all substrates growth...

The interest in agri-food residues and their valorization has grown considerably, many of them are today considered to be valuable, under-exploited sources different compounds notably proteins. Despite the beneficial properties legumes by-products, there also some emerging risks consider, including potential allergenicity. In this work immunoreactivity chickpea, pea, white bean by-products was assessed, whether production enzymatic hydrolysates can an effective strategy reduce allergenic...

The use of chemical pesticides to control the occurrence mycotoxigenic fungi in crops has led environmental and human health issues, driving agriculture sector a more sustainable system. Biocontrol agents such as Bacillus strains their antimicrobial metabolites have been proposed alternatives pesticides. In present work, broth obtained from commercial product containing subtilis QST 713 was tested for its ability inhibit growth well reduce mycotoxin production. Mass spectrometry analysis...

Black soldier fly larvae (BSFL) represent a way of converting organic substrates into valuable biomolecules, and are potentially exploitable as feed food. In the present work, BSFL grown on retted hemp fiber were chemically analyzed to evaluate their nutritional profile. Chemical analysis revealed biomass be an interesting source proteins (40% dry matter) rich in essential amino acids. addition, larval contained 12% fat, mainly composed saturated fatty acids, β-sitosterol campesterol found...

Italian grape ale (IGA) beers have been categorized by the Beer Judge Certification Program as a sub-category of fruit in which grape, or must, is added during brewing process to provide additional characteristics final beer. In present work, IGA produced with must and pomace Malvasia di Candia Aromatica (MaCA) variety, were before fermentation at two different percentages (10% 20%). The (poly)phenolic profile characterized HPLC-MS/MS compared golden control beer (produced same conditions...

Today, alternative strategies based on the use of bioactive compounds have been proposed to reduce mycotoxin contamination and limit chemical fungicides.In present work, several by-products collected from agri-food chain (i.e., red white grape marc, grapevine leaves, seeds stalks, pear, apple, green beans, tomato, spent hops) were subjected extraction protocols steam distillation, Ultrasound-Assisted, Naviglio® extraction) obtain extracts rich in polyphenols terpenes. Each extract was...

A commercial olive leaf extract (OL), effective against Salmonella enterica, Escherichia coli, Listeria monocytogenes, and Staphylococcus aureus, was added to three different coating formulations (methylcellulose, MC; chitosan, CT; alginate, ALG) produce active polylactic acid (PLA) coated films. Evaluation of these PLA films revealed significant inhibition S. aureus growth, particularly with the MC CT exhibiting highest rates (99.7%). The were then tested for food contact compatibility...

Coppa Piacentina is an Italian protected designation of origin (PDO) dry-cured product obtained from the muscle pork neck and ripened for at least six months. Metabolomics- volatilomics-based strategies, combined with a chemical characterization free amino acids were applied to identify biomarkers long-ripened PDO. Long ripening induced significantly increase total acids, mainly represented by glutamic acid, involved in umami taste perception. Untargeted metabolomics, performed using...

A survey on the occurrence of ochratoxin (OTA) and sterigmatocystin (STC) in grated cheese products obtained from hard grana-type cheeses was carried out, where 107 were collected retail outlets analysed. OTA STC found 48.6% 94.4% samples, a range <LOD to 25.05 µg kg−1 6.87 kg−1, respectively. detected all OTA-contaminated samples. The is due environmental contamination during ripening, leading fungal growth mycotoxin production surface. This statement confirmed by analysing surface 16...

In recent years, the cultivation of hemp (Cannabis sativa L.) in Europe has aroused interest among farmers for potential market opportunities its products; increased from 20,450 ha 2015 to 33,020 2022. Thanks great versatility this crop, there are food and nutraceutical fields (gluten free), cosmetics, energy industrial sectors. As several crops, seeds may also be contaminated by fungal pathogens compromising quality safety. Considering consumers using purposes, present work, a small survey...

Abstract In a context where the commercial and nutritional interest in insect chitin is always increasing, an accurate precise method to quantify this biopolymer, especially food/feed, required. addition, quantification of crude protein through nitrogen determination normally overestimated due presence chitin. work, for first time, RP-UPLC-ESI/MS simultaneous insects chitin, as glucosamine (GlcN), protein, total amino acids, presented. The based on acid hydrolysis derivatization acids GlcN...

Nowadays, the mass production of insects is promoted not only for their nutritional value, but also ability to convert agri-food by-products into high valuable derived products. In present work, potential carotenoid-rich larvae black soldier fly, Hermetia illucens (BSFL), was evaluated by rearing them on different by-products. The carotenoid concentration in BSFL highly correlated with content substrates (R=0.882). Among substrates, tomato and carrot allowed obtain a total 230% higher than...

Ripening time is known to drive the chemical and sensory profiles of dry meat products, thus potentially affecting final quality product. Starting from these background conditions, aim this work was shed light, for first time, on modifications a typical Italian PDO product-namely, Coppa Piacentina-during ripening, find correlations between its biomarker compounds related progress ripening. The ripening (from 60 240 days) found deeply modify composition product, providing potential biomarkers...