A5004060857- Neuroendocrine regulation and behavior
- Neuropeptides and Animal Physiology
- Hypothalamic control of reproductive hormones
- Neuroscience of respiration and sleep
- Sexual function and dysfunction studies
- Circadian rhythm and melatonin
- Infant Health and Development
- Electrolyte and hormonal disorders
- Infant Development and Preterm Care
- Antimicrobial Peptides and Activities
- Urinary Bladder and Prostate Research
- Thyroid Disorders and Treatments
- Receptor Mechanisms and Signaling
- Pain Mechanisms and Treatments
- Stress Responses and Cortisol
- Neurobiology and Insect Physiology Research
- Ion Channels and Receptors
- Dermatology and Skin Diseases
- Ion channel regulation and function
- Toxic Organic Pollutants Impact
- Effects and risks of endocrine disrupting chemicals
- Pediatric Pain Management Techniques
- ATP Synthase and ATPases Research
- Hormonal and reproductive studies
- Chemical Synthesis and Analysis
Okayama University
2012-2023
Kanagawa University
2020-2023
Marine Institute
2015-2020
Kyushu University
2020
Okayama University of Science
2013
Gastrin-releasing peptide (GRP) has recently been identified as an itch-specific neuropeptide in the spinal sensory system mice, but there are no reports of expression and distribution GRP trigeminal mammals. We characterized compared GRP-immunoreactive (ir) neurons ganglion (TG) with those rat dorsal root (DRG). immunoreactivity was expressed 12% TG 6% DRG restricted to small- medium-sized type cells. In both DRG, many GRP-ir also substance P calcitonin gene-related peptide, not isolectin...
Arginine vasopressin (AVP) is a neurohypophysial hormone synthesized as part of prepropeptide precursor containing the signal peptide, AVP hormone, AVP‐associated neurophysin II and copeptin in hypothalamic neurosecretory neurons. A transgenic (Tg) rat line expressing AVP‐eGFP fusion gene has been generated. To establish Tg unique model for an analysis dynamics vivo , we first examined molecular gene, then release GFP response to physiological stimuli. Double immunoelectron microscopy...
Abstract Arginine vasopressin (AVP) is synthesized in parvocellular‐ and magnocellular neuroendocrine neurons the paraventricular nucleus (PVN) of hypothalamus. Whereas AVP project primarily to posterior pituitary, parvocellular median eminence (ME) extrahypothalamic areas. The gene encodes pre‐pro‐AVP that comprises signal peptide, AVP, neurophysin (NPII), a copeptin glycopeptide. In present study, we used an N‐terminal antiserum examine expression hypothalamus mouse, rat, macaque monkey....
In rats, a sexually dimorphic spinal gastrin-releasing peptide (GRP) system in the lumbosacral cord projects to centers that control erection and ejaculation. This controls sexual function of adult males an androgen-dependent manner. present study, we assessed influence androgen exposure on GRP during critical period development dimorphism. Immunohistochemistry was used determine if is regulated by perinatal surge. We first analyzed responses neonates administered with anti-androgen...
The spinal ejaculation generator (SEG) is located in the central gray (lamina X) of rat lumbar cord and plays a pivotal role ejaculatory reflex. We recently reported that SEG neurons express oxytocin receptor are activated by projections from paraventricular nucleus hypothalamus (PVH). However, it unknown whether responds to vivo. In this study, we analyzed characteristics brain–spinal neural circuit controls male sexual function using newly developed vivo electrophysiological technique....
Abstract Bombesin is a putative antibacterial peptide isolated from the skin of frog, Bombina bombina . Two related (bombesin-like) peptides, gastrin-releasing (GRP) and neuromedin B (NMB) have been found in mammals. The history GRP/bombesin discovery has caused little attention to be paid evolutionary relationship their receptors vertebrates. We classified peptides phylogenetic viewpoint using newly established genetic database bioinformatics. Here we show, by clawed frog ( Xenopus...
Several regions of the brain and spinal cord control male reproductive function. We previously demonstrated that gastrin-releasing peptide (GRP) system, located in lumbosacral rats, controls centers to promote penile reflexes during copulatory behavior. However, little information exists on male-specific GRP system animals other than rats. The objective this study was examine functional generality mammals using Asian house musk shrew (Suncus murinus; suncus named as laboratory strain), a...
A sexually dimorphic spinal gastrin-releasing peptide (GRP) system in the lumbosacral cord, which projects to lower centers, controls erection and ejaculation rats. However, little is known about postnatal development of this system. In study, we therefore examined male-dominant GRP its sexual differentiation rats using immunohistochemistry. Our results show that expression prominent from onset puberty dimorphism persists into adulthood. These suggest androgen surge during male plays an...
Abstract Exposure to endocrine‐disrupting chemicals may adversely affect animals, particularly during development. Tris(1,3‐dichloroisopropyl) phosphate (TDCIPP) is an organophosphate with anti‐androgen function in vitro that present indoor dust at relatively high concentrations. In male rats, androgens are necessary for the development of reproductive organs, as well endocrine and central nervous systems. However, we currently do not know exact effects TDCIPP exposure through suckling on...
Abstract Gastrin‐releasing peptide (GRP) and its receptor (GRPR) have been identified as itch mediators in the spinal trigeminal somatosensory systems rodents. In primates, there are few reports of GRP/GRPR expression or function sensory system virtually nothing is known system. The aim present study was to characterize GRP GRPR Japanese macaque monkeys ( Macaca fuscata ). cDNA encoding isolated from dorsal root ganglion (DRG) exhibited an amino acid sequence that highly conserved among...
The gastrin-releasing peptide (GRP) system in the lumbosacral spinal cord controls male sexual function rats. In contrast, female rats, GRP neurons could scarcely be detected around puberty when circulating ovarian steroid hormones such as estradiol and progesterone levels are increasing. However, little information is available on feminizing or demasculinizing effects of steroids central nervous adulthood. this study, to visualize vivo, we generated a GRP-promoter-Venus transgenic (Tg) rat...
Oxytocin is produced in the hypothalamus and stimulates uterine contraction milk ejection. While many people consider oxytocin to be a female hormone, it reported that, men, plasma level increases markedly after ejaculation. However, this aspect of physiology poorly understood. The spinal ejaculation generator (SEG), which expresses neuropeptide, gastrin-releasing peptide (GRP), can trigger rats. Therefore, we focused on systemic effects GRP/SEG neuron system lumbar cord controlling sexual...
Male sexual function in mammals is controlled by the brain neural circuits and spinal cord centers located lamina X of lumbar (L3-L4). Recently, we reported that hypothalamic oxytocin neurons project to activate dorsal (dXL) via receptors, thereby facilitating male activity. Sexual experiences can influence activity rats. However, how this experience affects brain-spinal underlying remains unknown. Focusing on dXL are innervated oxytocinergic controlling function, examined whether such...
Translational research often requires the testing of experimental therapies in primates, but non-human primates is now stringently controlled by law around world. Tissues fixed formaldehyde without glutaraldehyde have been thought to be inappropriate for use electron microscopic analysis, particularly those brain. Here we report immunoelectron characterization arginine vasopressin (AVP)-producing neurons macaque hypothalamo-pituitary axis tissues perfusion with 4% and stored at -25 °C...
Neural circuits underlying male sexual function comprise several nuclei located in the brain and spinal cord. We have previously demonstrated rats that gastrin-releasing peptide (GRP) system influences centers promoting penile reflexes. Moreover, a group of oxytocin (OXT) neurons, situated parvocellular part paraventricular nucleus hypothalamus, project into cord control Therefore, it has been hypothesized OXT is transported by long descending paraventriculospinal pathways activates...
Abstract Gastrin-releasing peptide (GRP), first isolated from the porcine stomach, is a neuropeptide that modulates autonomic system in mammals and has previously been considered to be mammalian equivalent of bombesin, fourteen amino acid skin European fire-bellied toad, Bombina bombina . Bombesin-like peptides related neuromedin B (NMB) have since identified mammals. However, orthologous relationships among GRP/NMB/bombesin their receptors vertebrates are still not well understood. Our...
There is considerable current interest concerning the role of neuropeptide oxytocin in regulation socio-sexual behaviors mammals, ranging from social bonding to empathy. However, precise mechanisms by which sparsely dispersed fibers central nervous system lead activation distributed receptor populations are unclear. Here we present evidence, rats, that release lumbar spinal cord not limited synapses, but occurs axonal varicosities and acts diffusion – a localized volume transmission reach...