A5049167152- Microbial Metabolism and Applications
- Biocrusts and Microbial Ecology
- Bacterial biofilms and quorum sensing
- Phytochemistry and Bioactivity Studies
- Algal biology and biofuel production
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Enzyme Production and Characterization
- Birth, Development, and Health
- Chromium effects and bioremediation
- Microbial Natural Products and Biosynthesis
- Microbial Community Ecology and Physiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Helicobacter pylori-related gastroenterology studies
- Trypanosoma species research and implications
- Gastroesophageal reflux and treatments
- Cassava research and cyanide
- Diet and metabolism studies
Universidade de São Paulo
2018-2025
Universidade de Ribeirão Preto
2019-2023
Faculdade de Medicina de São José do Rio Preto
2023
Associação Saúde da Família
2023
SUPERA Park of Innovation and Technology of Ribeirão Preto
2019
Clinics Hospital of Ribeirão Preto
2019
Universidade Federal do Triângulo Mineiro
2019
ABSTRACT Chromobacterium violaceum is a ubiquitous environmental pathogen. Despite its remarkable adaptability, little known about the mechanisms of stress resistance in this bacterium. Here, screen for iron-susceptible transposon mutants, we identified cytochrome bd that protects C. against multiple stresses. The two subunits (CioAB) are encoded by cioRAB operon, which also encodes GbsR-type MarR family transcription factor (CioR). A ∆ cioAB mutant strain was sensitive to iron and...
Iron is a transition metal used as cofactor in many biochemical reactions. In bacteria, iron homeostasis involves Fur-mediated de-repression of uptake systems, such the iron-chelating compounds siderophores. this work, we identified and characterized novel regulatory systems that control siderophores environmental opportunistic pathogen
Maintenance of iron homeostasis, i.e., avoiding both deficiency and toxicity this metal, is vital to bacteria their hosts. Iron sequestration by host proteins a crucial strategy combat bacterial infections. In bacteria, the ferric uptake regulator Fur coordinates expression several iron-related genes. Sometimes, can also regulate other processes. work, we performed an in-depth phenotypic characterization fur mutants in human opportunistic pathogen Chromobacterium violaceum . We determined...
Chromobacterium violaceum is an environmental Gram-negative beta-proteobacterium that causes systemic infections in humans. C. uses siderophore-based iron acquisition systems to overcome the host-imposed limitation, but its capacity use other sources unknown. In this work, we characterized ChuPRSTUV as a heme utilization system employed by explore important reservoir mammalian hosts, free and hemoproteins. We demonstrate chuPRSTUV genes comprise Fur-repressed operon expressed under...
Chromobacterium violaceum is an environmental Gram-negative bacterium that causes infections in humans. Treatment of C. difficult and little known about the mechanisms antibiotic resistance this bacterium. In work, we identified mutations MarR family transcription factor EmrR protein GyrA as key determinants quinolone violaceum, defined a repressor MFS-type efflux pump EmrCAB. Null deletion emrR caused increased to nalidixic acid, but not other quinolones or antibiotics different classes....
Bacteria use siderophores to scavenge iron from environmental or host sources. The acquisition systems of Chromobacterium violaceum, a ubiquitous bacterium that can cause infections in humans, are still unknown. In this work, we demonstrated C. violaceum produces putative distinct endogenous siderophores, here named chromobactin and viobactin, showed they each required for uptake virulence. An silico analysis the genome revealed genes related synthesis (cba) viobactin (vba) located within...
Trypanosoma cruzi and Helicobacter pylori (HP) are pathogens that cause chronic diseases have been associated with hypergastrinemia. The aim of this study was to evaluate the fasting gastrin levels in patients different clinical forms Chagas disease (CD), coinfected or not by HP. enrolled individuals were outpatients attending at university hospital. HP infection assessed serology 13 C-urea breath test. Fasting serum concentration measured chemiluminescence assay. Gastric endoscopic...
is a ubiquitous environmental pathogen. Despite its remarkable adaptability, little known about the mechanisms of stress resistance in this bacterium. Here, screen for iron-susceptible transposon mutants, we identified cytochrome
ABSTRACT Bacterial resistance to beta-lactam antibiotics mediated by beta-lactamase enzymes is widespread worldwide. Chromobacterium violaceum , an environmental Gram-negative bacteria pathogen, intrinsically resistant some antibiotics. In this work, we found that mutations in ampD gene, encoding a peptidoglycan-recycling amidase, cause hyperproduction of two chromosomal beta-lactamases (AmpC and CphA), conferring high C. . Susceptibility tests using Δ ampC cphA ΔampC mutant strains revealed...
Abstract Iron is a transition metal used as cofactor in many biochemical reactions. In bacteria, iron homeostasis involves Fur-mediated de-repression of uptake systems, such the iron-chelating compounds siderophores. this work, we identified and characterized novel regulatory systems that control siderophores environmental opportunistic pathogen Chromobacterium violaceum . Screening 10,000-transposon mutant library for siderophore halos on PSA-CAS plates seven possible involved...
ABSTRACT Chromobacterium violaceum is an environmental Gram-negative beta-proteobacterium that causes systemic infections in humans. C. uses siderophore-based iron acquisition systems to overcome the host-imposed limitation, but its capacity use other sources unknown. In this work, we characterized ChuPRSTUV as a heme utilization system employed by explore important reservoir mammalian hosts, free and hemoproteins. We demonstrate chuPRSTUV genes comprise Fur-repressed operon expressed under...