A5037909723- Milk Quality and Mastitis in Dairy Cows
- Probiotics and Fermented Foods
- Food Safety and Hygiene
- Salmonella and Campylobacter epidemiology
- Microbial infections and disease research
- Listeria monocytogenes in Food Safety
- Identification and Quantification in Food
- Meat and Animal Product Quality
- Proteins in Food Systems
- Digestive system and related health
- Infant Nutrition and Health
- Agricultural and Food Sciences
- Antimicrobial Resistance in Staphylococcus
- Microbial Inactivation Methods
- Biopolymer Synthesis and Applications
- Sensory Analysis and Statistical Methods
- T-cell and B-cell Immunology
- Rural Development and Agriculture
- Pharmaceutical and Antibiotic Environmental Impacts
- Poxvirus research and outbreaks
- Protein Hydrolysis and Bioactive Peptides
- Animal Diversity and Health Studies
- Enterobacteriaceae and Cronobacter Research
- Pesticide Residue Analysis and Safety
- Immunotherapy and Immune Responses
Universidade Federal de Minas Gerais
2013-2022
Prefeitura Municipal de Belo Horizonte
2017
Ezequiel Dias Foundation
2017
Universidade Federal de Viçosa
2016
Universidade Estadual Paulista (Unesp)
2016
The purpose of this study was to identify enterotoxin genes from isolates coagulase-negative staphylococci and coagulase-positive obtained dairy products, responsible for 16 outbreaks food poisoning.From the pool 152 staphylococcal isolates, 15 representatives were selected study. tested presence coa femA genes, which are known be characteristic Staphylococcus aureus. After testing by polymerase chain reaction (PCR), 30 toxin immunoassay.Seven amplified gene subsequently reclassified as...
Abstract: The most acceptable criteria for diagnosing bovine intramammary infections include results of bacteriological culture and measures inflammation. Therefore, information on the diagnostic characteristics procedures used to identify infected quarters is required. Thus, this study was designed evaluate a set classify infectious status an udder at quarter (single duplicate milk samples) cow (composite sample) levels, compare with somatic cell counts (SCCs) samples. Here, SCC thresholds...
ABSTRACT The influence of seasonality and the training implementation good dairy farming practices on raw milk production quality was evaluated farms in Minas Gerais State, Brazil. physico-chemical composition, somatic cell count (SCC) total bacterial (TBC) were determined 3,096 samples collected from bulk tanks originated 43 over a three-year period correlated with key climatic factors. recommended milking management applied through program seasonal data three stages: I) prior to (dry...
Em 10 fazendas da região Serra do Salitre, MG, foram coletadas amostras de leite, soro fermentado (pingo), coalhada e queijo frescal para avaliar a microbiota bactérias láticas leveduras presentes. Uma diversidade menor foi observada durante produção quando comparada à leveduras. As espécies mais freqüentes Lactococcus lactis, Enterococcus spp., faecalis Streptococcus agalactiae Debaryomyces hansenii Kluyveromyces lactis. Apenas as populações Leuconostoc mesenteroides apresentaram aumento...
A indústria leiteira, visando a aumentar o rendimento industrial de seus produtos e melhorar qualidade desses para atender à demanda do mercado consumidor, tem sido cada vez mais exigente com fornecedores matéria-prima. Supõe-se que tipo ordenha metodologias coleta influenciam composição físico-química contagem células somáticas leite cru, tornando-se necessário verificar tais efeitos. Com objetivo comprovar essa hipótese, foram analisadas 180 amostras 30 vacas. Utilizou-se arranjo fatorial...
Bovine vaccinia is an emergent zoonosis caused by the Vaccinia virus (VACV). The disease characterized appearance of exanthematic lesions that occur in humans and dairy cows. Previous studies have revealed presence infectious viral particles milk samples during outbreak bovine Brazil, indicating possibility transmission through raw milk. To assess viability after thermal treatment processing procedures, were experimentally contaminated with 103 plaque forming units (PFU)/mL (group I) 105...
Streptococcus dysgalactiae is a bacterium that accounts for notable proportion of both clinical and subclinical intramammary infections (IMIs). Thus, the present study explores function milk neutrophils lymphocyte profile in mammary glands naturally infected with . Here, we used 32 culture-negative control quarters from eight clinically healthy dairy cows low somatic cell counts 13 S. -infected six cows. Using flow cytometry, evaluated percentage monocytes/macrophages neutrophils, expression...
In the present study, we aimed to determine antimicrobial resistance and molecular typing of Staphylococcus aureus recovered from transient persistent intramammary infections nares/muzzles in dairy cows. We investigated 189 S. strains using a broad susceptibility profile. Furthermore, 107 isolates were strain-typed staphylococcal protein-A (spa) typing. A large proportion exhibited multidrug antimicrobials, including critically important although no methicillin-resistant found. Our study did...
Analisaram-se 80 amostras de leite cru refrigerado a 4°C e estocado por 48 horas em tanques refrigeradores propriedades rurais do estado Minas Gerais quanto à contagem identificação Staphylococcus sp. detecção enterotoxinas estafilocócicas (SE) da toxina síndrome choque tóxico (TSST-1). foi detectado 100% das tanque refrigerador contagens que variaram 1,0 × 10(5) 2,5 10(7) UFC/ml (média = 5,60 log UFC/ml; s 0,53 CV 9,5%). Isolaram-se identificaram-se 436 estirpes como: S. aureus, S hyicus,...
Analisou-se a evolução anual da qualidade de leite cru refrigerado pelo levantamento e análise estatística do banco dados das análises individuais dos tanques refrigeradores, computadas mensalmente entre abril 2002 dezembro 2008 em uma indústria Minas Gerais. O volume conformidade com IN51 para contagem bacteriana total o ano aumentou 74,3% 85,8% 2008. Considerando-se 2011, esses valores seriam 30,5% 46,6%, respectivamente. células somáticas reduziu-se 93,5% 90,1% Para 64,9 60,5%, Em relação...
Bovine vaccinia (BV) is a zoonosis caused by Vaccinia virus (VACV), which affects lactating cows and milkers. VACV DNA infectious particles have been detected in milk of naturally infected cows. However, the period pattern shedding unknown, as whether presence due to localized or systemic infection. To address those questions, eight were inoculated with previously scarified teats. The experiment was divided two phases. In Phase 1, samples collected daily for 33 days, 2, four animals from...
Mastitis affects a high proportion of dairy cows and is still one the greatest challenges faced by industry. Staphylococcal bacteria remain most important cause mastitis worldwide. We investigated how distinct staphylococcal species evade some critical host defense mechanisms, which may dictate establishment, severity, persistence infection outcome possible therapeutic prevention interventions. Thus, present study variations among bovine-associated staphylococci in their capability to resist...
The antimicrobial resistance profile of 45 Staphylococcus strains isolated from 10 samples Brazilian type coalho cheese was evaluated against eight antibiotics used either in human or veterinarian medicine. tested decreasing degree was: penicillin (100.0%), tetracycline (91.0%), vancomycin (75.5%), gentamicin (71.1%), oxacillin (66.7%), erythromycin (60.0%), cephalothin (48.9%) and sulphazothrin (26.7%). high frequency presenting to the antibiotics, emphasizes importance control abusive use...
Vaccinia virus (VACV) is the agent of bovine vaccinia (BV), an emerging zoonosis that causes exanthematic lesions on teats dairy cows and hands milkers. The has been detected in milk naturally infected cows. objective this study was to investigate quantify VACV DNA as well presence infectious particles samples cheese curd, whey pasteurized produced using from experimentally inoculated with VACV-GP2, a Brazilian isolate (VACV-BR). at different time points, even after resolution typical caused...
The objective of this study was to evaluate the CombiScope FTIR equipment based on Fourier Transform Infrared methodology (FTIR), assess content milk urea nitrogen (MUN) in Brazil. Repeatability and reproducibility CombiScopeTM (Delta Instruments), comparison with an enzymatic automated method (Chemspec® 150; Bentley Instruments) were tested measure raw (MUN). Additionally, MUN levels stability after storage samples at 4ºC, 20ºC for up 15 days, capability precision detect extraneous added as...
ABSTRACT A total of 480 milk samples were analyzed in four repetitions with preservative treatments (no preservative, Bronopol, Bronolat and Brononata), three storage times at temperatures up to 4 °C (24, 48 72hours after reception), five different water additions (0.0, 2.5, 5.0, 7.5 10.0%) two analytical instruments (electronic cryoscope FTIR). The objective this study was evaluate the effect these parameters determination freezing point by reference method Fourier transform infrared...