A5001840023- Toxoplasma gondii Research Studies
- Bacterial biofilms and quorum sensing
- Leptospirosis research and findings
- Herpesvirus Infections and Treatments
- Milk Quality and Mastitis in Dairy Cows
- Parasitic Infections and Diagnostics
- Antibiotic Resistance in Bacteria
- Antimicrobial Resistance in Staphylococcus
- Mycobacterium research and diagnosis
- Analytical chemistry methods development
- Antimicrobial Peptides and Activities
- Cytomegalovirus and herpesvirus research
- Rabies epidemiology and control
- Vibrio bacteria research studies
- Animal Nutrition and Physiology
- Comparative Animal Anatomy Studies
- Microbial Metabolism and Applications
- Gut microbiota and health
- Antimicrobial agents and applications
- Probiotics and Fermented Foods
- Microbial infections and disease research
- Bird parasitology and diseases
- Veterinary medicine and infectious diseases
- Invertebrate Immune Response Mechanisms
- Escherichia coli research studies
Universidade de São Paulo
2013-2024
University of Antwerp
2019-2023
Background Chronic airway inflammation is the main driver of pathogenesis in respiratory diseases such as severe asthma, chronic obstructive pulmonary disease, cystic fibrosis (CF) and bronchiectasis. While role common pathogens widely recognised, influence other microbiota members still poorly understood. Methods We hypothesised that lung contains bacteria with immunomodulatory activity which modulate net levels immune activation by key pathogens. Therefore, we assessed effect several...
Streptococcus pneumoniae is the leading cause of bacterial pneumonia. Infection linked to high morbidity and mortality rates antibiotic resistance within this pathogen on rise. Therefore, there a need for novel antimicrobial therapies. To lower time costs drug discovery process, alternative in vivo models should be considered. As such, Galleria mellonella larvae can great value. The larval immunity consisting several types haemocytes remarkably similar human innate immune system....
Abstract Microtiter plate methods are commonly used for biofilm assessment. However, results obtained with these have often been difficult to reproduce. Hence, it is important obtain a better understanding of the repeatability and reproducibility methods. An interlaboratory study was performed in five different laboratories evaluate responsiveness three quantify Staphylococcus aureus formation 96-well microtiter plates: crystal violet, resazurin, counts. inter-lab protocol developed study....
Clinicians are increasingly confronted with the limitations of antibiotics to clear bacterial infections in patients. It has long been assumed that only antibiotic resistance plays a pivotal role this phenomenon. Indeed, worldwide emergence is considered one major health threats 21st century. However, presence persister cells also significant influence on treatment outcomes. These antibiotic-tolerant present every population and result phenotypic switching normal, antibiotic-sensitive cells....
In Brazil, studies on animals and humans in mainland areas have shown that most strains of Toxoplasma gondii are pathogenic to mice exhibit great genetic variability. this study, using a set 11 PCR-RFLP 15 microsatellite markers, we isolated genetically characterised T. from one cat three rats Fernando de Noronha Island. The had antibodies gondii, which were revealed modified agglutination test (MAT, cut-off 1:25) the seroprevalence among 46 rodents was 15.2%. Viable (TgCatBrFN1), two brown...
In the present study, we aimed to determine antimicrobial resistance and molecular typing of Staphylococcus aureus recovered from transient persistent intramammary infections nares/muzzles in dairy cows. We investigated 189 S. strains using a broad susceptibility profile. Furthermore, 107 isolates were strain-typed staphylococcal protein-A (spa) typing. A large proportion exhibited multidrug antimicrobials, including critically important although no methicillin-resistant found. Our study did...
Abstract The aims of this study were to determine the antimicrobial susceptibility profile and genetic diversity Staphylococcus spp. isolated from dairy cows in Minas Gerais, Brazil, assess relationship among isolates’ profiles pulsed‐field gel electrophoresis ( PFGE ) genotypes. Seventy‐nine isolates used, including S. aureus n = 71) coagulase‐negative staphylococci (Co NS 8). Susceptibility 12 agents was performed. All subjected . Co exhibited full only cephalothin. greatest percentages...
Leptospirosis is a disease of worldwide distribution that affects man and several animal species. Domestic wild animals can behave as reservoirs the agent. The aim this study was to evaluate frequency anti-Leptospira spp. antibodies in dogs small mammals from rural properties conservation units three municipalities Rio Grande do Sul State, Brazil. Sera were collected 192 domestic 132 mammals, namely rodents Cricetidae Caviidae families marsupials Didelphidae family. used Microscopic...
Abstract Recent genetic population studies on Toxoplasma gondii in Brazil have shown large variability. The objective of the present study was to isolate and genotypically characterize T. from free-ranging captive wild mammals birds Pernambuco state, Brazil. Fragments heart, brain, skeletal muscle diaphragm tissue 71 34 mammals, which were either or captive, collected. Samples 32 these animals subjected bioassays mice. remaining 73 underwent biomolecular diagnosis, using PCR technique,...
Abstract Capybaras (Hydrochoerus hydrochaeris) are the largest rodents found in South America. The aim of present study was to investigate occurrence anti-Toxoplasma gondii and anti-Neospora caninum antibodies 170 free-living capybaras a residential park area Itu Municipality, São Paulo State, Brazil. Serum samples were tested by indirect fluorescent antibody test (IFAT) for T. (IFAT ≥ 1:16) N. 1:50). Among analyzed, 10% (17/170) 0% (0/170) seropositive caninum, respectively. This confirms...
The isolation of Leptospira is challenging, since the bacteria this genus are susceptible to adverse environmental conditions and may not remain viable for extend periods in urine samples. This study attempted develop evaluate a simple practical method isolate leptospires from bovine A culture medium sample transport, named Transport Medium (LTM), was described validated using reference serovars spp. addition autochthonous strains isolated Brazil. We evaluated LTM field, by collecting 215...
Additive manufacturing is widely used to produce highly complex structures. Moreover, this technology has proven its superiority in producing tools which can be different applications. We designed and produced an extrusion nozzle that allowed us hot melt extrude drug-loaded tubes. The tubes were essential part of a new mouse ventilator-associated pneumonia (VAP) model. Ciprofloxacin (CPX) was selected for expected activity against the pathogen Staphylococcus aureus ease incorporation into...
Morphological studies of the gastrointestinal tract blue-and-yellow macaws (Ara ararauna) are scarce. In view paucity information regarding digestive macaws, this study aims to describe gross anatomical features (oesophagus cloaca) as part a broad (GIT) these birds. Three animals (two males and one female) adult were anatomically dissected from oropharynx cloaca expose GIT. The oesophagus was identified muscle-membranous tube continuous with crop, which intimately attached skin. internal...
SUMMARY The biological and genetic diversity of Neospora caninum is very limited because availability only a few viable isolates worldwide. This study describes the isolation molecular characterization new isolate N. (NC-SP1), from cattle in Brazil. Approximately 400 g brain naturally infected adult male an abattoir was fed to 2-month-old dog. -like oocysts were observed on day 7 post-inoculation (PI) duration oocyst shedding 14 days. DNA obtained characterized molecularly final sequence 99%...
In the present study, we aimed to determine antimicrobial resistance and genetic structure of a population S. aureus recovered from transient persistent intramammary infections nares/muzzles. We investigated 189 strains using broad susceptibility profile. Furthermore, 107 isolates were strain-typed staphylococcal protein-A (spa) typing. Here, great proportion exhibited multidrug antimicrobials, including critically important although no methicillin-resistant found. Our study did not...
Previous studies have reported high diversity between and within populations of Toxoplasma gondii in South America. In the present study, isolates T. from chickens were obtained Amazon region. Adult free-range acquired 29 municipalities Brazilian region that included Acre (n = 9 municipalities), Amapá 6), Amazonas Pará Roraima 2) states two Peru, three Bolivia, one Guyana, Venezuela. Heart, brain, blood samples collected 401 chickens. Anti- serum antibodies detected 273 (68.1%) using...
O potencial zoonótico de bactérias multirresistentes é uma preocupação global e os animais companhia têm sido implicados na disseminação resistentes; assim, importante a vigilância, pois já existem relatos transmissão destas do cão para o homem vice-versa. Um cão, sem raça definida cinco anos idade, foi atendido cidade Botucatu, São Paulo, Brasil, apresentando urolitíase obstrutiva estruvita recorrente há um ano meio. Na urocultura animal isolada estirpe Staphylococcus spp. multirresistente;...
Changes were made to the original formulation of EMJH medium (Ellinghausen–McCullough–Johnson–Harris) enrichment and some aspects such as growth time Leptospira utilization in microscopic agglutination test (MAT) evaluated compared a commercially available (DIFCO™). samples (24 antigens) that make up our panel antigens used MAT used, among them, reference autochthonous strains isolated Brazil. The grown individually under three previously mentioned conditions (adapted enrichment, commercial...
Abstract Nowadays, clinicians are more and confronted with the limitations of antibiotics to completely cure bacterial infections in patients. It has long been assumed that only antibiotic resistance plays a pivotal role this. Indeed, worldwide emergence is considered as one major health threats 21 st century. However, presence persister cells also significant influence on treatment outcomes. These antibiotic-tolerant present every population result phenotypic switching normal,...