A5013080582- Bacterial biofilms and quorum sensing
- Antimicrobial Peptides and Activities
- Cholinesterase and Neurodegenerative Diseases
- Computational Drug Discovery Methods
- Biochemical and Structural Characterization
- Chemical synthesis and alkaloids
- Bacteriophages and microbial interactions
- Antimicrobial Resistance in Staphylococcus
- Microbial Natural Products and Biosynthesis
- Antimicrobial agents and applications
- Seaweed-derived Bioactive Compounds
- Microbial Community Ecology and Physiology
- Bioactive Compounds and Antitumor Agents
- Biosensors and Analytical Detection
- Alzheimer's disease research and treatments
- Analytical chemistry methods development
- Biological Activity of Diterpenoids and Biflavonoids
- Synthesis and biological activity
- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Probiotics and Fermented Foods
- Venomous Animal Envenomation and Studies
- Plant-based Medicinal Research
- Oral microbiology and periodontitis research
- Orthopedic Infections and Treatments
University of Helsinki
2014-2023
Thermo Fisher Scientific (Finland)
2022
University of Havana
2000-2015
Åbo Akademi University
2006-2015
University of Eastern Finland
2000
When single-cell (or suspended) bacteria switch into the biofilm lifestyle, they become less susceptible to antimicrobials, imposing need for anti-biofilms research. Flavonoids are among most extensively studied natural compounds with an unprecedented amount of bioactivity claims. Most studies focus on antibacterial effects against suspended cells; fewer reports have researched their anti-biofilm properties. Here, a high throughput phenotypic platform was utilized screen inhibitory activity...
The lack of reproducibility published studies is one the major issues facing scientific community, and field biofilm microbiology has been no exception. One effective strategy against this multifaceted problem use minimum information guidelines. This provides a guide for authors reviewers on necessary that manuscript should include experiments in study to be clearly interpreted independently reproduced. As result several discussions between international groups working area biofilms, we...
Owing to the failure of conventional antibiotics in biofilm control, alternative approaches are urgently needed. Inhibition quorum sensing (QS) represents an attractive target since it is involved several processes essential for formation. In this study, a compound library natural product derivatives (n = 3040) was screened anti-quorum activity using Chromobacterium violaceum as reporter bacteria. Screening assays, based on QS-mediated violacein production and viability, were performed...
Flavonoids are known for their beneficial effects on human health, and therefore the therapeutic potential of these compounds have been extensively studied. Flavone has previously identified as a tankyrase inhibitor, to further elucidate whether tankyrases would be inhibited by other flavonoids, we performed systematic screening 2 inhibitory activity using 500 natural naturally derived flavonoids covering nine different flavonoid classes. All inhibitors were flavones. We report crystal...
Potent drugs are desperately needed to counteract bacterial biofilm infections, especially those caused by gram-positive organisms, such as Staphylococcus aureus. Moreover, anti-biofilm compounds/agents that can be used chemical tools also for basic in vitro or vivo studies aimed at exploring biofilms behavior and functionability. In this contribution, a collection of naturally-occurring abietane-type diterpenes their derivatives was tested against S. aureus using platform consisting two...
Abstract Biofilm formation leads to the failure of antimicrobial therapy. Thus, biofilm prevention is a desirable goal research. In this study, efficacy antibiotics (doxycycline, oxacillin and rifampicin) in preventing Staphylococcus aureus biofilms was investigated using Microtiter Well Plates (MWP) Drip Flow Reactors (DFR), two models characterized by absence presence continuous flow nutrients, respectively. Planktonic culture S. exposed for one hour followed 24 hours incubation with fresh...
Background: Efficient localized cervicovaginal antibacterial therapy, enabling the delivery of antibiotic to site action at lower doses while escaping systemic drug effects and reducing risk developing microbial resistance, is attracting considerable attention. Liposomes have been shown allow sustained release into vaginal mucosa improve antibiotics bacterial cells biofilms. Azithromycin (AZI), a potent broad-spectrum macrolide antibiotic, has not yet investigated for therapy infections,...
Abstract Microtiter plate methods are commonly used for biofilm assessment. However, results obtained with these have often been difficult to reproduce. Hence, it is important obtain a better understanding of the repeatability and reproducibility methods. An interlaboratory study was performed in five different laboratories evaluate responsiveness three quantify Staphylococcus aureus formation 96-well microtiter plates: crystal violet, resazurin, counts. inter-lab protocol developed study....
Tankyrase, a member of human PARP protein superfamily, catalyzes covalent post-translational modification substrate proteins. This modification, poly(ADP-ribos)ylation, leads to changes in interactions and modifies downstream signaling events. Tankyrase 1 is potential drug target due its functions telomere homeostasis Wnt signaling. We describe here optimization application an activity-based homogenous assay for tankyrase inhibitors high-throughput screening format. The method measures the...
Streptomyces bacteria are renowned for their ability to produce bioactive secondary metabolites. Recently, synthetic biology has enabled the production of intermediates and shunt products, which may have altered biological activities compared end products pathways. Here, we evaluated potential recently isolated alnumycins other closely related pyranonaphthoquinone (PNQ) polyketides against Staphylococcus aureus biofilms. The antimicrobial potency compounds planktonic cells biofilms was...
Bacterial biofilms have clear implications in disease and food applications involving probiotics. Here, we show that switching the carbohydrate source from glucose to fructose increased biofilm formation total surface-antigenicity of a well-known probiotic,
Previously, we have proposed mupirocin-in-liposomes-in-hydrogel delivery system as advanced with the potential in treatment of burns. In current studies, evaluated for its cytotoxicity, ability to prevent biofilm formation, act on mature biofilms, and finally determined wound vivo mice burn model. The was found be nontoxic against HaCaT cells, that is, keratinocytes. It safe use exhibited antibiofilm activity S. aureus although more significant planktonic bacteria prior formation than...