A5083503187- Adenosine and Purinergic Signaling
- Radiopharmaceutical Chemistry and Applications
- Immune Response and Inflammation
- Cancer Mechanisms and Therapy
- Cancer, Hypoxia, and Metabolism
- Peptidase Inhibition and Analysis
- Phytochemical compounds biological activities
- Nanoplatforms for cancer theranostics
- Bone health and treatments
- Fibroblast Growth Factor Research
- Probiotics and Fermented Foods
- Cell Adhesion Molecules Research
- Lung Cancer Research Studies
- Neonatal Respiratory Health Research
- Cytomegalovirus and herpesvirus research
- Advanced biosensing and bioanalysis techniques
- Bone and Joint Diseases
- RNA Interference and Gene Delivery
- Bacteriophages and microbial interactions
- Pneumocystis jirovecii pneumonia detection and treatment
- Antimicrobial Peptides and Activities
- NF-κB Signaling Pathways
- Neuroendocrine Tumor Research Advances
- Retinal Diseases and Treatments
- Cancer Immunotherapy and Biomarkers
University of Turku
2016-2025
Åbo Akademi University
2016-2025
Turku Centre for Computer Science
2020-2025
Turku Centre for Biotechnology
2006-2022
University of Alabama at Birmingham
2011-2016
University of Oulu
2011
Oulu University Hospital
2011
The immune system plays a major role in cancer progression. In solid tumors, 5-40 % of the tumor mass consists tumor-associated macrophages (TAMs) and there is usually correlation between number TAMs poor prognosis, depending on type. resemble M2 macrophages. Unlike M1-macrophages which have pro-inflammatory anti-cancer functions, M2-macrophages are immunosuppressive, contribute to matrix-remodeling, hence favor growth. not fully understood breast Macrophage infiltration (CD68) activation...
Constitutive expression of the Pim-1 kinase prolongs survival cytokine-deprived FDCP1 cells, partly via maintenance Bcl-2 expression. Here, we show that colocalizes and physically interacts with pro-apoptotic Bad protein phosphorylates it in vitro on serine 112, which is a gatekeeper site for its inactivation. Furthermore, wild-type Pim-1, but not kinase-deficient mutant, enhances phosphorylation this cells protects from effects Bad. Our results suggest by one several mechanisms can enhance...
CD73 is a cell surface ecto-5'-nucleotidase, which converts extracellular adenosine monophosphate to adenosine. High tumor expression associated with poor outcome among triple-negative breast cancer (TNBC) patients. Here we investigated the mechanisms by might contribute TNBC progression. This was done inhibiting 5'-(α, β-methylene) diphosphate (APCP) in MDA-MB-231 or 4T1 cells through shRNA-silencing (sh-CD73). Effects of such inhibition on behavior then studied normoxia and hypoxia vitro...
Abstract Pim-1 is an oncogenic serine/threonine kinase implicated in cytokine-induced signal transduction and development of lymphoid malignancies. However, its precise function as well physiological substrates have remained unknown. In this study we demonstrate that can physically interact with the NFATc1 transcription factor phosphorylate it vitro on several serine residues. contrast to previously recognized NFATc kinases, wild-type enhances NFATc-dependent transactivation IL-2 production...
Abstract Astrocytes and microglia are able to degrade potentially neurotoxic β‐amyloid (Aβ) deposits typical for Alzheimer's disease (AD) pathology. Contrary microglia, astrocytes human Aβ from tissue sections in vitro without any additional stimulation, but it has remained unclear whether transplanted clear deposited vivo . We adult mouse into the hippocampi of transgenic mice mimicking AD observed their fate, effects on microglial responses, clearance. After 2‐months follow‐up time, we...
Abstract The aim of this study was to evaluate glass–fiber‐reinforced composite as a bone reconstruction material in the critical size defects rabbit calvarial bones. defect healing process and inflammatory reactions were evaluated histologically at 4 12 weeks postoperatively. Possible neuropathological effects on brain tissue evaluated. release residual monomers from fiber‐reinforced (FRC) analyzed by high performance liquid chromatograph (HPLC). Results: At postoperatively, fibrous...
Pim-1 is an oncogenic serine/threonine kinase with poorly defined function in epithelial cancers. In this study, we determined 1) associations of expression clinicopathological parameters including responsiveness to irradiation squamocellular cancers head and neck 2) how controlled subsequent irradiation. Moderate high correlated poor response radiation therapy (P = .003). It also associated the epidermal growth factor receptor (EGFR, P < .0001), which has been shown be activated by...
Abstract Background Pim family kinases are small constitutively active serine/threonine-specific kinases, elevated levels of which have been detected in human hematopoietic malignancies as well solid tumours. While we and others previously shown that the oncogenic stimulate survival cells, now examined their putative role regulating motility adherent cancer cells. For this purpose, inhibited kinase activity using a molecule compound, 1,10-dihydropyrrolo[2,3- ]carbazole-3-carbaldehyde...
Bacterial biofilms have clear implications in disease and food applications involving probiotics. Here, we show that switching the carbohydrate source from glucose to fructose increased biofilm formation total surface-antigenicity of a well-known probiotic,
Purpose. Increased CD73 expression has been associated with progression in various cancer types. Results of the AZURE and other trials suggest that, postmenopausal breast patients, adjuvant bisphosphonates inhibit bone relapses prolong overall survival. Based on these findings, (typically zoledronic acid) are standard-of-care patients high-risk early cancer. However, biomarkers needed for improved patient selection. The aim this study was to investigate association primary tumor later...
Abstract Background The pim family genes encode oncogenic serine/threonine kinases which in hematopoietic cells have been implicated cytokine-dependent signaling as well lymphomagenesis, especially cooperation with other oncogenes such myc , bcl -2 or Runx genes. α-subunits of heterodimeric transcription factors regulate cell proliferation and differentiation various tissues during development can become leukemogenic upon aberrant expression. Results Here we identified novel protein-protein...
ABSTRACT Plate counting and four culture-independent flow cytometric assays were used to determine the viability intrinsic properties of three probiotic strains during storage. The showed reduction in plate counts but able maintain esterase activity, intact cytoplasmic membrane, pH gradient. apparently uncultivable cells active stress resistant.
Zoledronic acid (ZOL) is a nitrogen-containing bisphosphonate used for the treatment of bone diseases and calcium metabolism. Anticancer activity ZOL has been established, but its extraskeletal effects are limited due to rapid uptake accumulation hydroxyapatite. In this work, we report on development tethered lipid bilayer-gated mesoporous silica nanocarriers (MSNs) incorporation, retention, intracellular delivery ZOL. The in vitro anticancer ZOL-loaded was evaluated by cell viability assay...
Accurate analysis and quantification of different testicular cell populations are central importance in studies male reproductive biology. The traditional histomorphometric immunohistochemical methods remain the gold standard studying complex dynamics tissue. Through past years advances have been made application flow cytometry for rapid populations. Detection DNA content surface antigens fluorescent reporters widely used to analyze sort cells. intracellular can broaden possibilities...
Toll-like receptor-9 (TLR9) is a cellular DNA sensor of the innate immune system. TLR9 widely expressed in number tumors, including brain cancer; however, little known regarding its regulation and involvement cancer pathophysiology. The present study demonstrated that hypoxia upregulates downregulates expression human cells vitro, cell-specific manner. In addition, hypoxia-induced upregulation was associated with invasion; such invasion not detected where had suppressed expression....
We recently reported N4-substituted 3-methylcytidine-5′-α,β-methylenediphosphates as CD73 inhibitors, potentially useful in cancer immunotherapy. now expand the structure–activity relationship of pyrimidine nucleotides human inhibitors. 4-Chloro (MRS4598 16; Ki = 0.673 nM) and 4-iodo (MRS4620 18; 0.436 substitution N4-benzyloxy group decreased by ∼20-fold. Primary alkylamine derivatives coupled through a p-amido with varying methylene chain length (24 25) were functionalized congeners, for...
Abstract Ecto-5’-nucleotidase (CD73) is a potential new drug target for cancer immunotherapy. Its overexpression associated with various aggressive cancers, including triple-negative breast (TNBC) and pancreatic cancer, making it promising diagnostic imaging. Besides antibodies, small molecule CD73 inhibitors have been developed are currently in clinical trials. This study aimed to develop evaluate fluorine-18 labeled high-affinity as tracers the non-invasive positron emission tomography...