A5054013035- Cancer Mechanisms and Therapy
- Chromatin Remodeling and Cancer
- Reproductive System and Pregnancy
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Cellular transport and secretion
- Viral gastroenteritis research and epidemiology
- RNA and protein synthesis mechanisms
- Ovarian cancer diagnosis and treatment
- Sepsis Diagnosis and Treatment
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Endoplasmic Reticulum Stress and Disease
- Respiratory viral infections research
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- SARS-CoV-2 and COVID-19 Research
- Fungal and yeast genetics research
Van Andel Institute
2019-2022
Genomics (United Kingdom)
2020
Georgetown University
2002
ARID1A and PI3-Kinase (PI3K) pathway alterations are common in neoplasms originating from the uterine endometrium. Here we show that monoallelic loss of mouse endometrial epithelium is sufficient for vaginal bleeding when combined with PI3K activation. Sorted mutant epithelial cells display gene expression promoter chromatin signatures associated epithelial-to-mesenchymal transition (EMT). We further bound to promoters open chromatin, but leads increased accessibility EMT genes. activation...
Endometriosis affects 1 in 10 women and is characterized by the presence of abnormal endometrium at ectopic sites. ARID1A mutations are observed deeply invasive forms disease, often correlating with malignancy. To identify epigenetic dependencies driving invasion, we use an unbiased approach to map chromatin state transitions accompanying loss endometrium. We show that super-enhancers marked high H3K27 acetylation strongly associated binding. leads hyperacetylation increased accessibility...
Precision medicine requires the translation of basic biological understanding to medical insights, mainly applied characterization each unique patient. In many clinical settings, this tools that can be broadly used identify pathology and risks. Patients often present intensive care unit with broad phenotypes, including multiple organ dysfunction syndrome (MODS) resulting from infection, trauma, or other disease processes. Etiology outcomes are individuals, making it difficult cohort patients...
Abstract To examine the functions of Arf‐like protein, Arl1p, in Saccharomyces cerevisiae , a null allele, arl1 Δ ::HIS3 was constructed two strains. In one background only, loss ARL1 resulted temperature‐sensitive (ts) growth (suppressed on high‐osmolarity media). Allelic variation at SSD1 locus accounted for differences between Strains lacking exhibited several defects membrane traffic. First, strains secreted less protein as measured by TCA‐precipitable radioactivity found media [ 35 S...
Abstract We present Single-cell TOtal RNA Miniaturized sequencing (STORM-seq), a full-length single-cell ribo-reduced protocol, optimized to profile thousands of cells per run. Using off-the-shelf reagents and random hexamer priming, STORM-seq recovers comprehensive profiles from single with library complexity approaching that bulk RNA-seq. Importantly, does not require specialized equipment can be performed using standard lab equipment. identifies additional coding non-coding transcripts...
Abstract Precision medicine requires the translation of basic biological understanding to medical insights, mainly applied characterization each unique patient. In many clinical settings, this tools that can be broadly used identify pathology and risks. Patients often present intensive care unit with broad phenotypes, including multiple organ dysfunction syndrome (MODS) resulting from infection, trauma, or other disease processes. Etiology outcomes are individuals, making it difficult cohort...