- Proteins in Food Systems
- Enzyme Catalysis and Immobilization
- Meat and Animal Product Quality
- Microbial Inactivation Methods
- Microencapsulation and Drying Processes
- Enzyme Production and Characterization
- Protein Hydrolysis and Bioactive Peptides
- Listeria monocytogenes in Food Safety
- Probiotics and Fermented Foods
- Food Chemistry and Fat Analysis
- Biotin and Related Studies
- Microbial Metabolic Engineering and Bioproduction
- Phytase and its Applications
- Biopolymer Synthesis and Applications
- Food composition and properties
- Animal Genetics and Reproduction
- Click Chemistry and Applications
- Monoclonal and Polyclonal Antibodies Research
- Biofuel production and bioconversion
- Essential Oils and Antimicrobial Activity
- Bacteriophages and microbial interactions
- Pineapple and bromelain studies
- Biochemical Analysis and Sensing Techniques
- Protein purification and stability
- Sensory Analysis and Statistical Methods
Utah State University
2015-2024
European Synchrotron Radiation Facility
2012
North Carolina State University
1993-2003
Biochemical Society
1999
University College London
1999
Harvard University Press
1987
Summary A whey‐based puffed snack food was produced on a twin‐screw extruder to determine the effect of varying starch type (normal corn and pregelatinised waxy), protein (regular instantised whey concentrate) concentration (16%, 32% 40%) physical chemical characteristics an extruded‐expanded food. Increasing normal cornstarch significantly reduced extrudate expansion ratio, air cell size, water solubility index increased density, breaking force. Protein affected absorption water‐soluble...
Collagen is used for a variety of biomedical and pharmaceutical uses, such as osteoarthritis-related pain management, hypertension, tissue engineering, human implants, generally derived from porcine or bovine. these animals has limitations due to the risk disease transmission religious constraints. Therefore, this study investigated extraction collagen catfish (Silurus triostegus) waste. Acid-solubilized pepsin-solubilized were extracted skin, fin, head, bone, muscle. SDS-PAGE patterns...
The antimicrobial activities of sucrose monolaurate and a novel ester, lactose (LML), were tested. Gram-positive bacteria more susceptible than Gram-negative to both esters. minimal bactericidal concentrations LML 5 9.5 mM for Listeria monocytogenes isolates 0.2 2 Mycobacterium isolates.
This study investigated the effects of high intensity ultrasound (temperature, amplitude, and time) on inactivation indigenous bacteria in pasteurized milk, Bacillus atrophaeus spores inoculated into sterile Saccharomyces cerevisiae orange juice using response surface methodology. The variables were sonication temperature (range from 0 to 84°C), amplitude 216 μ m), time 0.17 5 min) response, log microbe reduction. Data analyzed by statistical analysis system software three models developed,...
Cloning technologies, including embryo splitting and nuclear transfer, were introduced into dairy cattle breeding in the early 1980s. With recent worldwide attention on cloning of sheep ("Dolly") cows ("Gene"), potential food safety concerns for products derived from cloned animals needs to be addressed. There has been no study composition milk produced by cows. In this preliminary study, we evaluated 15 lactating non-embryonic cell six non-cloned over a single season. The came five unique...
Abstract Crystallization behavior of anhydrous milk fat (AMF) was studied with the addition 0.025 and 0.05 % lactose monolaurate (LML). The crystallization at low (Δ T = 3 °C) high supercooling 6 °C). Polarized light microscopy laser turbidimetry indicated a delay in on LML or Tween 20 to AMF. High intensity ultrasound (HIU) applied AMF samples lower ( c 31 °C; Δ HIU application + induced p < 0.05) changing induction time τ ) °C from 34.20 ± 1.67 min 47.07 1.27 (AMF LML) 23.23 3.26 25.00...
Lactose monolaurate (LML) was previously found to be an antimicrobial against Listeria monocytogenes in culture medium at concentrations between 3 and 5 mg ml(-1) . In this study, the microbial inhibitory activity of LML dairy products inoculated with a 5-strain cocktail clinical isolates L. investigated. Addition concentration resulted 4.4, 4.0 4.2 log reductions 0.5% fat, 1% fat 3.25% milks, respectively; 4.1, 3.5 nonfat, 1.5% yogurts, both nonfat 2% cottage cheese. The effect only...
Caffeic acid was modified via transglucosylation using sucrose and dextransucrase from Leuconostoc mesenteroides B-512FMCM. Following enzymatic modification, a caffeic glucoside isolated by butanol separation, silica gel chromatography, preparative HPLC. The synthesized had molecular mass-to-charge ratio of 365 m/z, its structure identified as acid-3-O-α-d-glucopyranoside. production this acid-3-O-α-d-glucopyranoside at concentration 153 mM optimized 325 acid, 355 sucrose, 650 mU mL-1 in the...
ABSTRACT: : We extruded a dry mix of 2 parts whey protein concentrate (80% protein) and 1 part cornstarch using water, 0.1 N HCl, or 0.2 M NaOH as the liquid, adding calcium (1.69% w/w) to with water liquid. The functionality acceptability textured (TWP) extrudates was determined. Ground beef patties containing up 40% (w/w) hydrated TWP made base (TWP ) were acceptable consumer panel all patties. Additionally, incurred less cook loss diameter reduction after cooking, but cohesive than
Sugar esters are biodegradable, nonionic surfactants which have microbial inhibitory properties. The influence of the fatty acid chain length on properties lactose was investigated in this study. Specifically, monooctanoate (LMO), monodecanoate (LMD), monolaurate (LML) and monomyristate (LMM) were synthesized dissolved both dimethyl sulfoxide (DMSO) ethanol. Minimum concentrations (MIC) minimum bactericidal (MBC) determined growth media. LML most effective ester, exhibiting MIC values <0.05...
Synergistic effect between high-intensity ultrasound (HIU), UV-A light, and natural antimicrobials on microbial inactivation in milk was studied. Milk with either ε-poly(lysine) or Nisin subjected to HIU (30 s) then light (15 min). Statistically significant treatments were stored at 4 25°C for 24 hr. Escherichia coli K12 reduced by ~2 logarithmic cycles ε-poly(lysine). During storage, there decline microorganisms 4°C no detectable load 25°C. E. ~1 Nisin, during the population decreased...
ABSTRACT Current methods for the detection of pathogens in food and water samples generally require a preenrichment step that allows selective enrichment test organism. The objective this research was to eliminate an allow bacteria directly 30 min. A high-flow-rate, fluidized bed capture concentrate large (bacteria spores) small (protein) molecules developed. This format, ImmunoFlow, is volume independent uses beads (greater than 3 mm diameter) when capturing prevent sample clogging testing...
ABSTRACT We developed a method for concentrating pathogens from samples without enrichment. Immobilized gangliosides concentrated bacteria detection with real-time PCR. A sensitivity of ∼4 CFU/ml (3 h) in competing microflora was achieved. Samples had 40,000 CFU/ml. The variance less than one cycle.