The minimum inhibitory concentrations (MICs) of 6 different antibiotics (chloramphenicol, clindamycin, erythromycin, streptomycin, tetracycline and vancomycin) were determined for 143 strains lactic acid bacteria bifidobacteria using the Etest. Different MICs found species strains. Based on distribution these MIC values, most either susceptible or intrinsically resistant to antibiotics. However, range some showed a bimodal distribution, which suggested that tested possess acquired antibiotic...

Abstract Currently, the food additive industry has interest in replacing artificial dyes with natural pigments, trying to maintain consumer and increase their awareness towards healthy diets. Phycobiliproteins are light-harvesting water-soluble proteins extracted from cyanobacteria red algae that can be employed as additives. In present work, a C-phycocyanin extract cyanobacterium Arthrospira platensis was tested blue colorant beverages demonstrate samples containing protein have color...

In the production of Spanish traditional blue-veined Cabrales cheese, lactic acid bacteria strains free antibiotic resistance that have a transferrable capacity are necessary as components specific starter. To select for these bacteria, minimum inhibitory concentration (MIC) 12 antibiotics and 2 mixtures (containing beta-lactamase inhibitor penicillin) were determined by microbroth agar dilution techniques in 146 belonging to genera Lactococcus, Enterococcus, Lactobacillus, Leuconostoc. The...

In spite of a global concern on the transfer antibiotic resistances (AR) via food chain, limited information exists this issue in species Leuconostoc and Weissella, adjunct cultures used as aroma producers fermented foods. work, minimum inhibitory concentration was determined for 16 antibiotics 34 strains dairy origin, belonging to mesenteroides (18), citreum (11), lactis (2), Weissella hellenica carnosum (1). Atypical were found kanamycin (17 strains), tetracycline chloramphenicol (two...

The knowledge regarding the intestinal microbial types involved in isoflavone bioavailabililty and metabolism is still limited. present work reports influence of a treatment with isoflavones for six months on faecal bacterial communities 16 menopausal women, as determined by culturing culture-independent techniques. Changes were analysed respect to women's equol-producing phenotype. Compared baseline, at 1 3 counts all populations faeces women had increased strongly. In contrast, among...

Twenty lactic acid bacteria (LAB) isolated from artisanal cheeses and previously selected for their technological properties were screened enzymatic activities, antimicrobial activity safety. The aim was to select those LAB strains that safe showed advantageous the development of starter cultures cheese making, discarding could transfer antibiotic resistance or produce any toxic biogenic amines. Aminopeptidase activities detected in most strains, particularly high substrate leucine...

Large antibiotic resistance gene pools in the microbiota of foods may ultimately pose a risk for human health. This study reports identification and quantification tetracycline- erythromycin-resistant populations, genes, diversity traditional Spanish Italian cheeses, via culturing, conventional PCR, real-time quantitative PCR (qPCR), denaturing gradient gel electrophoresis (DGGE). The numbers resistant bacteria varied widely among antibiotics different cheese varieties; some all bacterial...

The present work aimed to assess the susceptibility of dominant and representative bacterial populations from human gut isoflavones their metabolites. To do so, minimum inhibitory concentration (MIC) isoflavone glycosides, aglycones, equol 37 strains was determined by broth microdilution. Additionally, for 10 strains, growth curves, rate (μ), optical density (OD600 nm) cultures at 24 h were also determined. MICs daidzin, genistin, daidzein, genistein >2048 μg mL-1 all assayed, while that...

Lactic acid bacteria can act as reservoirs of antibiotic resistance genes that be ultimately transferred to pathogens. The present work reports on the minimum inhibitory concentration (MIC) 16 antibiotics 25 LAB isolates five Lactobacillus and one Bifidobacterium species from human vagina. Acquired resistances were detected kanamycin, streptomycin, chloramphenicol, gentamicin, ampicillin. A PCR analysis lactobacilli failed identify genetic determinants involved in any these resistances....

High throughput sequencing has recently revealed the presence of Tetragenococcus-related DNA sequences in dairy environments such as brine and cheeses. In present work, a selective medium was developed to isolate Tetragenococcus spp. from two ripened, traditional, Spanish, blue-veined cheese varieties made raw milk. The strains recovered belonged either koreensis or halophilus species. Twenty these isolates (15 T. 5 halophilus) were then subjected battery phenotypic genetic tests, six (4 2...

ABSTRACT All strains of Bifidobacterium animalis subsp. lactis described to date show medium level resistance tetracycline. Screening 26 from a variety sources revealed the presence tet (W) in all isolates. A transposase gene upstream was found strains, and both genes were cotranscribed strain IPLAIC4. Mutants with increased tetracycline as well tetracycline-sensitive mutants IPLAIC4 isolated genetically characterized. The native able restore phenotype mutant an alteration by functional...

This work introduces a novel real-time quantitative PCR (qPCR) protocol for detecting and quantifying equol-producing bacteria. To this end, two sets of primers targeting the dihydrodaidzein reductase (ddr) tetrahydrodaidzein (tdr) genes, which are involved in synthesis equol, were designed. The showed high specificity sensitivity when used to examine DNA from control bacteria, such as Slackia isoflavoniconvertens, equolifaciens, Asaccharobacter celatus, Adlercreutzia Enterorhabdus...

γ-Aminobutyric acid (GABA), an amino not used in protein synthesis, intervenes several physiological functions and has both diuretic calming effects humans. Lactic bacteria (LAB) strains that produce GABA could be exploited for the manufacture of health-promoting GABA-enriched dairy products. In this study, 262 LAB isolated from traditional products made raw milk without starter cultures were screened production culture media supplemented with 1% monosodium glutamate (MSG) using enzymatic...