A5083569423- Ovarian function and disorders
- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Estrogen and related hormone effects
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Hypothalamic control of reproductive hormones
- Birth, Development, and Health
- Growth Hormone and Insulin-like Growth Factors
- RNA and protein synthesis mechanisms
- Sexual Differentiation and Disorders
- Hormonal Regulation and Hypertension
- Reproductive Physiology in Livestock
- Reproductive System and Pregnancy
- Effects and risks of endocrine disrupting chemicals
- Virus-based gene therapy research
- Bacterial Genetics and Biotechnology
- Animal Genetics and Reproduction
- Hormonal and reproductive studies
- Inflammatory mediators and NSAID effects
- Stress Responses and Cortisol
- Antibiotic Resistance in Bacteria
- Neuroendocrine regulation and behavior
- T-cell and B-cell Immunology
- Cancer therapeutics and mechanisms
- Endometriosis Research and Treatment
St. Luke's Cystic Fibrosis Center of Idaho
2023
The Queen's Medical Research Institute
2008-2020
University of Edinburgh
2004-2020
MRC Centre for Reproductive Health
2006-2020
Queen's Medical Centre
2017-2019
The University of Melbourne
2018
Medical Research Council
2003-2016
GlaxoSmithKline (United Kingdom)
2001-2016
University of British Columbia
1979-2015
University of Tennessee at Knoxville
1976-2009
SUMMARY Two convenient bioassays of urinary gonadotrophins, using immature mice, are described. The first is based upon the initial doubling uterine weight. second, ovarian weight response, attempts to increase specificity follicle stimulating hormone by priming with human chorionic gonadotrophin. usefulness both methods discussed, and influence non-specific impurities during assay extracts stressed.
Abstract SB‐219383 and its analogues are a class of potent specific inhibitors bacterial tyrosyl‐tRNA synthetases. Crystal structures these have been solved in complex with the synthetase from Staphylococcus aureus , bacterium that is largely responsible for hospital‐acquired infections. The full‐length enzyme yielded crystals diffracted to 2.8 Å resolution, but truncated version allowed resolution be extended 2.2 Å. These not only occupy known substrate binding sites unique ways, also...
Implantation requires communication between a receptive endometrium and healthy blastocyst. This maternal-embryonic crosstalk involves local mediators within the uterine microenvironment. We demonstrate that secreted protein, prokineticin 1 (PROK1), is expressed in during early pregnancy. PROK1 induces expression of leukemia inhibitory factor (LIF) endometrial epithelial cells first trimester decidua via Gq-Ca2+-cSrc-mitogen-activated protein kinase kinase-mediated pathway. show human...
Spermatogenesis is a complex process reliant upon interactions between germ cells (GC) and supporting somatic cells. Testicular Sertoli (SC) support GCs during maturation through physical attachment, the provision of nutrients, protection from immunological attack. This role facilitated by an active cytoskeleton parallel microtubule arrays that permit transport nutrients to GCs, as well translocation spermatids seminiferous epithelium maturation. It established chemical perturbation SC...
Abstract C5‐normal B10.D2/new line mice were susceptible to passively transferred arthritis from purified type II collagen antibody, and in vitro studies demonstrated that, when bound cartilage, this antibody readily activated complement C5 C5a. C5‐deficient B10.D2/old failed develop arthritis, despite the deposition of C3 along cartilage surface. C57B1/6 which was characterized histopathologic as an erosive synovitis involving multiple inflammatory cell types. In contrast, neither clinical...
A three base-pair deletion in the widely expressed TOR1A gene causes childhood onset, neurological disease of DYT1 dystonia. Mouse Tor1a knockout also specifically affects developing nervous system. However, both cases, basis neuronal tissue specificity is unknown. TorsinA one four predicted mammalian torsin ATPases associated with assorted cellular activities (AAA+) proteins, raising possibility that expression a functionally homologous compensates for torsinA loss non-neuronal tissues. We...
Abstract Background Endometrial cancer is the most common gynaecological malignancy; risk factors include exposure to oestrogens and high body mass index. Expression of enzymes involved in biosynthesis prostaglandins (PG) often higher endometrial cancers when compared with levels detected normal endometrium. Oestrogens bind one two receptors (ERα ERβ) encoded by separate genes. The full-length function as ligand-activated transcription factors; splice variant isoforms ERβ lacking a...
Context:The human endometrium has an exceptional capacity for repeated repair after menses, but its regulation remains undefined. Premenstrually, progesterone levels fall and prostaglandin (PG) F2α synthesis increases, causing spiral arteriole constriction. We hypothesized that withdrawal, PGF2α, hypoxia increase vascular endothelial growth factor (VEGF), endometrial factor.
Does loss of DMRT1 in human fetal testis alter testicular development and result dysgenesis?DMRT1 repression alters the expression key ovarian determining genes, leads to focal dysgenesis.Testicular dysgenesis syndrome (TDS) is associated with common disorders young men, but its etiology unknown. has been shown play a role regulation sex differentiation vertebrate gonad. Downregulation male mice results trans-differentiation Sertoli cells into granulosa (FOXL2+) resulting an gonadal...
Dendritic cells (DC) are specialized sentinel that bridge the innate and adaptive immune response play a crucial role in shaping response. Vitamin D, known epidemiological risk factor for development of several autoimmune diseases, influences dendritic cells. Consequently, vitamin D metabolites frequently used protocols to develop therapeutic cell therapies diseases. However, mechanisms by which modulates DC function remain poorly understood. We investigated effects on murine CD11c+ bone...
Pro-inflammatory mediators, like prostaglandin (PG) and chemokines, promote tumourigenesis by enhancing cell proliferation, migration of immune cells recruitment blood vessels. Recently we showed elevated expression the chemokine (C-X-C motif) receptor 2 (CXCR2) in endometrial adenocarcinomas localized to neutrophils neoplastic epithelial vascular cells. Furthermore found that PGF(2alpha)-F-prostanoid (FP) regulates CXCR2 ligand CXCL1, neutrophil chemotaxis adenocarcinomas. In present study...
Prokineticin 1 (PROK1) signalling via prokineticin receptor (PROKR1) regulates the expression of several genes with important roles in endometrial receptivity and implantation. This study investigated PROK1 regulation Dickkopf (DKK1) expression, a negative regulator canonical Wnt signalling, its function non-pregnant endometrium first trimester decidua. DKK1 mRNA is elevated during mid-secretory phase menstrual cycle increases further protein localized to glandular epithelial stromal cells...
Abdominal surgery and disease cause persistent abdominal adhesions, pelvic pain, infertility occasionally, bowel obstruction. Current treatments are ineffective the aetiology is unclear, although excessive collagen deposition a consistent feature. Lysyl oxidase (Lox) key enzyme required for crosslinking of insoluble collagen, so we investigated whether targeting Lox might be an approach to reduce adhesions.Female C57Bl/6 mice were treated intraperitoneally with multiwalled carbon nanotubes...
SUMMARY Urinary gonadotrophins were assayed against HMG 20 A during the menstrual cycle in four subjects by methods believed to measure follicle stimulating hormone (FSH) and total gonadotrophin respectively. The showed qualitative fluctuation. Three maximum levels of near middle cycle, three a notably different pattern excretion FSH with high values first part cycle. Gonadotrophins from patients secondary amenorrhoea no fluctuation, but treatment stilboestrol apparently caused rise...
SUMMARY In a four‐centre prospective double‐blind trial, 108 patients with ulcerative colitis in remission were randomized to receive balsalazide doses of 3 g or 6 g/day for 12 months. The assessed at 3‐monthly intervals clinically, sigmoidoscopically and routine haematology biochemistry. Remission rates 77% (3 g/day) 68% (6 months not significantly different. Intolerance reactions leading withdrawal from the study occurred only 9 (8%), all occurring first 7 weeks study. Balsalazide is...
Abstract The asynchronous secretion of gonadotrope LH and FSH under the control GnRH is crucial for ovarian cyclicity but underlying mechanism not fully resolved. Because prostaglandins (PG) are autocrine regulators in many tissues, we determined whether they have this role gonadotropes. We first demonstrated that stimulates PG synthesis by induction cyclooxygenase-2, via protein kinase C/c-Src/phosphatidylinositol 3′-kinase/MAPK pathway LβT2 cell line. then PGF2α PGI2, PGE2 inhibited...
Prokineticins and their receptors are expressed in various cellular compartments human endometrium, with prokineticin 1 (PROK1) showing a dynamic pattern of expression across the menstrual cycle during pregnancy. Previous studies suggest that PROK1 can play an important role implantation early pregnancy by inducing vascular remodeling increasing permeability. Here we demonstrate induces IL-8, chemokine angiogenic properties, endometrial epithelial Ishikawa cells stably expressing receptor...