A5031313600- Plant Disease Resistance and Genetics
- Plant Pathogens and Resistance
- Genetics and Plant Breeding
- Plant Virus Research Studies
- Chromosomal and Genetic Variations
- Sugarcane Cultivation and Processing
- Plant Pathogenic Bacteria Studies
- Plant nutrient uptake and metabolism
- Plant Pathogens and Fungal Diseases
- Plant tissue culture and regeneration
- Plant Disease Management Techniques
- Genetic and Environmental Crop Studies
- Seed Germination and Physiology
- Photosynthetic Processes and Mechanisms
- Plant pathogens and resistance mechanisms
- Genetic Mapping and Diversity in Plants and Animals
- Banana Cultivation and Research
- Potato Plant Research
- Fern and Epiphyte Biology
- Spectroscopy and Chemometric Analyses
- Advances in Cucurbitaceae Research
- Plant Stress Responses and Tolerance
- Plant Taxonomy and Phylogenetics
- Plant Reproductive Biology
- Plant Genetic and Mutation Studies
Agricultural Research Service
2007-2024
Michigan State University
2012-2022
United States Department of Agriculture
2012-2022
University of Wisconsin–Madison
1994-2005
Plant (United States)
2001
University of Michigan
1991-1993
University of California, Davis
1990-1993
PCR allelic discrimination technologies have broad applications in the detection of single nucleotide polymorphisms (SNPs) genetics and genomics. The use fluorescence-tagged probes is leading method for targeted SNP detection, but assay costs error rates could be improved to increase genotyping efficiency. A new assay, rhAmp, based on RNase H2-dependent (rhPCR) combined with a universal reporter system attempts reduce from primer/primer primer/probe dimers while lowering compared existing...
A contiguous assembly of the inbred 'EL10' sugar beet (Beta vulgaris ssp. vulgaris) genome was constructed using PacBio long-read sequencing, BioNano optical mapping, Hi-C scaffolding, and Illumina short-read error correction. The EL10.1 540 Mb, which 96.2% contained in nine chromosome-sized pseudomolecules with lengths from 52 to 65 31 contigs a median size 282 kb that remained unassembled. Gene annotation incorporating RNA-seq data curated sequences via MAKER pipeline generated 24,255 gene...
Cultivated beets (Beta vulgaris ssp. vulgaris), including sugar beet, rank among the most important crops. The wild ancestor of beet crops is sea Beta maritima. Species and subspecies are readily crossable with cultivated thus available for crop improvement. To study genomic relationships in genus Beta, we sequence analyse 606 genomes, encompassing B. v. adanensis, macrocarpa, patula. We observe two genetically distinct groups beets, one from Atlantic coast other Mediterranean area. Genomic...
Ten genome-specific probes were developed from Brassica napus and B. oleracea genomic DNA libraries. Selection was based on polymorphism between limited variation within genomes, permitting their localization six individual C-genome chromosomes. Chromosome assignment accomplished by using two sets of campestris – alien addition lines derived (i) (ii) the artificially synthesized 'Hakuran'. The presence shared fragments A, B, C genomes indicates partial homology three genomes. However,...
Nucleotide-binding (NB-ARC), leucine-rich-repeat genes (NLRs) account for 60.8% of resistance (R) molecularly characterized from plants. NLRs exist as large gene families prone to tandem duplication and transposition, with high sequence diversity among crops their wild relatives. This can be a source new disease resistance, but difficulty in distinguishing specific sequences homologous family members hinders characterization improving crop varieties. Current genome sequencing assembly...
Diversification on the basis of utilization is a hallmark Beta vulgaris (beet), as well other crop species. Often, improvement and management activities are segregated by type, thus preserving unique genome diversity organization. Full interfertility typically retained in crosses between these groups more traits may be accessible if genetic type lineage were known, along with available markers to effect efficient transfer (e.g., via backcrossing). L. (2n =18) species complex composed...
In sugarbeet ( Beta vulgaris subsp. ), many linkage maps have been constructed, but the availability of markers continues to limit utility genetic in public domain programs. Here a framework map is presented that expandable and transferable research programs interested locating their on consensus map. its current framework, primary used were amplified fragment length polymorphisms (AFLPs) anchored Butterfass chromosome‐nomenclature groups using group specific validated other populations....
The chromosome complement of Brassica oleracea (2n = 18) was dissected by means alien addition lines generated successive backcrosses either two B. campestris accessions 20) to the resynthesized napus 'Hakuran' 38). Alien were characterized counts, morphology, pollen and seed fertility, transmission chromosome-specific markers. Mean number in first backcross generation approximately 23.5 little influenced accession. Fertility isozyme marker also not affected choice Transmission markers BC 2...
Restriction maps for rRNA genes of the three cultivated diploid species Brassica nigra, B. oleracea, and campestris are described using rDNA map radish as a standard. subunit heterogeneity similar to that found in was observed species, thus resulting complex hybridization patterns. could be distinguished from other two diploids by absence EcoRI site E3 subunits almost all accessions tested, its smaller external intergenic spacer. Two probes covering specific regions well heterologous...