A5034239620- Neuroscience and Neural Engineering
- Supramolecular Self-Assembly in Materials
- Conducting polymers and applications
- Linguistic Studies and Language Acquisition
- Nonlinear Optical Materials Studies
- Nanofabrication and Lithography Techniques
- Advanced biosensing and bioanalysis techniques
- Electrochemical sensors and biosensors
- Italian Fascism and Post-war Society
- Analytical Chemistry and Sensors
- 3D Printing in Biomedical Research
- Advanced Sensor and Energy Harvesting Materials
- Microfluidic and Capillary Electrophoresis Applications
- Molecular Communication and Nanonetworks
- RNA Interference and Gene Delivery
- Polydiacetylene-based materials and applications
- Neural dynamics and brain function
- Educational and Social Studies
- Pickering emulsions and particle stabilization
- Advanced Memory and Neural Computing
- Italian Literature and Culture
- Nanoparticle-Based Drug Delivery
- Advancements in Transdermal Drug Delivery
- Piezoelectric Actuators and Control
- Enzyme Structure and Function
Delft University of Technology
2015-2021
University of Foggia
2020
Film Independent
2019
Microsystems (United Kingdom)
2018
Technical University of Denmark
2010-2015
University of Canterbury
2013-2015
University of Auckland
2015
MacDiarmid Institute for Advanced Materials and Nanotechnology
2013-2015
Wellington Hospital
2014
Ørsted (Denmark)
2010-2013
A surface modification of interdigitated gold microelectrodes (IDEs) with a doped polypyrrole (PPy) film for detection dopamine released from populations differentiated PC12 cells is presented. thin PPy layer was potentiostatically electropolymerized an aqueous pyrrole solution onto electrode surfaces. The conducting polymer during electropolymerization by introducing counter-ions in the monomer solution. Several were tested and resulting modifications characterized electrochemically to find...
Protein nanofibrils offer advantages over other nanostructures due to the ease in their self-assembly and versatility of surface chemistry available. Yet, an efficient general methodology for post-assembly functionalization remains a significant challenge. We introduce generic approach, based on biotinylation thiolation, multi-functionalization protein self-assembled from whey proteins. Biochemical characterization shows effects onto nanofibrils' surface, giving insights into changes...
In this paper we present a series of experiments showing that vertical self-assembled diphenylalanine peptide nanowires (PNWs) are suitable candidate material for cellular biosensing. We grew HeLa and PC12 cells onto PNW modified gold surfaces observed no hindrance cell growth caused by the nanostructures; furthermore studied properties PNWs investigating their influence on electrochemical behavior electrodes. The were functionalized with polypyrrole (PPy) chemical polymerization, therefore...
In this paper we demonstrate the fabrication and electrochemical characterization of a microchip with 12 identical but individually addressable measuring sites, each consisting set interdigitated electrodes acting as working electrode well two circular functioning counter reference in close proximity. The are made gold on silicon oxide substrate passivated by nitride membrane. A method for avoiding creation high edges at (known lift-off ears) is presented. design highly symmetric to...
This Article presents the development of a combined cell culture–biosensing platform using vertically aligned self-assembled peptide nanofibers. Peptide nanofibers were patterned on microchip containing gold microelectrodes to provide cells with 3D environment enabling them grow and proliferate. Gold functionalized conductive polymers for electrochemical detection dopamine released from PC12 cells. The assured close proximity release site, active surface sensor, thereby rendering it possible...
Manufacturing high throughput in vitro models resembling the tissue microenvironment is highly demanded for studying bone regeneration. Tissues such as have complex multiscale architectures inside which cells reside. To this end, engineering a microfluidic platform incorporated with three-dimensional (3D) microscaffolds and submicron/nanoscale topographies can provide promising model 3D cell cultures. There are, however, certain challenges associated goal, need to decorate large surfaces...
An investigation of the biophysical stability and safety properties crystallin amyloid nanofibrils – a potential novel bionanomaterial.
Our ability to dictate the colloid geometry is intimately related self-assembly. The synthesis of anisotropic colloidal particles currently dominated by wet chemistry and lithographic techniques. chemical offers limited particle geometries at bulk quantities. Lithographic techniques, on other hand, provide precise control over shape, although lower yields. In this respect, two-photon polymerization (2PP)1 has attracted growing attention due its automatically fabricate complex micro/nano...
Conducting polymer 3D microelectrodes have been fabricated for possible future neurological applications. A combination of micro-fabrication techniques and chemical polymerization methods has used to create pillar electrodes in polyaniline polypyrrole. The thin films obtained showed uniformity good adhesion both horizontal vertical surfaces. Electrodes with metal/conducting materials characterized by cyclic voltammetry the presence conducting film shown increase electrochemical activity when...
In this paper we discuss the fabrication and characterization of three dimensional (3D) micro- nanoelectrodes with goal using them for extra- intracellular studies. Two different types electrodes will be described: high aspect ratio microelectrodes studying communication between cells ultimately brain slice recordings small highly localized measurements Electrical electrochemical these as well results PC12 cell differentiation on chip presented discussed.
We present here an electrochemical sensor microsystem for the monitoring of pH. The all-polymeric device is comprised a cyclic olefin copolymer substrate, 200 nm-thin patterned layer conductive polymer (PEDOT), and 70 nm electropolymerized pH sensitive (polyaniline). patterning fluidic (microfluidic channels) (wiring electrodes) functional elements was achieved with single soft PDMS mold via embossing step process. A post-processing treatment ethylene glycol assured enhancement electrodes,...
Micro-patterned diamond has been investigated for numerous applications, such as biomimetic surfaces, electrodes cell stimulation and energy storage, photonic structures, imprint lithography, others. Controlled patterning of substrates moulds typically requires lithography-based top-down processing, which is costly complex. In this work, we introduce an alternative, cleanroom-free approach consisting the bottom-up growth nanocrystalline (NCD) micropillar arrays by chemical vapour deposition...
Protein nanofibrils and nanotubes are now widely accepted as having potential for use in the field of bionanotechnology. For this to be a feasible alternative existing technologies, there is need commercially viable source. Previous work has identified amyloid fibrils formed from crude crystallin proteins such source, since these can produced large quantities at low cost. Applications include templates formation nanowires or biosensing scaffolds. There remains number practical...
Abstract Protein nanofibrils self‐assembled from crystallin proteins, a waste material the fishing industry, have been identified as suitable to be used bionanoscaffold. In this study, successful method for functionalization of protein by immobilizing several enzymes industrial relevance (glucose oxidase, β‐galactosidase, pectinase, α‐amylase, and laccase) through glutaraldehyde cross‐linking approach is reported. The extent evaluated using gel electrophoresis, transmission electron...
Today's mechatronics relies on conventional transducers, i.e. lumped sensors and actuators with rigid construction. Future consumer products, medical devices manufacturing processes require sensing actuation systems high count density of individual transducer units. Such can be addressed as distributed transducers. Building transducers is economically unaffordable, an alternative solution needed. In this work we propose study a methodology to build such sensor actuator from soft bending...
Detected kidney stone cases are increasing globally, yet knowledge on the conditions for formation is lacking. Experimental approaches mimicking micro-environmental present in vivo can help scientists untangle intertwined physiochemical and biological phenomena leading to formation. As crystal nucleation often initiates at liquid-solid interfaces, interface morphology plays a significant role rate of nucleation. Within nephron, functional unit kidney, four segments be distinguished that...