A5047210235- Cutaneous Melanoma Detection and Management
- Immunotherapy and Immune Responses
- Inflammatory Bowel Disease
- Helicobacter pylori-related gastroenterology studies
- Genetic factors in colorectal cancer
- Colorectal Cancer Surgical Treatments
- Peptidase Inhibition and Analysis
- Colorectal Cancer Screening and Detection
- Melanoma and MAPK Pathways
- Cell Adhesion Molecules Research
- Periodontal Regeneration and Treatments
- Autoimmune and Inflammatory Disorders
- Cancer Immunotherapy and Biomarkers
- Monoclonal and Polyclonal Antibodies Research
- AI in cancer detection
- Protease and Inhibitor Mechanisms
- Cutaneous lymphoproliferative disorders research
- HIV Research and Treatment
- Oral and Maxillofacial Pathology
- Immune Cell Function and Interaction
- Gastroesophageal reflux and treatments
- Microscopic Colitis
- CAR-T cell therapy research
- Image Processing Techniques and Applications
- Infectious Encephalopathies and Encephalitis
Spitalul Clinic Colentina
2011-2024
Carol Davila University of Medicine and Pharmacy
2016-2024
Institutul National Victor Babes
2024
Matrix Research (United States)
2017
Regression in melanoma is a frequent biological event of uncertain prognostic value as the lesion exhibits heterogeneous phenotypical features, both at morphological and immunohistochemical level. In present study, we examined expression tissue inhibitors metalloproteinases (TIMP1, TIMP2 TIMP3) with regression. We specifically levels these TIMPs regressed components (RC) non-regressed (NRC) tumor compared their those melanomas. found that TIMP1 was overexpressed NRC melanomas partial...
Given the current outbreak of coronavirus disease 2019 (COVID‑19) and development implementation mass vaccination, data are being obtained by analyzing vaccination campaigns. In present study, 69 healthcare workers who were exposed to patients with severe acute respiratory syndrome coronavirus‑2 monitored for specific immunoglobulin (Ig)G IgA levels at different time periods. Prior after first round 21 days (when second dose vaccine was administrated) 24 an mRNA‑based vaccine. The basal IgG...
Mycobacteria identification is crucial to diagnose tuberculosis. Since the bacillus very small, finding it in Ziehl-Neelsen (ZN)-stained slides a long task requiring significant pathologist's effort. We developed an automated (AI-based) method of mycobacteria. prepared training dataset over 260,000 positive and 700,000,000 negative patches annotated on scans 510 whole slide images (WSI) ZN-stained (110 400 negative). Several image augmentation techniques coupled with different custom...
The presence of lymphovascular invasion (LVI) in urothelial carcinoma (UC) is a poor prognostic finding. This difficult to identify on routine hematoxylin–eosin (H&E)-stained slides, but considering the costs and time required for examination, immunohistochemical stains endothelium are not recommended diagnostic protocol. We developed an AI-based automated method LVI identification H&E-stained slides. selected two separate groups UC patients with transurethral resection specimens....
Methotrexate (MTX) is a folic acid analog with anti‑proliferative (anti‑neoplastic, cytotoxic), immunosuppressive and anti‑inflammatory properties, which has been used in the treatment of various cutaneous disorders, such as psoriasis, keratoacanthoma, pityriasis rubra pilaris, atopic dermatitis, mycosis fungoides, bullous skin diseases, systemic sclerosis, morphea, lupus erythematosus, dermatomyositis crusted scabies. Inhibition cell proliferation explained through its role blocking...
Wound healing process comprises a complex network of cells and molecules that are regulated in order to pursue tissue regeneration. Our study focused on the capacity alveolar blood clots (ABCs), platelet-rich fibrin (PRF) plasma rich growth factors (PRGF) induce vitro fibroblasts proliferation migration as measure Using cellular impedance with xCELLigence technology we quantified L929 fibroblast standard cell line presence 4 different ABCs 3 PRFs harvested from healthy individuals during...
Introduction . The clinical use of bioactive materials for bone augmentation has remained a challenge because predictability and effectiveness concerns, as well increased costs. purpose this study was to analyse the ability integrate substitutes by evaluating immunohistochemical expression platelet endothelial cell adhesion molecules, vascular growth factor, collagen IV, laminin, osteonectin, in vicinity grafts, enabling tissue revascularization appearance lamellae. There is lack vivo...
Ulcerative colitis (UC) is a chronic, relapsing inflammatory bowel disease that slightly increases the risk of colorectal cancer in patients with long-standing extended disease. Overexpression p53 and p21 colonic epithelia usually detected UC when no dysplasia histologically seen it used by pathologists as discriminator between regenerative changes intraepithelial neoplasia, well tissue biomarker useful to predict evolution toward malignancy. We present one-year prospective observational...
In the present study, we employed ddPCR and IHC techniques to assess prevalence roles of RAS RAF mutations in a small batch melanoma (n = 22), benign moles 15), normal skin samples 15). Mutational screening revealed coexistence BRAF NRAS melanomas nevi occurrence G12/G13 variants healthy skin. All investigated had driver or genes elevated p16 protein expression, indicating cell cycle arrest despite an increased mutational burden. V600 were identified 54% melanomas, 50%. The associated with...
Dendritic cells (DCs) are antigen-presenting with an important role in the innate and adaptive immune system. In skin lesions, cutaneous DCs (Langerhans cells, dermal plasmacytoid DCs) involved activation inflammatory benign as well malignant lymphoid proliferations. Density distribution of infiltrate can be helpful to differentiate benign, reactive from nature population. We performed a retrospective study including 149 patients: 35 mycosis fungoides, spongiotic dermatitis, psoriasis, lupus...
There are limited data available on the regression of fibrosis in hepatitis C virus (HCV) patients who have achieved sustained virologic response (SVR) after interferon‑free treatments. Moreover, a perfect method for assessing liver and its dynamics has not been established yet. The main objective this study was to evaluate aspartate aminotransferase platelet ratio index (APRI) Fibrosis‑4 (FIB‑4) scores with HCV registered SVR. We performed ROC curve analysis diagnostic performance APRI...
It is suggested that different neuropeptides are actively involved in the pathogenesis of Helicobacter pylori (H. pylori)-induced gastritis acting as important effectors neuroimmune complex interactions, but available data limited and contradictory. The aim this study was to determine whether chronic infection generates changes substance P (SP) vasoactive intestinal peptide (VIP) gastric level evaluate dependence these potential effects on degree bacterial colonization or severity...
Wound healing is a complex biologic process evolving in three phases: inflammation, proliferation, and tissue remodeling controlled by numerous growth factors cytokines. Oral mucosa wounds heal with significantly less important scars macrophages mast cells more myofibroblasts than cutaneous counterparts. We analyzed 32 oral for TGFbeta1, TGFbeta2, TGFbeta3, TNFalpha, PDGF BB FGF1 expression mesenchymal cells, endothelial macrophages, multinucleated giant cells. identified differences the of...
Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is a key in several intracellular and intercellular signaling pathways, with multiple functional structural roles. CEACAM1 expression melanoma often described the invading part of tumor has been associated increased cells invasion migration. We studied regressing versus non-regressing thin melanomas, knowing that phenomenon regression represents valuable model for understanding immunity. In melanoma, through homophilic...
Bacillary angiomatosis represents a cutaneous and systemic infection caused by Bartonella species, typically described in the past HIV-positive patients or associated with immunodeficiencies. More recent case reports had brought into attention probability that this entity may manifest otherwise healthy individuals, triggered trauma skin burns. The physiopathology of neoproliferative process is based on production angiogenetic molecules, such as vascular endothelial growth factor (VEGF) IL-8....
Chronic inflammation induced by Helicobacter pylori ( H. ) infection plays a major role in development of gastric cancer. However, recent findings suggested that progression and neoplastic transformation are more complex than previously believed could involve different factors modulate microenvironment influence host-pathogen interaction. Among these factors, myenteric plexus its potential adaptive changes received little attention. This study is aimed at identifying the impact -associated...