A5027228737- Epigenetics and DNA Methylation
- Regulation of Appetite and Obesity
- Neuropeptides and Animal Physiology
- Histone Deacetylase Inhibitors Research
- Receptor Mechanisms and Signaling
- Genetics and Neurodevelopmental Disorders
- Hypothalamic control of reproductive hormones
- Neurogenesis and neuroplasticity mechanisms
- Prenatal Substance Exposure Effects
- Biochemical Analysis and Sensing Techniques
- Nerve injury and regeneration
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurobiology and Insect Physiology Research
- Traumatic Brain Injury Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Cannabis and Cannabinoid Research
- Mitochondrial Function and Pathology
- Neuroendocrine regulation and behavior
- Neuroscience and Neuropharmacology Research
- Neurotransmitter Receptor Influence on Behavior
- Tryptophan and brain disorders
- Adipose Tissue and Metabolism
- Reproductive biology and impacts on aquatic species
- Nuclear Receptors and Signaling
Savitribai Phule Pune University
2016-2024
University of Illinois Chicago
2009-2019
Jesse Brown VA Medical Center
2009-2019
Alcohol Research Group
2015
Pennington Biomedical Research Center
2007-2008
Rashtrasant Tukadoji Maharaj Nagpur University
2003-2008
Louisiana State University System
2007
Background: Rapid tolerance to the anxiolytic effects of ethanol appears be an important factor in development alcoholism. Here, we investigated involvement amygdaloid histone deacetylases (HDAC)‐induced epigenetic changes rapid (RET). Methods: RET rats was induced by 2 injections administered 24 hours apart. Both ethanol‐tolerant and control were treated with HDAC inhibitor, trichostatin A (TSA), anxiety‐like behaviors measured. activity, (H3 H4) acetylation, neuropeptide Y (NPY) expression...
The agouti viable yellow (Avy) spontaneous mutation generates an unusual mouse phenotype of agouti-colored coat and adult-onset obesity with metabolic syndrome. Persistent production signaling protein in Avy mice antagonizes melanocortin receptors the hypothalamus. To determine how this disruption neuroendocrine circuits affects leptin transport across blood-brain barrier (BBB), we measured influx B6 control after development obesity, hyperleptinemia, increased adiposity. After iv bolus...
Innate anxiety appears to be a robust factor in the promotion of alcohol intake, possibly due anxiolytic effects self-medication with alcohol. Brain-derived neurotrophic (BDNF) and its downstream target, activity-regulated cytoskeleton-associated (Arc) protein, play role regulation synaptic function structure. In order examine BDNF-Arc system associated dendritic spines ethanol, we investigated acute ethanol exposure on anxiety-like behaviors alcohol-preferring (P) -nonpreferring (NP) rats....
Development of anxiety-like behaviours during ethanol withdrawal has been correlated with increased histone deacetylase (HDAC) activity and decreased brain-derived neurotrophic factor (BDNF) activity-regulated cytoskeleton-associated protein (Arc) gene expression in the amygdala. Furthermore, HDAC-mediated modifications play a role synaptic plasticity. In this study we used HDAC inhibitor trichostatin A (TSA) to determine whether inhibition could prevent withdrawal-induced deficits dendritic...
Recent studies have demonstrated the involvement of epigenetic mechanisms in psychiatric disorders, including alcoholism. Here, we investigated effects histone deacetylase (HDAC) inhibitor, trichostatin A (TSA) on amygdaloid HDAC-induced deacetylation and neuropeptide Y (NPY) expression anxiety-like alcohol-drinking behaviours alcohol-preferring (P) –non-preferring (NP) rats. It was found that P rats displayed higher behaviours, nuclear, but not cytosolic, HDAC activity, which associated...
Alcohol exposure in adolescence is an important risk factor for the development of alcoholism adulthood. Epigenetic processes are implicated persistence adolescent alcohol exposure-related changes, specifically amygdala. We investigated role histone methylation mechanisms persistent effects intermittent ethanol (AIE) Adolescent rats were exposed to 2 g/kg (2 days on/off) or n-saline (AIS) during postnatal (PND) 28–41 and used behavioral epigenetic studies. found that AIE caused a...
The neuropeptide Y (NPY) system of the central nucleus amygdala (CeA) has been shown to be involved in anxiety and alcoholism. In this study, we investigated molecular mechanisms by which NPY CeA regulates alcohol drinking behaviors using alcohol-preferring (P) rats as an animal model.Alcohol-preferring were bilaterally cannulated targeting infused with artificial cerebrospinal fluid (aCSF) or NPY. Alcohol anxiety-like assessed 2-bottle free-choice paradigm light/dark box (LDB) exploration...
Epigenetic mechanisms appear to play an important role in neurodevelopment. We investigated the effects of acute ethanol exposure on anxiety measures and function histone deacetylases (HDAC) DNA methyltransferases (DNMT) amygdala bed nucleus stria terminalis (BNST) adolescent rats. One hour after exposure, rats were subjected measures. A subset was exposed two doses (24 h apart) (2 g/kg) measure rapid tolerance anxiolysis. The HDAC DNMT activities mRNA levels isoforms measured BNST. lower...
Adolescent intermittent ethanol exposure causes long-lasting alterations in brain epigenetic mechanisms. Melanocortin and neuropeptide Y signaling interact are affected by the brain. Here, persistent effects of adolescent on alpha-melanocyte stimulating hormone, melanocortin 4 receptor, expression their regulation histone acetylation mechanisms were investigated adulthood. Male rats exposed to (2 g/kg, i.p.) or volume-matched saline from postnatal days 28 41 allowed grow day 92. Anxiety-like...
<b>Objective:</b> Chronic exposure to atrazine and other pesticides is reported cause metabolic disorders, yet information on effects of expression genes relevant mitochondrial function largely missing. In the present study, therefore, we investigated a battery nuclear- mitochondrial-encoded involved in oxidative phosphorylation (OXPHOS) human liver (HepG2) rat muscle (L6) cell lines due short-term exposure.<br><b>Materials Methods:</b> We have determined EC<sub>50</sub> values for...
Microsampling techniques, particularly dried blood spots (DBS), offer a minimally invasive alternative to venipuncture for biomarker profiling in endurance exercise. This study leverages NULISAseq, an ultra sensitive proteomics platform, analyze inflammatory responses athletes participating half–marathon. A cohort of six individuals (three and three sedentary controls) underwent longitudinal collection at five timepoints: pre run (R–7, R–1), mid–run (R), post (R+1, R+7). DBS samples were...
Alcohol exposure affects neuronal plasticity in the adult and developing brain. Astrocytes play a major role modulating are target of ethanol. Tissue plasminogen activator (tPA) is involved by degrading extracellular matrix proteins including fibronectin laminin up-regulated ethanol vivo. In this study we explored hypothesis that DNA methylation astrocytes thereby increasing expression release tPA. It was found increased tPA mRNA levels, an effect mimicked inhibitor methyltransferase (DNMT)...
Abstract We studied the organization of neuropeptide Y (NPY)‐immunoreactive system in forebrain adult male cichlid fish Oreochromis mossambicus and its response to castration testosterone replacement by using morphometric methods. Immunoreactivity for NPY was widely distributed forebrain, pattern generally resembled that other teleosts. Whereas immunoreactivity conspicuous ganglia nervus terminalis (NT; or nucleus olfactoretinalis), a weak reaction detected some granule cells olfactory bulb...