A5072506396- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
- Plant Pathogens and Fungal Diseases
- Rice Cultivation and Yield Improvement
- Genetic Mapping and Diversity in Plants and Animals
- GABA and Rice Research
- Fungal and yeast genetics research
- Plant tissue culture and regeneration
- Wheat and Barley Genetics and Pathology
- Plant Gene Expression Analysis
- Plant Pathogenic Bacteria Studies
- Genetics and Plant Breeding
- CRISPR and Genetic Engineering
- Plant Genetic and Mutation Studies
- Plant Virus Research Studies
- Mycotoxins in Agriculture and Food
- Chromosomal and Genetic Variations
- Plant and Fungal Interactions Research
- Aluminum toxicity and tolerance in plants and animals
- Plant responses to water stress
- Microbial Natural Products and Biosynthesis
- Yeasts and Rust Fungi Studies
- Arsenic contamination and mitigation
- Silicon Effects in Agriculture
- Biofuel production and bioconversion
Central Rice Research Institute
2020-2025
Heinrich Heine University Düsseldorf
2024
National Research Centre on Plant Biotechnology
2013-2021
Indian Agricultural Research Institute
2012-2013
Rice (Oryza sativa) plays a significant role in achieving global food security. However, it suffers from several biotic and abiotic stresses that seriously affect its production. blast caused by hemibiotropic fungal pathogen Magnaporthe oryzae is one of the most widespread devastating diseases rice. The crop rice vulnerable to this seedlings adult plant stages affecting leaves, nodes, collar, panicles roots. This disease can be effectively managed through host resistance. Of 100 resistance...
Leaf rust is one of the most important diseases wheat and caused by Puccinia triticina, a highly variable pathogen prevalent worldwide. Decoding genome this will help in unraveling molecular basis its evolution identification genes responsible for various biological functions. We generated high quality draft sequences (approximately 100- 106 Mb) two races P. triticina; virulent Race77 old, avirulent Race106. The genomes 77 had 33X 27X coverage, respectively. predicted 27678 26384 genes, with...
Abstract Rhizoctonia solani , the causal agent of rice sheath blight disease, causes significant losses worldwide as there are no cultivars providing absolute resistance to this fungal pathogen. We have used Host Delivered RNA Interference (HD-RNAi) technology target two PATHOGENICITY MAP KINASE 1 ( PMK 1) homologues, RPMK 1-1 and 1-2, from R. using a hybrid RNAi construct. homologues in other pathogens essential for formation appressorium, infection structures required penetration plant...
Sheath blight disease (ShB), caused by the fungus Rhizoctonia solani Kühn, is one of most destructive diseases rice (Oryza sativa L.), causing substantial yield loss in rice. In present study, a novel chitinase gene, LOC_Os11g47510 was cloned from QTL region R. tolerant line Tetep and used for functional validation genetic transformation ShB susceptible japonica Taipei 309 (TP309). The transformants were characterized using molecular approaches. Molecular analysis PCR set primers specific to...
Abstract Stripe rust of wheat, caused by Puccinia striiformis f. sp. tritici , is one the important diseases wheat. We used NGS technologies to generate a draft genome sequence two highly virulent (46S 119 and 31) least (K) pathotypes P. from Indian subcontinent. generated ~24,000–32,000 contigs (N50;7.4–9.2 kb), which accounted for ~86X–105X depth coverage with an estimated size these ranging 66.2–70.2 Mb. A genome-wide analysis revealed that pathotype 46S might be evolved among three in...
Rice sheath blight disease caused by Rhizoctonia solani is one of the most devastating diseases in rice leading to heavy yield losses. Due polygenic nature resistance, no major resistance gene with complete host against R. has been reported. In this study, we have performed molecular and functional analysis genes associated solani-resistance QTL qSBR11-1 indica line Tetep. Sequence revealed presence a set 11 tandem repeats containing high degree homology class III chitinase defense response...
Abstract Rice blast resistance gene, Pi54 provides broad-spectrum against different strains of Magnaporthe oryzae . Understanding the cellular localization protein is an essential step towards deciphering its place interaction with cognate Avr -gene. In this study, we investigated sub-cellular Green Fluorescent Protein (GFP) as a molecular tag through transient and stable expression in onion epidermal cells ( Allium cepa ) susceptible japonica cultivar rice Taipei 309 (TP309), respectively....
Abstract Genetic diversity assessment and population structure analysis are essential for characterization of pathogens their isolates. Markers tools exploring genetic variation among the False smut rice caused by Ustilaginoidea virens , formerly Villosiclava is a major emerging disease in India. A high level variability observed at field level, but no information available from India on structure. This first report U . that included 63 isolates distributed across vast geographical area...
Rice is critical to global food security which demands immediate attention meet the ever-growing population. Development of improved variety major focus area research, in doubled haploid (DH) technology plays a vital role. Since, androgenesis shows its potential DH production, this method was not capitalized specially indica rice due recalcitrant nature tissue culture. Success governed by many important factors such as stage anther, pre-treatment conditions, accurate concentrations media,...
Cloning and functional characterization of plant pathogen inducible promoters is great significance for their use in the effective management diseases. The rice gene CYP76M7 was up regulated at 24, 48, 72 hours post inoculation (hpi) with two isolates Magnaporthe oryzae Mo-ei-11 Mo-ni-25. In this study, promoter cloned from cultivar HR-12, characterized functionally validated. Transcription Start Site mapped 45 bases upstream initiation codon. To validate promoter, 5' deletion analysis...
With the fast increase of human population and reduction in land areas under cultivation, it is essential to breed rice varieties with higher yield potential tolerance multiple stresses using modern breeding technologies. Till date, a large number hybrids have been developed India for different ecologies. However, average production 2600 kg/hectare. Since staple food grown ~43.86 million hectare, present productivity are not sufficient enough feed increasing population. Therefore, required...