A5046883036- Physiological and biochemical adaptations
- Aquaculture Nutrition and Growth
- Aquaculture disease management and microbiota
- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Meat and Animal Product Quality
- Growth Hormone and Insulin-like Growth Factors
- Animal Genetics and Reproduction
- Invertebrate Immune Response Mechanisms
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Marine Bivalve and Aquaculture Studies
- Autophagy in Disease and Therapy
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Heat shock proteins research
- Thyroid Disorders and Treatments
- Natural Antidiabetic Agents Studies
- Reproductive biology and impacts on aquatic species
- Genetics, Aging, and Longevity in Model Organisms
- Ocean Acidification Effects and Responses
- Natural Products and Biological Research
Universidad Andrés Bello
2016-2025
Interdisciplinary Center for Aquaculture Research
2016-2025
Universidad Michoacana de San Nicolás de Hidalgo
1992-2024
University of Concepción
2019-2024
University of Chile
2019
University of Valparaíso
2017
Hospital General De Segovia
2011
Millennium Institute for Integrative Biology
2006-2011
University of Liège
2000-2002
Austral University of Chile
1998
The insulin-like growth factor-I (IGF-I) is a key regulator of skeletal muscle in vertebrates, promoting mitogenic and anabolic effects through the activation MAPK/ERK PI3K/Akt signaling pathways. Nutrition also affects growth, activating intracellular pathways inducing protein synthesis accretion. Thus, both hormonal nutritional regulate mass. In this context, plasma IGF-I levels response to food were evaluated fine flounder using fasting refeeding trials. present study describes for first...
The IGF-binding proteins (IGFBPs) play a dual role in the regulation of activity and bioavailability IGFs different tissues. Diverse evidence has shown that IGFBPs can inhibit and/or potentiate IGF actions. In this study, igfbp1, 2, 3, 4, 5 , 6 were isolated fine flounder, flat fish species shows slow growth inherent Gh resistance muscle. Subsequently, expression all igfbp s was assessed skeletal muscle flounder underwent nutritional statuses. igfbp1 not expressed during any conditions,...
ABSTRACT Cortisol is an essential regulator of neuroendocrine stress responses in teleosts. predominantly affects target tissues through the genomic pathway, which involves interacting with cytoplasmic glucocorticoid receptors, and thereby, modulating stress‐response gene expressions. also produces rapid effects via non‐genomic pathways, do not involve transcription. Although cortisol‐mediated pathways are well documented teleosts, fully understood. Moreover, no studies have focused on...
Chronic stress detrimentally affects animal health and homeostasis, with somatic growth, thus skeletal muscle, being particularly affected. A detailed understanding of the underlying endocrine molecular mechanisms how chronic muscle growth remains lacking. To address this issue, present study assessed primary (plasma cortisol), secondary (key components GH/IGF system, muscular proteolytic pathways, apoptosis), tertiary (growth performance) responses in fine flounder ( Paralichthys adspersus)...
Environmental stressors, such as temperature, are relevant factors that could generate a negative effect on several tissues in fish. A key fish species for Chilean aquaculture diversification is the red cusk-eel (Genypterus chilensis), native which knowledge environmental stressors effects limited. This study evaluated of high-temperature stress liver control (14 °C) and (19 groups using multiple approaches: determination plasmatic hepatic enzymes (ALT, AST, AP), oxidative damage evaluation...
Ectothermic animals, such as teleosts, have increasingly been exposed to stressful high-temperature events due global warming. Currently, the effects of thermal stress on skeletal muscle, a key tissue for fish growth, are unknown. This study examined impact muscle transcriptome rainbow trout (Oncorhynchus mykiss) in control (15 °C) and (20 conditions. Additionally, we plasmatic levels cortisol, glucose, creatine kinase activity, oxidative damage autophagy activation muscle. High-temperature...
We isolated and characterized the tilapia (Oreochromis mossambicus) HSP70 gene, highly homologous to other genes. A dramatic increase of mRNA levels was observed after heat shock whole animals in all organs tested. Reporter constructs were tested for transient expression carp cells microinjected zebrafish embryos. The entire regulatory region (-851/+157) able mediate inducible reporter with no preference a particular tissue. Our studies represent first transcriptional analysis promoter from...
Cortisol is an essential regulator of neuroendocrine stress responses in teleost. performs its effects through the modulation glucocorticoid receptor (GR) and mineralocorticoid (MR), activating gene expression. Until now contribution both receptors global transcriptional response teleost skeletal muscle has not been explored. To understand a comprehensive manner how GR MR modulates transcriptomic response, we performed RNA-seq analysis. Juvenile rainbow trout ( Oncorhynchus mykiss )...
A detailed understanding of how the GH and IGF-I regulate muscle growth, especially in early vertebrates, is still lacking. The fine flounder a flatfish species exhibiting remarkably slow representing an intriguing model for elucidating growth regulatory mechanisms. Key components system were examined groups fish during periods feeding, fasting, refeeding. Under feeding conditions, there inherent systemic local (muscle) resistance, characterized by higher levels plasma than IGF-I, skeletal...
Skeletal muscle differentiation is a complex and highly regulated process characterized by cell cycle arrest, which associated with morphological changes including myoblast alignment, elongation, fusion into multinucleated myotubes. This balanced dynamically coordinated positive negative signals such as the insulin-like growth factor I (IGF-1) myostatin (MSTN), respectively. In this study, we report that stimulation of skeletal myoblasts during IGF-1 induces rapid transient calcium increase...
A description of the intracellular mechanisms that modulate skeletal muscle atrophy in early vertebrates is still lacking. In this context, we used fine flounder, a unique and intriguing fish model, which exhibits remarkably slow growth due to low production muscle-derived IGF-I, key factor has been widely acknowledged prevent revert atrophy. Key components system were examined species using detailed time-course sampling points, including two contrasting nutritional periods. Under basal...
Fish reared under intensive conditions are repeatedly exposed to stress, which negatively impacts growth. Although most fish follow a conserved pattern of stress response, with increased concentrations cortisol, each species presents specificities in the cell response and tolerance. Therefore, culturing new requires detailed knowledge these specific responses. The red cusk-eel (Genypterus chilensis) is economically important marine for Chilean aquaculture industry. However, there no...