A5021966593- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Seed Germination and Physiology
- Genomics and Phylogenetic Studies
- Ecology and Vegetation Dynamics Studies
- Viral gastroenteritis research and epidemiology
- Photosynthetic Processes and Mechanisms
- Insect symbiosis and bacterial influences
- Plant Molecular Biology Research
- Plant and animal studies
- Insect Pest Control Strategies
- Molecular Biology Techniques and Applications
- Respiratory viral infections research
- Fermentation and Sensory Analysis
- Child Nutrition and Water Access
- Health Systems, Economic Evaluations, Quality of Life
- Light effects on plants
- Infection Control in Healthcare
- Insect behavior and control techniques
- Microbial Community Ecology and Physiology
- Legume Nitrogen Fixing Symbiosis
- Bioinformatics and Genomic Networks
- Microbial Inactivation Methods
- Insect-Plant Interactions and Control
- Plant Reproductive Biology
University of York
2011-2025
ORCID
2015
University of Ottawa
1985-2005
Climate change is accelerating plant developmental transitions coordinated with the seasons in temperate environments. To understand importance of these timing advances for a stable life history strategy, we constructed full cycle model Arabidopsis thaliana. Modelling and field data reveal that cryptic function flowering time control to limit seed set winter annuals an ambient temperature window which coincides temperature-sensitive switch dormancy state. This coincidence predicted be...
Winter chilling is of central importance in the phenology temperate annual and perennial plants. Chilling accelerates flowering through process vernalization breaks both bud seed dormancy, permitting onset growth spring. The quantitative effects floral promotion winter Arabidopsis accessions are well-documented, but very little known about basic physiology underlying summer responses to chilling, which acts on seeds within soil bank. Here, we analyse response wild extended seeds, explore...
Despite their importance to aquatic ecosystems, global carbon cycling, and sustainable bioindustries, the genomes of photosynthetic bacteria contain large numbers uncharacterized genes. Here, we develop high-throughput endogenous fluorescent protein tagging in cyanobacterium Synechococcus elongatus PCC 7942. From 400 targets, successfully tag over 330 proteins corresponding >10% proteome. We use this collection determine subcellular localization, relative abundances, protein-protein...
The tsetse fly is the insect vector for Trypanosoma brucei parasite, causative agent of human African trypanosomiasis. colonization and spread trypanosome correlate positively with presence a secondary symbiotic bacterium, Sodalis glossinidius metabolic requirements interactions bacterium its host are poorly understood, herein we describe model S. metabolism. enabled design experimental verification defined medium that supports growth ex vivo This has been used subsequently to analyze in...
Abstract Background Industrial biotechnology will play an increasing role in creating a more sustainable global economy. For conventional aerobic bioprocesses supplying O 2 can account for 15% of total production costs. Microbubbles (MBs) are micron-sized bubbles that widely used industry and medical imaging. Using fluidic oscillator to generate energy-efficient MBs has the potential decrease costs associated with aeration. However, little is understood about effect on microbial physiology....
Robust systematic approaches for the metabolic engineering of cell factories remain elusive. The available models predicting phenotypical responses and mechanisms are incomplete, particularly within context compound toxicity that can be a significant impediment to achieving high yields target product. This study describes Multi-Omic Based Production Strain Improvement (MOBpsi) strategy is distinguished by integrated time-resolved systems analyses fed-batch fermentations. As case study,...
Streptomyces rimosus ATCC 10970 is the parental strain of industrial strains used for commercial production important antibiotic oxytetracycline. As an actinobacterium with a large linear chromosome containing numerous long repeat regions, high GC content, and single giant plasmid (GLP), these genomes are challenging to assemble. Here, we apply hybrid sequencing approach relying on combination short- long-read next-generation platforms whole-genome restriction analysis by using pulsed-field...
Productivity of bacterial cell factories is frequently compromised by stresses imposed recombinant protein synthesis and carbon-to-product conversion, but little known about these bioprocesses at a systems level. Production the unnatural metabolite citramalate in Escherichia coli requires expression single gene coding for synthase. Multiomic analyses fermentation producing 25 g liter-1 were undertaken to uncover reasons its productivity. Metabolite, transcript, protein, lipid profiles...
Lineage-specific expansion (LSE) of protein families is a widespread phenomenon in many eukaryotic genomes, but generally more limited bacterial genomes. Here, we report the presence 434 genes encoding solute-binding proteins (SBPs) from tripartite tricarboxylate transporter (TTT) family, within 8.2 Mb genome α-proteobacterium Rhodoplanes sp. Z2-YC6860, gene family over-representation unprecedented abundance prokaryotes. Representing over 6 % total number coding sequences, SBP are...
With continuing improvements and reducing costs of high-throughput technologies, microbiologists are increasingly collecting multi-omics datasets. However, the tools techniques used to analyse these kinds data often highly specialised require bioinformatics, statistics coding experience. Many studies also tend report on a single aspect whilst overlooking other potentially interesting phenomena. Consequently, many sets not being their full potential. MORF was created as solution problems by...
Abstract Despite their fundamental importance to aquatic ecosystems, global carbon cycling and exciting potential in sustainable bioindustries, the genomes of photosynthetic prokaryotes still contain large numbers uncharacterised protein-coding genes. Here we present a high-throughput approach for scarless endogenous fluorescent protein tagging model cyanobacterium Synechococcus elongatus PCC7942. From 400 targets successfully endogenously tag over 330 proteins corresponding >10%...
The development of new microbial growth and analytical techniques is becoming increasingly relevant in relation to ‘unculturable’ organisms. This may involve the modification existing methods or new, custom procedures. One important application this symbiotic bacteria insects. Symbionts, due adaptations their host, are often difficult culture vitro . With growing interest use modified microbiomes control vector-borne diseases, improved that further understanding an insect’s microbiome...