A5000109149- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Plant biochemistry and biosynthesis
- Ethics in Clinical Research
- Biomedical Ethics and Regulation
- Biochemical and biochemical processes
- Biochemical Acid Research Studies
- Health Systems, Economic Evaluations, Quality of Life
- Viral Infectious Diseases and Gene Expression in Insects
University of Nottingham
2015-2022
ABSTRACT Mevalonate diphosphate decarboxylase (MVD) is an ATP-dependent enzyme that catalyzes the phosphorylation/decarboxylation of ( R )-mevalonate-5-diphosphate to isopentenyl pyrophosphate in mevalonate (MVA) pathway. MVD a key engineered metabolic pathways for bioproduction isobutene, since it conversion 3-hydroxyisovalerate (3-HIV) important platform chemical. The putative homologue from Picrophilus torridus has been identified as highly efficient variant number patents, but its...
Bio-production of fuels and chemicals from lignocellulosic C5 sugars usually requires the use pentose phosphate pathway (PPP) to produce pyruvate. Unfortunately, oxidation pyruvate acetyl-coenzyme A results in loss 33 % carbon as CO2, detriment sustainability process economics. To improve atom efficiency, we engineered Escherichia coli utilize d-xylose constitutively using Weimberg pathway, allow direct production 2-oxoglutarate without CO2 loss. After confirming enzyme expression vitro, was...
Robust systematic approaches for the metabolic engineering of cell factories remain elusive. The available models predicting phenotypical responses and mechanisms are incomplete, particularly within context compound toxicity that can be a significant impediment to achieving high yields target product. This study describes Multi-Omic Based Production Strain Improvement (MOBpsi) strategy is distinguished by integrated time-resolved systems analyses fed-batch fermentations. As case study,...
Productivity of bacterial cell factories is frequently compromised by stresses imposed recombinant protein synthesis and carbon-to-product conversion, but little known about these bioprocesses at a systems level. Production the unnatural metabolite citramalate in Escherichia coli requires expression single gene coding for synthase. Multiomic analyses fermentation producing 25 g liter-1 were undertaken to uncover reasons its productivity. Metabolite, transcript, protein, lipid profiles...