A5050405476- Pluripotent Stem Cells Research
- RNA Research and Splicing
- 3D Printing in Biomedical Research
- RNA and protein synthesis mechanisms
- Mesenchymal stem cell research
- RNA Interference and Gene Delivery
- Surface Modification and Superhydrophobicity
- Cancer Cells and Metastasis
- Nuclear Structure and Function
- Cardiomyopathy and Myosin Studies
- Advanced Sensor and Energy Harvesting Materials
- Aerogels and thermal insulation
- Tissue Engineering and Regenerative Medicine
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neural Engineering
- Cellular Mechanics and Interactions
- RNA modifications and cancer
Agency for Science, Technology and Research
2014-2021
Gladstone Institutes
2021
National University of Singapore
2014-2018
Singapore Centre for Environmental Life Sciences Engineering
2014
Institute of Medical Biology
2014
Duke-NUS Medical School
2010
Mutations in the LaminA gene are a common cause of monogenic dilated cardiomyopathy. Here we show that mice with cardiomyocyte-specific Lmna deletion develop cardiac failure and die within 3-4 weeks after inducing mutation. When same mutations induced genetically deficient LINC complex protein SUN1, life is extended to more than one year. Disruption SUN1's function also accomplished by transducing expressing dominant-negative SUN1 miniprotein cardiomyocytes, using cardiotrophic Adeno...
Abstract Mutations in the cardiac splicing factor RBM20 lead to malignant dilated cardiomyopathy (DCM). To understand mechanism of RBM20-associated DCM, we engineered isogenic iPSCs with DCM-associated missense mutations as well knockout (KO) iPSCs. iPSC-derived heart tissues made from these cell lines recapitulate contractile dysfunction DCM and reveal greater than KO. Analysis RNA binding by eCLIP reveals a gain-of-function preference mutant for 3′ UTR sequences that are shared amyotrophic...
Epidermal stem cells have been in clinical application as a source of culture-generated grafts. Although applications for such are increasing due to aging populations and the greater incidence diabetes, current keratinocyte grafting technology is limited by immunological barriers time needed culture amplification. We studied feasibility using human fetal skin allogeneic transplantation showed that keratinocytes faster expansion times, longer telomeres, lower immunogenicity indicators,...
Cancer stem cells are speculated to have the capability of self-renewal and re-establishment tumor heterogeneity, possibly involved in potential relapse cancer. CD44+CD24−/lowESA+ been reported possess tumorigenic properties, these biomarkers thought be highly expressed breast cancer cells. Cell behavior can influenced by biomolecular topographical cues natural microenvironment. We hypothesized that different cell populations tissue exhibit adhesion characteristics on substrates with...
Abstract Parkinson’s Disease is a progressive neurodegenerative disorder attributed to death of mesencephalic dopaminergic (DA) neurons. Pluripotent stem cells have great potential in the study for this late-onset disease, but acquirement that are robust quantity and quality still technically demanding. Biophysical cues been shown direct cell fate, effect different topographies lineage commitment subsequent maturation stages less examined. Using human induced pluripotent (iPSCs), we applied...
Thermal jacket design using eco-friendly cellulose fibers from recycled paper waste is developed in this report. Neoprene as an outmost layer, aerogels the middle and Nylon innermost layer can form best sandwiched laminate zigzag stitching method for thermal development. The temperature of ice slurry inside water bottle covered with designed jackets remains at 0.1 °C even after 4 h, which average duration outfield exercise. Interestingly, insulation performance much better than commercial...
ABSTRACT RNA binding motif protein 20 (RBM20) is a key regulator of alternative splicing in the heart, and its mutation leads to malignant dilated cardiomyopathy (DCM). To understand mechanism RBM20-associated DCM, we engineered isogenic human induced pluripotent stem cells (iPSCs) with heterozygous or homozygous DCM-associated missense mutations RBM20 (R636S) as well knockout (KO) iPSCs. iPSC-derived heart tissues made from these cell lines recapitulated contractile dysfunction DCM revealed...