A5002822468- Radiopharmaceutical Chemistry and Applications
- Monoclonal and Polyclonal Antibodies Research
- Lymphoma Diagnosis and Treatment
- CAR-T cell therapy research
- Lung Cancer Research Studies
- Multiple Myeloma Research and Treatments
- Animal Behavior and Reproduction
- Immunotherapy and Immune Responses
- Click Chemistry and Applications
- Turtle Biology and Conservation
- Amphibian and Reptile Biology
- Fish Ecology and Management Studies
- Genetic diversity and population structure
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Peptidase Inhibition and Analysis
- Acute Lymphoblastic Leukemia research
- Cancer therapeutics and mechanisms
- Physiological and biochemical adaptations
- Viral Infectious Diseases and Gene Expression in Insects
- Synthesis and Biological Evaluation
- Retinoids in leukemia and cellular processes
- Neuroblastoma Research and Treatments
- Chronic Myeloid Leukemia Treatments
- Advanced Biosensing Techniques and Applications
- Plant and animal studies
Fred Hutch Cancer Center
2015-2023
Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa
2017-2023
Translational Therapeutics (United States)
2023
Cancer Research Center
2017
Seattle University
2010
Bates College
2003
University of California, Irvine
2002
University of Chicago
1998-1999
The University of Texas at Austin
1988
Predators are widely assumed to create selection that shapes the evolution of prey escape abilities. However, this assumption is difficult test directly due challenge recording both predation and its evolutionary consequences in wild. We examined these events by studying natural experimental populations Trinidadian guppies, Poecilia reticulata, which occur distinct high‐predation low‐predation environments within streams. Importantly, last two decades several guppies have been experimentally...
ABSTRACT Snapping turtles (Chelydra serpentina) demonstrate temperature-dependent sex determination (TSD): intermediate egg incubation temperatures (23–27 °C) produce males, while extreme females. are also sexually dimorphic: adult males typically larger than Previous researchers hypothesized that male-producing enhanced the growth rate of juvenile turtles, resulting in dimorphism and potentially providing an adaptive benefit for TSD. In reptiles, choice ambient temperature can influence...
Background Cell culture conditions during manufacturing can impact the clinical efficacy of chimeric antigen receptor (CAR) T cell products. Production methods have not been standardized because optimal approach remains unknown. Separate CD4 + and CD8 cultures offer a potential advantage but complicate may affect expansion function. In phase 1/2 trial, we observed poor separate hypothesized that coculture cells at defined ratio initiation would enhance simplify manufacturing. Methods We...
Temperature acclimation of adult vertebrates typically induces changes in metabolic physiology. During early development, such compensation might have profound consequences, yet metabolism is little studied life stages. We measured the effect egg incubation temperature on resting rate (RMR) and blood thyroid hormone levels hatchling snapping turtles (Chelydra serpentina). Like many reptiles, temperature‐dependent sex determination (TSD), which embryonic determines sex. Therefore, we designed...
In some reptiles, egg incubation temperature determines whether the embryo hatches as male or female; in others, sex chromosomes determine sex. A cloned gene (ZFY) representing putative testis-determining factor mammals was hybridized to genomic DNA of reptiles with and temperature-dependent determination. No differences hybridization patterns were observed. Hybridization ZFY polyadenylated RNA indicates that reptilian versions this are expressed embryos both sexes during...
Streptavidin (SA)-biotin pretargeted radioimmunotherapy (PRIT) that targets CD20 in non-Hodgkin lymphoma (NHL) exhibits remarkable efficacy model systems, but SA immunogenicity and interference by endogenous biotin may complicate clinical translation of this approach. In study, we engineered a bispecific fusion protein (FP) evades the limitations imposed system. Briefly, one arm FP was an anti-human antibody (2H7), with other anti-chelated radiometal trap for radiolabeled ligand...
Temperature acclimation may be a critical component of the locomotor physiology and ecology ectothermic animals, particularly those living in eurythermal environments. Several studies fish report striking biochemical kinetic properties isolated muscle. However, relatively few whole‐animal performance variable responses. We test hypothesis that different types locomotion will respond differently to temperature acclimation, probably due divergent physiological bases locomotion. studied two...
Chimeric antigen receptor (CAR)-based adoptive T-cell therapy is a highly promising treatment for lymphoid malignancies, and CD20 an ideal target antigen. We previously developed lentiviral construct encoding third generation CD20-targeted CAR but identified several features that required additional optimization before clinical translation. describe here improvements, including replacement of the immunogenic murine antigen-binding moiety with fully human domain, streamlining transgene insert...
Abstract Constitutive B-cell receptor signaling leads to overexpression of the antiapoptotic BCL-2 protein and is implicated in pathogenesis many types non-Hodgkin lymphoma (B-NHL). The small-molecule inhibitor venetoclax shows promising clinical response rates several lymphomas, but not curative as monotherapy. Radiotherapy a rational candidate for combining with inhibition, DNA damage caused by radiotherapy increases activity pro-apoptotic pathway proteins, lymphomas are exquisitely...
Predators are widely assumed to create selection that shapes the evolution of prey escape abilities. However, this assumption is difficult test directly due challenge recording both predation and its evolutionary consequences in wild. We examined these events by studying natural experimental populations Trinidadian guppies, Poecilia reticulata, which occur distinct high-predation low-predation environments within streams. Importantly, last two decades several guppies have been experimentally...
Pretargeted radioimmunotherapy (PRIT) has been investigated as a multi-step approach to decrease relapse and toxicity for high-risk acute myeloid leukemia (AML). Relevant factors including endogenous biotin immunogenicity, however, have limited the use of PRIT with an anti-CD45 antibody streptavidin conjugate radiolabeled DOTA-biotin. To overcome these limitations we designed anti-murine anti-human CD45 bispecific constructs using 30F11 BC8 antibodies, respectively, combined anti-yttrium...
Summary 1. The indicator theory of sexual selection suggests that mating displays honestly signal aspects fitness. While rarely studied, kinematic (locomotor) performance is an excellent candidate for honest indicator, as many animals include rapid or extended locomotion may be physiologically correlated with traits impact survival. 2. We investigate the value display wild‐caught male guppies, Poecilia reticulata , by examining relationships between traits, anti‐predator and survival during...
<div>Abstract<p>Streptavidin (SA)-biotin pretargeted radioimmunotherapy (PRIT) that targets CD20 in non-Hodgkin lymphoma (NHL) exhibits remarkable efficacy model systems, but SA immunogenicity and interference by endogenous biotin may complicate clinical translation of this approach. In study, we engineered a bispecific fusion protein (FP) evades the limitations imposed system. Briefly, one arm FP was an anti-human antibody (2H7), with other anti-chelated radiometal trap for...
<div>Abstract<p>Constitutive B-cell receptor signaling leads to overexpression of the antiapoptotic BCL-2 protein and is implicated in pathogenesis many types non-Hodgkin lymphoma (B-NHL). The small-molecule inhibitor venetoclax shows promising clinical response rates several lymphomas, but not curative as monotherapy. Radiotherapy a rational candidate for combining with inhibition, DNA damage caused by radiotherapy increases activity pro-apoptotic pathway proteins, lymphomas are...
<div>Abstract<p>Constitutive B-cell receptor signaling leads to overexpression of the antiapoptotic BCL-2 protein and is implicated in pathogenesis many types non-Hodgkin lymphoma (B-NHL). The small-molecule inhibitor venetoclax shows promising clinical response rates several lymphomas, but not curative as monotherapy. Radiotherapy a rational candidate for combining with inhibition, DNA damage caused by radiotherapy increases activity pro-apoptotic pathway proteins, lymphomas are...
<p>Table S1 presents mouse survival data for in vivo experiments</p>