Protein extracts obtained from cells or tissues often require removal of interfering substances for the preparation high-quality protein samples in proteomic analysis. A number extraction methods have been applied to various biological samples. TCA/acetone precipitation and phenol extraction, a common method is thought minimize degradation activity proteases as well reduce contaminants like salts polyphenols. However, relies on complete pulverization repeated rinsing tissue powder remove...

Drought is a major environmental threat that limits crop growth, development, and productivity worldwide. Improving drought resistance with genetic engineering methods necessary to tackle global climate change. It well known NAC (NAM, ATAF CUC) transcription factors play critical role in coping stress plants. In this study, we identified an factor ZmNAC20, which regulates response maize. ZmNAC20 expression was rapidly upregulated by abscisic acid (ABA). Under conditions, the...

Abstract Drought-induced osmotic stress severely affects the growth and yield of maize. However, mechanisms underlying different responses young old maize leaves to remain unclear. To gain a systematic understanding age-related responses, we compared osmotic-stress-induced changes in ages using multi-omics approaches. After short-term stress, suffered more severe water deficits than leaves. The adjustments transcriptomes, proteomes, hormones response were dynamic Metabolic activities,...

OPINION article Front. Plant Sci., 12 June 2018Sec. Proteomics and Protein Structural Biology Volume 9 - 2018 | https://doi.org/10.3389/fpls.2018.00802

Abstract Maize ( Zea mays L.) is one of the most important crops worldwide and a model organism among cereal crops. Abiotic biotic stresses are often present simultaneously severely influence maize production, causing great yield losses worldwide. Therefore, selection cultivation stress‐tolerant lines that adapt to various instrumental in addressing problem caused by stress. The mesocotyl crucial organ pushes shoots out deep water or soil after seed germination. It has simple anatomy...

1. Mycoplasma gallisepticum (MG) causes severe lung inflammation and cell damage by activating toll-like receptor (TLR) signalling, the nuclear factor-kappaB (NF-κB) pathway pro-inflammatory cytokine gene expression. Baicalin (BA) is a flavonoid extracted from Scutellaria baicalensis, which possesses anti-inflammatory anti-bacterial properties. This study investigated effect of BA in MG-induced its potential mechanism MG-infected chicken embryo lungs DF-1 cells.2. The histopathological...

Cysteine proteases (CPs) are vital proteolytic enzymes that play critical roles in various plant processes. However, the particular functions of CPs maize remain largely unknown. We recently identified a pollen-specific CP (named PCP), which highly accumulated on surface pollen. Here, we reported PCP played an important role pollen germination and drought response maize. Overexpression inhibited germination, while mutation promoted to some extent. Furthermore, observed germinal apertures...

Abstract Protein extracts obtained from cells or tissues often require removal of interfering substances for the preparation high-quality protein samples in proteomic analysis. A number extraction methods have been applied to various biological samples. TCA/acetone precipitation and phenol extraction, a common method is thought minimize degradation activity proteases as well reduce contaminants like salts polyphenols. However, relies on complete pulverization repeated rinsing tissue powder...

Currently, mechanical maize kernel harvesting has not been fully utilized in developing countries including China, partly due to the absence of suitable cultivars capable rapid desiccation during seed maturation. The initiation maturation is regulated by abscisic acid (ABA). For further characterization ABA-regulated key genes and cellular events, it necessary perform transcriptome analysis embryos. ABA synthesis-deficient mutant (vp5) normal (Vp5) seeds are materials for such purpose.In...

As a vital component of plant cell walls, proteins play important roles in stress response by modifying the structure walls and involving wall integrity signaling pathway. Recently, we have critically reviewed predictors, databases, cross-referencing subcellular locations possible (CWPs) plants (Briefings Bioinformatics 2018;19:1130–1140). Here, briefly introduce strategies for isolating CWPs during proteomic analysis. Taking maize (Zea mays) as an example, retrieved 1873 probable recorded...

Maize is one of the most widely cultivated crops. It accumulates a large quantity seed storage proteins, which are important for development and germination, contribute to nutritional quality seeds. Based on solubility, proteins divided into albumins (water-soluble), globulins (salt-soluble), prolamins (alcohol-soluble), glutelins (acid- or alkali-soluble). hybrids due superior performance F1 than that their parents, phenomenon known as heterosis. However, accumulation patterns in maize...

Maize starch is an important agricultural commodity that serves as food, feed, and a raw material for industrial purposes. Starch biosynthesis in maize endosperm essential process productivity under changing environmental conditions. However, the molecular mechanism underlying synthesis during development remains largely unknown. Recently, proteomic analyses of seeds have produced large amounts data, which significantly add to our understanding facilitate knowledge‐based improvements. In...

The mesocotyl connects the coleoptilar node and basal part of seminal root maize (Zea mays) seedling. pushes shoot seedling out soil during seed germination; thus, its growth is highly related to deep-sowing tolerance. Although many studies on have been carried at physiological molecular levels, proteomic changes associated with cellular activities are still unknown.In present study, hybrid Zhengdan 958 was used study accompanying protein changes. dark-grown etiolated mesocotyls exhibited a...

Abstract Starch is the major form of carbohydrate storage in plants and exists as discrete starch granules (SGs). Isolation high‐quality SGs different plant tissues a prerequisite for studying roles during growth, development, responses to abiotic stress. However, it difficult isolate transitory from leaves pollen grains due their small sizes low quantities. Herein, we develop novel method isolating by using aqueous two‐phase system (ATS) ethanol/NaH 2 PO 4 . The ATS efficiently separated...

Abstract Comparative proteomics is widely used to detect protein changes, especially differential abundance proteins (DAPs) that are involved in plant responses development, disease, or environment. Once DAPs identified, it essential validate any change their abundance, and role the biological process under study. In addition common confirmation by quantitative RT‐PCR, immunoblot, multiple reaction monitoring analysis, has been proposed enzyme activity assay (EAA) can be complementary...

ABSTRACT As the vital component of plant cell wall, proteins play important roles in stress response through modifying wall structure and involving integrity signaling. However, potential (CWPs) improvement crop tolerance has probably been underestimated. Recently, we have critically reviewed predictors, databases cross-referencing subcellular locations possible CWPs plants ( Briefings Bioinformatics 2018;19:1130-1140). In this study, taking maize Zea mays ) as an example, retrieved 1873...