A5059119798- HIV Research and Treatment
- CRISPR and Genetic Engineering
- Mosquito-borne diseases and control
- HIV/AIDS Research and Interventions
- Malaria Research and Control
- Reproductive tract infections research
- HIV/AIDS drug development and treatment
- interferon and immune responses
- Hepatitis C virus research
- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- Circular RNAs in diseases
- Genetics, Aging, and Longevity in Model Organisms
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- Hepatitis B Virus Studies
- Cancer-related molecular mechanisms research
- Biosensors and Analytical Detection
- Synthesis and Biological Evaluation
- Animal Disease Management and Epidemiology
- RNA regulation and disease
- HIV, Drug Use, Sexual Risk
- Renal Diseases and Glomerulopathies
- Uterine Myomas and Treatments
- MicroRNA in disease regulation
University of Cape Coast
2015-2023
Southern Medical University
2019-2022
University of Science and Technology of China
2017-2019
Center for Excellence in Molecular Cell Science
2017-2018
South China University of Technology
2013-2016
Bioscience (China)
2013-2015
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been continuously mutating since its first emergence in early 2020. These alterations have led this virus to gain significant difference infectivity, pathogenicity, and host immune evasion. We previously found that the open-reading frame 8 (ORF8) of SARS-CoV-2 can inhibit interferon production by decreasing nuclear translocation regulatory factor 3 (IRF3). Since several mutations ORF8 observed, therefore, present study, we...
HIV-1 CRF07_BC is one of the most common circulating recombinant forms (CRFs) in China. The question why and how expanded so rapidly remains unknown.
Asherman’s syndrome is an endometrial regeneration disorder resulting from injury to the basal layer, causing formation of scar tissue in uterus and cervix. This usually leads uterine infertility, menstrual disorders, placental abnormalities. While stem cell therapy has shown extensive progress repairing damaged endometrium preventing intrauterine adhesion, issues low engraftment rates, rapid senescence, risk tumorigenesis remain be resolved for efficient effective application this...
Long intergenic RNAs (lincRNAs) play critical roles in eukaryotic cells, but systematic analyses of the lincRNAs an animal for phenotypes are lacking. We generate CRISPR knockout strains Caenorhabditis elegans and evaluate their phenotypes. C. demonstrate global features such as shorter length fewer exons than mRNAs. For evaluation lincRNAs, we produce 155 total 170 lincRNAs. Mutants 23 show 6 analyzed traits. investigate these by phenotype gene expression patterns potential functional...
Most rapid diagnostic tests (RDTs) currently used for malaria diagnosis cannot distinguish the various Plasmodium infections. The development of a vivax specific RDTs with high sensitivity to sufficiently differentiate two most common infections would be very crucial disease treatment and control.Plasmodium aldolase gene (PvALDO) was amplified from extracted genomic DNA constructed into pET30a vector. protein successfully expressed in Escherichia coli soluble form overall purity over 95%...
Men who have sex with men (MSM) are vulnerable risk group for human immunodeficiency virus (HIV)-1 infection. However, some MSM do not disclose their same-sex behavior and could impact the transmission prevention of HIV-1 Here, we evaluated role nondisclosed in Guangzhou, China.The pol sequences were obtained from HIV-infected subjects 2008 to 2015. A network was constructed using HIV TRAnsmission Cluster Engine (HIV-TRACE) at a pairwise genetic distance 0.5%. The position determined by...
Misdiagnosis of malaria by commercial rapid diagnostic tests (RDTs) is a major cause concern in the diagnosis malaria. This retrospective study was aimed at assessing relative performance four RDTs with emphasis on detection two Plasmodium vivax antigens: aldolase and lactate dehydrogenase (LDH). Three commercially available LDH or antigen kits (One Step Malaria P.f/P.v, ParaHit Total ver. 1.0, SD Bioline Malaria) an anti-P. aldolase-specific monoclonal antibody (mAb) pair 1C3-12 F10 were...
Background Since human infection with the novel H7N9 avian influenza virus was identified in China March 2013, relatively high mortality rate and possibility of human-to-human transmission have highlighted urgent need for sensitive specific assays diagnosis infection. Methodology/Principal Findings We developed a rapid diagnostic test A (H7N9) using anti-hemagglutinin (HA) monoclonal antibodies specifically targeting H7 an immunochromatographic assay system. The limit detection 103.5 pfu/ml...
Purpose: Antibodies are key reagents in the development of immunoassay. We attempted to develop high-performance CPP immunoassays using high-affinity monoclonal antibodies prepared via cytokine-assisted immunization. Methods: used fetal liver tyrosine kinase 3 ligand (Flt3L), CC subtype chemokine 20 (CCL20), and granulocyte-macrophage colony-stimulating factor (GM-CSF) assist traditional subcutaneous immunization preparing antibodies, further immunoassay methods for CPP. Results: This novel...
Abstract Background Chlamydia trachomatis is the most common sexually transmitted infection and bacterial agent of trachoma globally. C. undergoes a biphasic developmental cycle involving an infectious elementary body replicative reticulate body. Little currently known about gene expression dynamics host cell mRNAs, lncRNAs, miRNAs at different stages development. Results Here, we performed RNA-seq miR-seq on HeLa cells infected with serovar E 20 h post-infection (hpi) 44 hpi or without...
Mutations in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have made this virus more infectious. Previous studies confirmed that non-structural protein 13 (NSP13) plays an important role immune evasion by physically interacting with TANK binding kinase 1 (TBK1) to inhibit IFNβ production. been reported NSP13; hence, the current study, biophysical and structural modeling methodologies were adapted dissect influence of major mutations NSP13, i.e., P77L, Q88H, D260Y, E341D,...
Background With the increasing effectiveness of antiretroviral therapy and shifting demographics, problem older people with HIV or AIDS is increasingly grim in China, neglecting infection among them may cause more serious social problems, exacerbate difficulty controlling transmission, increase risk death. Objective We investigated variations trends Chinese mortality by age, period, cohort, from 1990 to 2019, reveal relationship between burden, as well providing guidance for resource...
Human immunodeficiency virus type 1 (HIV-1) p24 protein is the most abundant viral of HIV-1. This secreted in blood serum at high levels during early stages HIV-1 infection, making it a biomarker for diagnosis. In this study, colloidal gold immunochromatographic assay (GICA) was established detecting using mouse monoclonal antibodies (mAbs). The expressed E. coli strain BL21 and purified used to immunize mice. Stable hybridoma cell lines secreting anti-p24 were obtained after ELISA screening...
A sensitive and specific monoclonal enzyme-linked immunosorbent assay (ELISA) for the determination of tissue-bound metabolite 3-amino-5-morpholinomethyl-2-oxazolidone (AMOZ) was developed. Three different haptens AMOZ were synthesized. The (I, III) derived from 4-carboxybenzaldehyde ethyl 4-bromobutyrate, respectively. Hapten II based on hapten I but a CN bond in parent structure reduced. Corresponding immunogens coating antigens prepared to improve sensitivity. hybridomas 4 × 108 secreting...
RNA viruses represent the most important pathogens for humans and animals exhibit rapid evolution high adaptive capacity, which is due to mutation rates using error-prone RNA-dependent polymerase (RdRp) during replication. The fidelity of RdRp closely associated with viral diversity, fitness, pathogenesis.