Spexin (SPX) and kisspeptin (KISS) are novel peptides relevant in the context of regulation metabolism, food intake, puberty reproduction. Here, we studied changes serum SPX KISS levels female non-obese volunteers (BMI<25 kg/m2) obese patients (BMI>35 kg/m2). Correlations between or with BMI, McAuley index, QUICKI, HOMA IR, insulin, glucagon, leptin, adiponectin, orexin-A, obestatin, ghrelin GLP-1 were assessed. Obese had lower as compared to (SPX: 4.48±0.19 ng/ml vs. 6.63±0.29 ng/ml;...

The purpose of the study was to examine effect 2 prebiotics and synbiotics on digestive potency pancreas in 1-, 3-, 7-, 14-, 21-, 34-day-old cockerels. Prebiotics (inulin Bi2 tos) + Lactococcus lactis subsp. tos cremoris) were injected ovo into air cell 12th d embryonic development. Their application increased activity amylase, lipase, trypsin pancreas. most pronounced changes observed at end investigated rearing period (d 34). strongest stimulative effects amylase shown by both synbiotics,...

A total of 1,200 Ross broiler chickens were used in 2 separate feeding studies to explore the effect myo-inositol (MYO) and phytase on performance blood biochemistry broilers fed diets formulated be either adequate or insufficient Ca digestible P (dP). Supplementation that dP with MYO resulted improved BW gain feed conversion ratio both experiments. However, these effects most pronounced finisher phase, moderate negative observed during starter period. diet microbial a similar extent as was...

Thirty-seven patients with methicillin-resistant Staphylococcus aureus infections and/or colonization were treated oral ciprofloxacin (750 mg twice a day). Clinical cure or improvement of occurred in 91% the patients, and bacteriologic 60%. Ciprofloxacin therapy suppressed S. 55% patients. Ciprofloxacin-resistant strains emerged 6 37

The effect of the isoflavone, genistein, on lipid metabolism ovariectomized rats was studied. Three types experiments were performed. In first one, fed diets supplemented with 0.01 or 0.1% genistein for 14 days. second and third experiments, direct liver fat tissue measured respectively by means perfusion incubation isolated adipocytes isoflavone. Genistein in food significantly decreased blood serum muscle triglyceride concentrations increased level free fatty acids serum. Serum cholesterol...

The effect of a combination carbohydrase and phytase enzymes on growth performance, insulin-like factor 1 gene expression, insulin status, receptor sensitivity in broiler chickens fed wheat-soybean meal diets containing 6% (starter) 12% (grower-finisher) full-fat rapeseed (canola type; low glucosinolate, erucic acid) from to 42 d age was studied. A total 510 one-day-old male were randomly assigned 3 dietary treatments, with 17 pens per treatment 10 birds pen. treatments consisted control...

Objectives Spexin is a peptide whose action poorly understood but which expressed in many tissues. This encouraged us to investigate the potential role of spexin regulation pancreatic secretion. Methods Cells/islets were incubated with different concentrations glucose and measure insulin MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays BrdU (5-bromo-2′-deoxyuridine) tests performed assess viability proliferation islets after treatment. Real-time polymerase chain...

Orexins are recently identified neuropeptides that appear to play a role in the regulation of energy homeostasis and arousal. They bind activate two closely related G protein-coupled receptors (OXR1 OXR2), previously described as orphans. In this study we examined involvement orexins insulin secretion from rat pancreatic islets utilizing an situ perfused pancreas isolated islet models. By means RT-PCR found both OXR1 OXR2 expressed islets. Furthermore, expression levels were higher than...

Leptin is an adipose tissue-secreted hormone, which decreases caloric intake and increases energy expenditure. Some effects of leptin on balance seem to be mediated by the hypothalamo-pituitary-thyroid axis. The present study was designed ascertain whether i) rat thyroid gland expresses long form receptor (ObRb) ii) prolonged administration (daily subcutaneous injections 24 nmol/kg for 6 consecutive days) affects thyroid-gland function in this species. Coupled RT-PCR, Western blotting...

Introduction The role of melatonin in human insulin regulation is poorly understood. Aim To investigate the influence supplementation on glucose and levels lipid metabolism blood serum liver. Methodology acute effects secretion male Wistar rats were investigated. In addition, carbohydrate was studied. vivo experiments, administered subcutaneously two different doses (0.5 1.0 mg/kg body weight, respectively), animals decapitated after 1 hour. Results higher dose hormone increased level blood....

In order to discover new strategies replace antibiotics in the post-antibiotic era meat-type chicken production, two synbiotics were tested: (Lactobacillus salivarius IBB3154 plus galactooligosaccharide (Syn1) and Lactobacillus plantarum IBB3036 raffinose family oligosaccharides (Syn2).The administered via syringe, using a special automatic system, into egg air chamber of Cobb 500 broiler chicks on 12th day incubation (2 mg prebiotics + 105 cfu bacteria per egg). Hatched roosters (total...

Abstract Background Obestatin has a role in regulating food intake and energy expenditure, but the roles of obestatin GPR39 receptor obesity type 1 2 diabetes mellitus (T1DM T2DM, respectively) are not well understood. The aim present study was to investigate changes pathophysiological conditions like obesity, T1DM, T2DM. Methods Using rat models diet‐induced (DIO), T1DM T2DM (n = 14 per group), obestatin, its precursor protein preproghrelin, expression investigated tissues involved glucose...

We compared the prevalence of Gram-negative bacilli in pharyngeal flora two groups patients with a known predilection for bacillary pneumonia (chronic alcoholics and diabetics), other aspiration-prone persons no (epileptics narcotic addicts), normal control subjects. Quantitative cultures saline gargles showed to be significantly (<i>P</i>&lt;.05) more prevalent among (35%) diabetics (36%) but not epileptics (17%) or addicts (20%) than controls (18%). Counts 100 per milliliter were also...

In the present study, influence of chromium(III) complexes (acetate, chloride, glycinate, histidinate, lactate and propionate) on insulin binding signal transduction [phosphorylation tyrosine serine in receptor substrate (IRS)-1] was investigated vitro using three experimental models: isolated rat liver membranes cultured mouse C2C12 myoblasts or 3T3-L1 preadipocytes. The examined did not elevate to membranes. Moreover, chromium lactate, acetate propionate diminished specific insulin....

Abstract The aim of the study was to investigate effect BCS (Body Condition Score) on calving day and its decrease during early lactation biochemical blood indices reproductive performance. One hundred thirty-one Polish Holstein-Friesian cows were divided into three groups according North-American body condition score (AC≤3.5 point BCS; MID 3.51-3.75 FAT&gt;3.75 BCS) in (HG &gt;0.49 WEL 0.49-0.25 BCS, L&lt;0.25 BCS). In current study, significant interaction between change time sampling BHBA...

Changes in thyroid status affect metabolism not only directly, but influence it also by alterations insulin secretion and action. Despite several investigations, these effects are, however, poorly characterised or even controversial. The aim of the studies was to investigate effect hyperthyreosis (HT) hypothyreosis (HPT) on binding rat liver membranes. Some metabolic parameters reflecting hormones action were determined. HT HPT developed daily administration for 3 weeks thyroxine (T (4) )...

The experiments were carried out on ovariectomized Wistar rats. Their livers perfused with basic perfusion medium (BPM) or BPM supplemented isoflavone genistein. insulin combination of the two factors. obtained results support hypothesis that genistein influences kinetics binding to cell membranes changing number receptors and dissociation constant (Kd) supplementation decreased high affinity (HAIR) both in treated untreated insulin. amount HAIR diminished significantly from 610±77 × 10-15...

The Deal-Grove model of thermal oxidation kinetics is adapted to cylindrically symmetric mesa structures and applied study steam AlAs. Oxidation process parameters are extracted from available experimental data as functions temperature the AlAs layer thickness. rate found be very sensitive not only temperature, but also front position inside mesa. slows down moves into mesa, reaches a minimum, then accelerates at final stages process. Complex nonmonotonic dependence on thickness revealed.

The pharmacokinetics of ciprofloxacin and its effect on the oropharyngeal bacterial flora were examined in 21 ill patients being treated for methicillin-resistant Staphylococcus aureus infections or colonization both. Oral (750 mg twice a day) produced peak trough concentrations serum 2.56 +/- 1.80 0.97 0.81 micrograms/ml steady-state salivary nasal secretory 1.29 1.0 1.84 0.91 micrograms/ml, respectively. Because technical problems, felt to represent rough approximations true values....