A5103076745- Celiac Disease Research and Management
- Microscopic Colitis
- Microbial Metabolites in Food Biotechnology
- Eosinophilic Esophagitis
- Helicobacter pylori-related gastroenterology studies
- Food Allergy and Anaphylaxis Research
- Wheat and Barley Genetics and Pathology
- Trypanosoma species research and implications
- Galectins and Cancer Biology
- Digestive system and related health
- Gastrointestinal disorders and treatments
- Monoclonal and Polyclonal Antibodies Research
- Microbial Metabolic Engineering and Bioproduction
- Viral Infections and Immunology Research
- Enzyme Production and Characterization
- E-Learning and Knowledge Management
- Enzyme Catalysis and Immobilization
- Transgenic Plants and Applications
- Diabetes and associated disorders
- Circadian rhythm and melatonin
- Voice and Speech Disorders
- Research on Leishmaniasis Studies
- Health and Medical Education
- Microbial Community Ecology and Physiology
- Microbial Inactivation Methods
Universidad de Sevilla
2014-2024
University of Carabobo
2017
Algarve Biomedical Center
2014
Rostov Research Institute of Microbiology and Parasitology
2012
Universidad de Oviedo
2004
Universidad del Salvador
2003-2004
Universidad de Navarra
2002
Centre Hospitalier Universitaire de Rennes
1997
Universidad de Extremadura
1996
Universidad Complutense de Madrid
1992-1995
This study evaluates polymorphonuclear neutrophil (PMN) cell performance in 61 diabetic patients free of infection (40 Type 1, 21 2), using tests that explore all the functional steps PMN: (1) adherence: expression adhesion molecules, CD 11a, 11b, 11c; nylon fiber adherence test; (2) chemotaxis under agarose towards bacterial oligopeptide FMLP and complement fractions, used as attracting agents; (3) phagocytosis opsonized latex microbeads; (4) bactericidal activity: chemiluminescence...
Gluten-free diet (GFD) is the only management for coeliac disease (CD). Available methods to assess GFD compliance are insufficiently sensitive detect occasional dietary transgressions that may cause gut mucosal damage. We aimed develop a method determine gluten intake and monitor in patients with CD evaluate its correlation damage.Urine samples of 76 healthy subjects 58 subjected different conditions were collected. A lateral flow test (LFT) highly specific G12 monoclonal antibody most...
Background/Aims: The aim of this exploratory trial was to establish if the probiotic Bifidobacterium natren life start (NLS) strain may affect clinical course and pathophysiological features patients with untreated celiac disease (CD). Positive findings would be helpful in directing future studies. Methods: Twenty-two adult having 2 positives CD-specific tests were enrolled. Patients randomized receive capsules before meals for 3 weeks either infantis super (Lifestart 2) (2×109...
Noninvasive serologic tests have shown high diagnostic accuracy for celiac disease (CD) in selected populations. Our aim was to determine prospectively the performance of CD-related serology individuals undergoing intestinal biopsy because clinical suspicion small-bowel disorders.We enrolled 141 unselected consecutive adult patients attending a clinic. Patients underwent endoscopy and biopsy; serum samples were obtained at that time measurements anti-tissue transglutaminase (a-tTG), IgA IgG...
In order to explore the diversity of extreme halophiles able produce different hydrolytic enzymes (amylase, protease, lipase and DNAse) in hypersaline habitats South Spain, a screening program was performed. A total 43 showing activities have been isolated characterized. The strains were grow optimally media with 15–20% (w/v) salts most cases, growth detected up 30% salts. Most hydrolase producers assigned family Halobacteriaceae, belonging genera Halorubrum (22 strains), Haloarcula (nine...
The efficacy of celiac disease (CD)-related antibodies in monitoring clinical outcome patients remains unclear. Our aims were to determine dynamics after diagnosis and assess their performances patients' long-term compliance with the gluten-free diet (GFD).We prospectively estimated performance seven disease-related antibody tests at 1 year more than 4 years treatment initiation 53 adults. ability identify partially compliant was explored by receiver operating characteristic curve analysis....
Abstract BACKGROUND: Cereals used for beer manufacturing contain gluten, which is immunotoxic celiac patients. The gluten remaining after processes of malting and brewing mostly hydrolyzed, makes practical evaluation the immunotoxicity pools challenging. RESULTS: We analyzed presence peptides equivalent to major protease‐resistant gliadin 33‐mer in 100 Belgium beers, using monoclonal antibodies (G12/A1). Immunochromatographic strips enzyme‐linked immonosorbent assay G12/A1 methods estimated...
We have previously shown a reduction of gastrointestinal symptoms after the oral administration Bifidobacterium infantis Natren Life Start super strain (NLS-SS) in untreated celiac disease (CD) patients. The symptomatic improvement was not associated with changes intestinal permeability or serum levels cytokines, chemokines, growth factors. Therefore, we hypothesized that beneficial effect observed patients CD treated B. may be related to modulation innate immunity.To investigate potential...
The screening and diagnosis of coeliac disease have been simplified by the advent new serological tools.To assess clinical utility a newly developed kit for antibodies to human recombinant tissue transglutaminase (hu-anti-tTG) in large population patients undergoing intestinal biopsy suspected disorders.We evaluated 426 serum samples from consecutive adult (250 untreated 176 individuals whom had excluded), obtained at time biopsy. Samples were tested immunoglobulin A (IgA) hu-anti-tTG...
To isolate and characterize the cultivable community of hydrolase producers (amylase, protease, lipase, DNase, xylanase pullulanase) inhabiting heavy-metal-contaminated soils in extreme conditions from Atacama Desert.A total 25 bacterial strains showing hydrolytic activities have been selected including halotolerants, extremely halotolerants moderate halophiles. Most were assigned to family B acillaceae, belonging genera Bacillus (nine strains), Halobacillus (seven strains) Thalassobacillus...
Background: Oats provide important nutritional and pharmacological properties, although their safety in coeliac patients remains controversial. Previous studies have confirmed that the reactivity of anti-33-mer monoclonal antibody with different oat varieties is proportional to immune responses terms T-cell proliferation. Although impact these on adaptive response has been studied, role dendritic cells (DC) still poorly understood. The aim this study characterize fractions effect DC from...
Gluten content from barley, rye, wheat and in certain oat varieties, must be avoid individuals with celiac disease. In most of the Western countries, level gluten food to considered as gluten-free products is below 20 parts per million measured by ELISA based on specific anti-gluten peptide antibody. However, beverages or suffering complex hydrolytic processes beers, relative proportion reactive peptides for patients analytical techniques may differ, because diversity resulting populations...
The only treatment available for coeliac disease (CD) is a strict diet in which the intake of wheat, barley, rye, or oats avoided. Barley major cereal crop, grown mainly its use brewing, and it has high nutritional value. identification varieties with reduced toxicity profile may contribute to improve diet, quality life health CD patients.Searching harmless barleys, we investigated accessions malting wild used developing new cultivated cereals. barleys was screened using G12 antibody cell...
Gluten is a complex of proteins present in barley, wheat, rye and several varieties oats that triggers celiac disease genetically predisposed subjects. notoriously difficult to digest by mammalian proteolytic enzymes therefore, proline-rich digestion-resistant peptides contain multiple immunogenic epitopes. Prolyl endopeptidases (PEP) hydrolyse internal proline residues on the carboxyl side have been proposed for food gluten detoxification as oral enzyme supplementation celiacs. The aim this...
Background Extensive use of phenolic compounds in industry has resulted the generation saline wastewaters that produce significant environmental contamination; however, little information is available on degradation conditions. Halomonas organivorans G-16.1 (CECT 5995T) a moderately halophilic bacterium we isolated previous work from environments South Spain by enrichment for growth different pollutants, including compounds. PCR amplification with degenerate primers revealed presence genes...