A5002916772- Genomics and Phylogenetic Studies
- Genetic diversity and population structure
- Photosynthetic Processes and Mechanisms
- Genetic Mapping and Diversity in Plants and Animals
- Plant Molecular Biology Research
- Gene expression and cancer classification
- Animal Behavior and Reproduction
- Plant nutrient uptake and metabolism
- Molecular Biology Techniques and Applications
- Plant Gene Expression Analysis
- Light effects on plants
- Chromosomal and Genetic Variations
- Neurobiology and Insect Physiology Research
- Genetic and phenotypic traits in livestock
- Wildlife Ecology and Conservation
- Primate Behavior and Ecology
- Plant Micronutrient Interactions and Effects
- Plant Stress Responses and Tolerance
- Legume Nitrogen Fixing Symbiosis
- Genomics, phytochemicals, and oxidative stress
- Insect-Plant Interactions and Control
- Identification and Quantification in Food
- Botany and Geology in Latin America and Caribbean
- Bioinformatics and Genomic Networks
- Fish Ecology and Management Studies
Ryukoku University
2016-2023
Kyushu Institute of Technology
2014-2018
Tohoku University
2011-2016
Hokkaido University
2013-2014
Well prepared It is well known that species radiate into new niches by adapting to novel environments. But why do some in this way, while other, related, not. Ishikawa et al. looked across sticklebacks determine some, originally marine, lineages were able colonize postglacial freshwater environments (see the Perspective Weber and Tong). They found a gene involved fatty acid desaturation was duplicated lineages. Transgenic manipulation of allowed marine synthesize acids thus survive on...
RNA-Seq is a whole-transcriptome analysis method used to research biological mechanisms and functions but its use in large-scale experiments limited by high cost labour requirements. In this study, we have established high-throughput cost-effective library preparation that does not require mRNA enrichment. The adds unique index sequences samples during reverse transcription (RT) conducted at higher temperature (≥62 °C) suppress RT of A-rich rRNA, then pools all into single tube. Both...
Summary Increase in the nitrogen (N)‐use efficiency and optimization of N response crop species are urgently needed. Although transcription factor‐based genetic engineering is a promising approach for achieving these goals, factors that play key roles to deficiency have not been studied extensively. Here, we performed RNA‐seq analysis root samples 20 Asian rice ( Oryza sativa ) accessions with differential nutrient uptake. Data obtained from plants exposed N‐replete N‐deficient conditions...
Leaves within crop canopies experience variable light over the course of a day, which greatly affects photosynthesis and productivity. Little is known about mechanisms photosynthetic response to fluctuating their genetic control. Here, we examined gas exchange, metabolite levels, chlorophyll fluorescence during induction in an Oryza sativa indica cultivar with high yield (Takanari) japonica lower (Koshihikari). Takanari had faster sudden increases intensity than Koshihikari, as demonstrated...
The differences between plants grown in field and controlled environments have long been recognized. However, few studies addressed the underlying molecular mechanisms. To evaluate plant responses to fluctuating using laboratory equipment, we developed SmartGC, a high-performance growth chamber that reproduces irradiance, temperature humidity of environments. We analysed massive transcriptome data rice under SmartGC conditions clarify Rice dynamics mimicked those field, particularly during...
Transgressive segregation produces hybrid progeny phenotypes that exceed the parental phenotypes. Unlike heterosis, extreme caused by transgressive are heritably stable. We examined of flowering time in rice, and revealed F2 populations derived from a cross between parents with similar (proximal) days to heading (DTH). The DTH A58 × Kitaake progenies were frequently more than those either parent. These maintained F3 F4 populations. Both japonica rice cultivars adapted Hokkaido, Japan, which...
The guppy (Poecilia reticulata) shows remarkable variation of photoreceptor cells in the retina, especially those sensitive to middle-to-long wavelengths light. Microspectrophotometry (MSP) has revealed varying "green", "green-yellow" and "yellow" cone among guppies Trinidad Venezuela (Cumana). In genome, there are four "long-wave" opsin loci (LWS-1, -2, -3 -4). Two LWS-1 alleles have potentially differing spectral sensitivity (LWS-1/180Ser LWS-1/180Ala). addition, two "middle-wave" (RH2-1...
When used in closed-type plant factories, light-emitting diode (LED) illumination systems have the particular advantages of low heat emission and high luminous efficiency. The effects quality intensity on growth morphogenesis many species been examined, but improvements are needed to optimize for better products with lower resource investments. In particular, new strategies reduce wastage related leaf senescence, control ingredients appearance leafy vegetables. Although light is often...
Phenotypic plasticity can have strong impacts on predator-prey interactions. Although much work has examined the effects of inducible defences, less understood is how offences in predators affect interactions and predator prey phenotypes. Here, we examine an offence life histories a cohort predatory Hynobius retardatus salamander larvae their prey, Rana pirica tadpoles. We larval (duration, survival) post-metamorphic (size) traits both species after manipulating presence/absence tadpoles...
The guppy (Poecilia reticulata) is an important model organism for studying sexual selection; male guppies have complex and conspicuous pigmentation, female exhibit preferences males with specific color spots. Understanding the genetic basis underlying pigmentation variation in exploring factors causing maintenance of polymorphism wild populations. We focused on melanic black guppies, examined variations melanocortin 1 receptor (MC1R) gene because this known to contribute melanin several...
Summary In most animal species, the hatchling stage is a highly vulnerable life‐history stage. many fish and amphibian abundance varies substantially among sites years, with result that selection strength in conspecific interactions such as cannibalism also variable. The variability of leads species to evolve phenotypic plasticity, adaptive trait changes hatchlings depend on density conspecifics can therefore be expected. However, plasticity strategies response cannibalistic this have...
Grassland ecosystems worldwide have been extensively converted to other land uses and are globally imperiled. Because many grasslands maintained by human activities, understanding their origin history is fundamentally important better contemporary management. However, existing methods reconstruct past vegetation can produce contrasting views on grassland history. Here, we inferred demographic histories of 40 populations four forb species throughout Japan using high-resolution genome...
How genetic variations affect gene expression dynamics of field-grown plants remains unclear. Expression quantitative trait loci (eQTL) analysis is frequently used to find genomic regions underlying polymorphisms. This approach requires transcriptome data for the complete set QTL mapping population under given conditions. Therefore, only a limited range environmental conditions covered by conventional eQTL analysis. We sampled sparse time series rice from chromosome segment substitution...
Recently, increasing attempts have been made to understand how plant genes function in natura. In this context, transcriptional profiles represent physiological status response environmental stimuli. Herein, we combined high-throughput RNA-Seq with insect survey data on 19 accessions of Arabidopsis thaliana grown at a field site Switzerland. We found that the gene ontology (GO) annotations "glucosinolate biosynthetic process" and "response insects" were most significantly enriched,...
Hybridization between divergent lineages generates new allelic combinations. One mechanism that can hinder the formation of hybrid populations is mitonuclear incompatibility, is, dysfunctional interactions proteins encoded in nuclear and mitochondrial genomes (mitogenomes) diverged lineages. Theoretically, selective pressure due to incompatibility affect genotypes a population which mitogenomes from admix. To directly thoroughly observe this key process, we de novo sequenced 747-Mb genome...
Lineage-specific duplicated genes likely contribute to the phenotypic divergence in closely related species. However, neither frequency of duplication events nor degree selection pressures immediately after gene is clear speciation process. Here, using Illumina DNA-sequencing reads from Arabidopsis halleri, which has multiple species with high-quality genome assemblies (A. thaliana and A. lyrata), we succeeded generating orthologous groups Brassicaceae. The retained lineage was ∼10 times...
Nuclear sequence markers are useful tool for the study of history populations and adaptation. However, it is not easy to obtain multiple nuclear primers organisms with poor or no genomic information. Here we used genomes that have been fully sequenced design comprehensive sets amplify polymorphic fragments genes in non-sequenced organisms. First, identified a large number candidate regions were flanked on each side by conserved reference genomes. We then designed based these sequences...
Phosphorus (P) is an essential macronutrient for plant growth. In deciduous trees, P remobilized from senescing leaves and stored in perennial tissues during winter further Annual internal recycling accumulation of are considered important strategy to support the vigorous growth trees. However, pathways seasonal re-translocation molecular mechanisms this transport have not been clarified. Here we show route visualized using real-time radioisotope imaging macro- micro-autoradiography. We...
Gene expression levels exhibit stochastic variations among genetically identical organisms under the same environmental conditions. In many recent transcriptome analyses based on RNA sequencing (RNA-seq), in gene replicates were assumed to follow a negative binomial distribution, although physiological basis of this assumption remains unclear. study, RNA-seq data obtained from Arabidopsis thaliana eight conditions (21-27 replicates), and characteristics gene-dependent empirical probability...
Abstract Latitude is correlated with environmental components that determine the distribution of biodiversity. In combination geographic factors, latitude‐associated variables are expected to influence speciation, but empirical evidence on how those factors interplay scarce. We evaluated genetic and variation among populations in pair sister species Dioon sonorense–D. vovidesii , two cycads distributed along a latitudinal gradient northwestern Mexico, reveal their demographic histories...
Taishu horses are a native Japanese breed, of which only 41 individuals remained on Tsushima Island in 2018. Their genetic diversity is considered lower than that other horse breeds; thus, it needs to be investigated for sustainable conservation this breed. Historical records revealed several were released areas off-Tsushima mid-1980s. At present, living outside Island, hereafter referred as Non-Tsushima Taishus (NTTs), tagged. However, the structure NTT remains unclear, and such not...