- Radioactive contamination and transfer
- Plant nutrient uptake and metabolism
- Radioactivity and Radon Measurements
- Plant Stress Responses and Tolerance
- Plant Micronutrient Interactions and Effects
- Radioactive element chemistry and processing
- Nuclear and radioactivity studies
- Rice Cultivation and Yield Improvement
- Plant responses to water stress
- Biopolymer Synthesis and Applications
- Bamboo properties and applications
- Salmonella and Campylobacter epidemiology
- Isotope Analysis in Ecology
- Aluminum toxicity and tolerance in plants and animals
- Plant Molecular Biology Research
- Photonic and Optical Devices
- Silicon Effects in Agriculture
- Radiation Detection and Scintillator Technologies
- Educational Robotics and Engineering
- Heavy Metals in Plants
- Nitrogen and Sulfur Effects on Brassica
- Seaweed-derived Bioactive Compounds
- Advanced Optical Sensing Technologies
- Lipid Membrane Structure and Behavior
- Photosynthetic Processes and Mechanisms
The University of Tokyo
2015-2024
Hoshi University
2020-2024
National Institute for Japanese Language and Linguistics
2023
National Agriculture and Food Research Organization
2023
RIKEN
2023
University of Tsukuba
2023
Ebara (Japan)
2020
Tokyo Institute of Technology
2012
Hokkaido University
1999-2009
Central Food Technological Research Institute
2008
Fucoxanthin (Fx) and fucosterol (Fs) are characteristic lipid components of brown seaweeds that afford several health benefits to humans. This article describes the quantitative evaluation lipids 15 species with specific reference Fx, Fs, functional long-chain omega-6/omega-3 polyunsaturated fatty acids (PUFAs). In addition, fatty-acid composition selected was also accomplished in study. Major omega-3 PUFAs analyzed were α-linolenic acid (18:3n-3), octadecatetraenoic (18:4n-3), arachidonic...
Abstract Cesium adsorption/desorption experiments for various clay minerals, considering actual contamination conditions in Fukushima, were conducted using the 137 Cs radioisotope and an autoradiography imaging plates (IPs). A 50 μl solution containing 0.185 ~ 1.85 Bq of (10 −11 10 −9 molL −1 Cs) was dropped onto a substrate where mineral particles arranged. It found that partially-vermiculitized biotite, which is termed “weathered biotite” (WB) this study, from Fukushima sorbed far more...
Participation of the intervascular transport system within rice stem during cadmium (Cd) partitioning was investigated by characterizing 109Cd behaviour in shoot. In addition, 45Ca, 32P, and 35S patterns were analysed for comparison with that 109Cd. Each tracer applied to seedling roots 15min, shoots harvested either at 15min (i.e. immediately after application) or 48h. Distribution each element studied identify primary pathway before remobilization initiated. 32P preferentially transported...
An adjustment-free method to eliminate the noise induced by interference between signal wave and backscattering in an optical passive ring-resonator gyro is proposed demonstrated. In this method, lightwave incident into ring resonator modulated binary shift keying its carrier component. This can easily be performed using acoustooptic modulator a double balanced mixer. experiment, successfully reduced without any adjustment of modulation parameters.< <ETX...
Ionic nutrition is essential for plant development. Many techniques have been developed to image and (or) measure ionic movement in plants. Nevertheless, most of them are destructive limit the analysis. Here, we present development radioisotope imaging that overcome such restrictions allow real-time movement. The first system, called macroimaging, was visualize ion uptake translocation between organs at a whole-plant scale. Such device fully compatible with illumination sample. We also...
Minerals and photosynthates are essential for many plant processes, but their imaging in live plants is difficult. We have developed a method Arabidopsis using real-time radioisotope system. When each radioisotope, 22 Na, 28 Mg, 32 P-phosphate, 35 S-sulfate, 42 K, 45 Ca, 54 Mn 137 Cs, was employed as an ion tracer, movement from root to shoot over 24 h clearly observed. The movements of P, S Cs were fast so that they spread the tip stems. In contrast, high accumulation Ca found basal part...
In plant research, radioisotope imaging provides useful information about physiological activities in various tissues and elemental transport between organs. To expand the usage of techniques, a new system was developed to visualize beta particles, x-rays gamma-rays emitted from bodies. This real-time (RRIS) visualizes radioactivity after conversion into light with CsI(Tl) scintillator plate. Herein, RRIS detection properties gamma-ray emitters 22Na, 65Zn, 86Rb, 109Cd 137Cs were evaluated...
Wild species in the genus Vigna are a great resource of tolerance to various stresses including salinity. We have previously screened genetic resources and identified several accessions that independently evolved salt tolerance. However, many aspects such remained unknown. Thus, we used autoradiography with radioactive sodium (22Na+) Inductively Coupled Plasma Mass Spectrometry (ICP-MS) visualize compare Na+ allocation angularis (Willd.) Ohwi & H.Ohashi (azuki bean), nakashimae (Ohwi)...
Cadmium (Cd) is one of the environmental pollutants contaminated in our food. Several previous reports showed that rice polishing cannot be efficient to reduce Cd content white rice, implying characteristic distribution grain. However, has not been fully elucidated so far. Herein,
Abstract Short day length-induced alteration of potassium (K) localization in perennial trees is believed to be a mechanism for surviving and adapting severe winters. To investigate the relationship between cesium (Cs) K localizations, model tree poplar, hybrid aspen T89, was employed. Under short length conditions, amount 137 Cs absorbed through root translocated drastically reduced, but 42 not. Potassium uptake from rhizosphere mediated mainly by KUP/HAK/KT CNGC transporters. In however,...
エマルション中でのDHA含有トリアシルグルセロール(TG)の酸化に対する各種タンパク質の阻害作用について検討した.DHA含有TGとしては,DHAを16%含有するTG(DHA 16%)と40%含有するTG(DHA40%)を用いた.また,タンパク質としては,鶏卵アルブミン,ミルクカゼイン,分離大豆タンパク質及び6種願の酵素分解大豆タンパク質を用いた.カゼインとアルブミンを用いて分散させたDHA 40%を除いて,タンパク質によりDHA含有TGを分散させた方が,Triton X-100を用いるよりも酸化されにくかった.大豆タンパク質分解物は,他のタンパク質よりもDHA含有TGの酸化に対してより高い阻害効果を示し,その効果は分解率の上昇に伴い増大した.大豆タンパク質分解物のDPPHラジカルに対する消去能力も,その分解率に伴って増大したので,大豆タンパク質分解物のDHA含有TG酸化に対する阻害効果は,主として,そのフリーラジカルに対する阻害作用に依存しているものと考えられた,しかし,各大豆タンパク質分解物の阻害程度は,DHA 16%とDHA...
We performed an imaging analysis of 42K in Arabidopsis using real-time radioisotope system(RRIS). First, we purified from 42Ar―42K generator. And then, characterized RRIS performance by quantitatively determining standard series. As a result, the dynamic range for was determined to be at least three orders magnitude. Next, evaluated level self-absorption organs comparing signal intensity detected and actual radioactivity gamma-counting method. There no significant difference detection...
Microautoradiography (MAR) is a conventional imaging method based on the daguerreotype. The technique used to visualize distribution of radionuclide-labeled compounds within tissue section. However, application classical MAR plant sections associated with several difficulties. In this study, we report an applicable fresh-frozen sections. Our had two features: (i) sample was kept frozen from collection radioisotope detection, making it possible fix solutes without solvent exchange; and (ii)...
The mineral composition of taro ( Colocasia esculenta (L.) Schott) was analyzed to develop a method distinguish produced in Japan and China. concentrations 15 elements (Al, Ca, Cl, Mg, Mn, Br, Co, Cr, Cs, Fe, K, Na, Rb, Sc, Zn) were assayed using instrumental neutron activation analysis. NO(3)(-), SO(4)(2-), H(2)PO(4)(-), Cl(-), malate, oxalate measured by ion chromatography. mean Na significantly differed (P < 0.01) between grown that Discriminant analysis performed identify the most...
Using high-speed scanning atomic force microscopy, we directly observed single-molecular enzymatic elongation of hyaluronan polymer chains at intervals 10 s on a mica or lipid bilayer surface, which Pasteurella multocida hyaluronic acid synthase (pmHAS) was immobilized. The reaction started by the addition both UDP-glucuronic and UDP-N-acetylglucosamine monomers. average catalytic rate constant (kcat) found to be 1.8 mer s–1 from one active enzyme physically adsorbed surface. When pmHAS...
Radioactivity levels of cesium (Cs)-134 and 137Cs in bamboo [Phyllostachys reticulata (Rupr) K. Koch] sprouts grown from April to June 2011 over a wide area (including Fukushima Prefecture) were elevated (max. 3100 Bq kg−1 fresh weight) after the Tokyo Electric Power Company, Inc. (TEPCO) Daiichi Nuclear Plant disaster March 2011. Bamboo 2012 also contained high radioactivity levels. imaging analysis harvested showed increasing concentration gradients lower parts top sprouts. The peels...
Phosphorus (P) is an essential macronutrient for plant growth. In deciduous trees, P remobilized from senescing leaves and stored in perennial tissues during winter further Annual internal recycling accumulation of are considered important strategy to support the vigorous growth trees. However, pathways seasonal re-translocation molecular mechanisms this transport have not been clarified. Here we show route visualized using real-time radioisotope imaging macro- micro-autoradiography. We...
AbstractThe levels of tritium in the atmosphere nowadays are those natural origin before nuclear test. Nuclear power stations, reprocessing plants and fusion facilities observed as a further occurrence source. Then, order to appraise influence long distance transport from continent where level is relatively high, it necessary investigate background tritium.Tritium concentrations 34 river waters 6 lake Japan were determined by low liquid scintillation measurement system combined with...