Aminoglycoside-resistant variants of Pseudomonas aeruginosa strain PAO1 were readily selected by culturing the organism in medium containing increasing concentrations gentamicin, tobramycin or amikacin until strains growing a concentration drug 128-fold greater than minimal inhibitory for sensitive parent strain. These resistant exhibited characteristics previously associated with impermeability type resistance mechanism, i.e., they grew more slowly strain, was unstable absence antibiotic,...

Since July 1973 cases of keratoconjunctivitis resembling epidemic were observed in the External Eye Disease Clinic at Moorfields Hospital; City Road, London. Adenovirus type 19 was isolated human embryonic kidney cells from 21 patients. The majority males between 20 and 40 years old. A small hospital outbreak involving six patients occurred. Clinical features disease, consisting moderate to severe follicular conjunctivitis, major subepithelial punctate keratitis, sometimes with...

One of the most important clinical obstacles in cystic fibrosis (CF) treatment is antibiotic failure due to biofilms produced by Pseudomonas aeruginosa The ability this pathogen survive eradication tobramycin and pathoadapt into a hyperbiofilm state leading chronic infections key its success. Retrospective studies have demonstrated that preventing pathoadaptation improving essential extend lives CF patients. To identify adjuvants enhance P. aeruginosa, we performed high-throughput screen...

Quorum sensing fundamentally alters the interaction of Vibrio cholerae with aquatic environments, environmental hosts, and human intestine. At high cell density, quorum-sensing regulator HapR represses not only expression cholera toxin co-regulated pilus, virulence factors essential in infection, but also synthesis polysaccharide (VPS) exopolysaccharide-based matrix required for abiotic biotic surface attachment. Here, we describe a feature V. quorum that shifts host-pathogen toward...

During the 18 months January 1975 to June 1976, 25 cases of acute herpetic follicular conjunctivitis and keratoconjunctivitis resembling adenovirus ocular infection presented in External Eye Disease Clinic, Moorfields Hospital, City Road, London. Herpes simplex virus was isolated HEp2 cells 22 patients, remaining 3 patients were identified by a minimum 4-fold rise level antiherpes antibody their blood. No from these but complement fixation test for positive 1 patient with cultural herpes...

There is evidence that measles virus infection in early life may predispose to Crohn's disease.To examine using serological methods a potential association between and predisposition disease.Forty five patients with disease forty healthy controls were studied prospectively.Clinical data recorded serum was analysed for virus, cytomegalovirus (CMV), adenovirus herpes simplex (HSV) antibody titres by complement fixation test (CFT), IgM enzyme linked immunosorbent assay (ELISA).Reciprocal CFT...

A comparison has been made of the efficiency human embryo kidney (HEK) cills, HeLa cells, and WI38 cells for isolation viruses from eyes patients suffering acute conjunctivitis or keratoconjunctivitis. From a total 99 specimens 21 adenoviruses (serotypes 3, 4, 7, 8, 13) were isolated in HEK eight 3 8) four (serotype 3) cells. Of ten herpes simplex nine recovered seven none The combination is not considered an adequate alternative to difficult-to-obtain eye.

A controlled double-blind stratified trial was carried out in a village Southern Iran to assess the efficacy of family-based intermittent therapy hyperendemic trachoma with topical oxytetracycline oily suspension twice daily for 7 days each month, or oral doxycycline 5 mg per kilogram body weight once comparison control group which received vitamin pills month. In addition all other members selected children9s families were also treated same regimen therapy. The treatment given period 1 year...

Abstract The effects of high‐speed centrifugation on the isolation herpes simplex virus (HSV) were studied. Aliquots laboratory or clinical specimens inoculated into test tubes and flat‐bottomed containing HEp2 monolayers. Test incubated at 35°C roller drums (standard method), centrifuged 15,000g for 1 hr, before being without rolling (centrifuged method). Centrifugation HSV type 2 produced significantly increased rates compared with standard method. When diluted, method was more sensitive...

The prevalence of chlamydial genital infection was studied in 177 prostitutes Iran; 100 Teheran and 77 the port Bandar Abbas. Chlamydia trachomatis isolated eight (6.9%) 116 patients with valid cultures. Type-specific antibodies were found against C serotypes D to K (genital serotypes) 94.2% A (trachoma 2% prostitutes. IgM at a titre greater than or equal 8, indicating current infection, 29.2%, whereas type-specific IgG 64, suggesting recent present 71.5%. lower isolation rate these women...

To examine how bacterial biofilms, as contributing factors in the delayed closure of chronic wounds patients with diabetes, affect healing process.We used daily microscopic imaging and IVIS Spectrum vivo system to monitor biofilm infections bioluminescent Pseudomonas aeruginosa evaluate non-diabetic streptozotocin-induced diabetic mice.Our studies determined that diabetes alone did not rate full-depth murine back compared mice. The application mature biofilms significantly decreased...

Although the mechanism by which cyclic AMP receptor protein (CRP) regulates global gene transcription has been intensively studied for decades, new discoveries remain to be made. Here, we report that, during rapid growth, CRP associates with both well-conserved, dual-function DNA-binding peptidase A (PepA) and cell membrane. These interactions are not present under nutrient-limited growth conditions, due post-translational modification of three lysines on a single face CRP. coincident DNA...

A case of keratoconjunctivitis caused by adenovirus type 21 in London has been described. 59-year-old woman attented hospital August 1974 complaining a 3-week history redness, grittiness, watery discharge, and photophobia her left eye slight upper respiratory infection. Clinical examination showed moderate follicular conjuctivitis mainly the lower fornices, which lasted for 6 weeks. In cornea amount epithelial subepithelial punctate keratitis was observed. The opacities were coarse,...

The presence of bacteria as structured biofilms in chronic wounds, especially diabetic patients, is thought to prevent wound healing and resolution. Chronic mouse wounds models have been used understand the underlying interactions between microorganisms host. developed date rely on use haired animals terminal collection tissue for determination viable bacteria. While significant insight has gained with these models, this experimental procedure requires a large number sampling time consuming....

The sensitivity of human embryonic kidney (HEK) cell culture and the complement fixation test (cft) in diagnosis adenovirus ocular infection has been compared. optimum time for collection specimens to obtain best results by each examined. Sixty-one (53%) 116 patients with moderate severe follicular conjunctivitis, clinically indicative infection, had serological evidence or virus isolated from swabs. Virus was 53 (45%) significant antibody rises were found 33 (27%). When carried out under...

Objective: To investigate the association of cadmium exposure with liver function among adults in a non-ferrous metal mining area Guangxi. Methods: A total 310 residents aged 18 and above were recruited from 5 heavy metals polluted villages Guangxi 2013 to 2014. The general demographic characteristics, blood levels indicators index [Total bilirubin (TBIL), Glutamic oxaloacetic transaminase (AST), Alanine (ALT) Glutamine (GGT)] obtained by using questionnaire, physical examination laboratory...

The presence of bacteria as structured biofilms in chronic wounds, especially diabetic patients, is thought to prevent wound healing and resolution. Chronic mouse wounds models have been used understand the underlying interactions between microorganisms host. developed date rely on use haired animals terminal collection tissue for determination viable bacteria. While significant insight has gained with these models, this experimental procedure requires a large number sampling time consuming....

Diarrheal disease is the most common infectious of children in developing world. Our goal to develop a diarrheal antigen presentation platform based on whole Vibrio cholerae cells that does not depend protein purification. We have previously shown feasibility genetically fusing antigens V. biofilm matrix RbmA for cell surface. A mucosal adjuvant could improve immunogenicity such vaccine at Here we engineer live attenuated constitutively synthesize mmCT, nontoxic form cholera toxin. When this...