A5035706913- Retinal Diseases and Treatments
- Cancer-related Molecular Pathways
- Effects of Radiation Exposure
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer, Hypoxia, and Metabolism
- Advanced Glycation End Products research
- Respiratory and Cough-Related Research
- Asthma and respiratory diseases
- Cancer Research and Treatments
- Carcinogens and Genotoxicity Assessment
- Wound Healing and Treatments
- PI3K/AKT/mTOR signaling in cancer
- Bacterial biofilms and quorum sensing
- DNA Repair Mechanisms
- Kruppel-like factors research
- Genomics and Chromatin Dynamics
- Metabolomics and Mass Spectrometry Studies
- Rheumatoid Arthritis Research and Therapies
- NF-κB Signaling Pathways
- Cell death mechanisms and regulation
- Peroxisome Proliferator-Activated Receptors
- Antimicrobial Peptides and Activities
- Retinal Imaging and Analysis
- Metabolism and Genetic Disorders
- Protein Tyrosine Phosphatases
Michigan State University
2007-2024
Michigan United
2016-2022
Sp1 is a transcription factor for many genes, including genes involved in tumorigenesis. We found that human fibroblast cells malignantly transformed culture by carcinogen or stable transfection of an oncogene express at 8-fold to 18-fold higher levels than their parental cells. These cell lines form fibrosarcomas athymic mice with very short latency, and the from tumors same high Sp1. Similar were patient-derived fibrosarcoma tested, formed these lines. To investigate role overexpression...
The purpose of this study was to investigate the antitussive activity and sites action NK 1 2 tachykinin receptor antagonists, CP‐99,994, SR 48968, racemate 48212A in cat guinea‐pig. Guinea‐pigs were dosed subcutaneously (s.c.) with or 48968 one hour before exposure aerosols capsaicin (0.3 m M ) elicit coughing. Coughs detected a microphone counted. Intracerebroventricular (i.c.v.) cannulae placed lateral cerebral ventricles anaesthetized guinea‐pigs. Approximately week later, animals...
The GABA‐B receptor agonists baclofen and 3‐aminopropylphosphinic acid (3‐APPi) have antitussive activity in the cat guinea pig. purpose of this study was to investigate sites action these inhibit cough reflex. Single intracerebroventricular (i.e.v.) cannulas were placed lateral ventricles anaesthetized pigs. Approximately 1 week later, animals exposed aerosols capsaicin (0.3 μ m ) elicit coughing. Coughs detected with a microphone counted. Cough produced cats by mechanical stimulation...
Magnetic nanoparticles are attractive platforms for biomedical applications including diagnosis and treatment of diseases. We have shown previously that hyaluronan-coated superparamagnetic iron oxide (HA-SPIONs) enhanced the efficacy conjugated anticancer drug doxorubicin (DOX) in vitro against drug-sensitive drug-resistant human ovarian cancer cells. In this manuscript, we report our findings on DOX loaded HA-SPIONs vivo using subcutaneous intraperitoneal SKOV-3 tumor models nude mice. The...
Abstract The metabolic insults associated with diabetes lead to low-grade chronic inflammation, retinal endothelial cell damage, and inadequate vascular repair. This is partly due the increased activation of bone marrow (BM)-derived proinflammatory monocytes infiltrating retina, compromised function BM-derived reparative circulating angiogenic cells (CACs), which home sites injury foster We now propose that a link leading activated dysfunctional CACs in involves upregulation central enzyme...
Tight junctions (TJs) involve close apposition of transmembrane proteins between cells. Although TJ have been studied in detail, the role lipids is largely unknown. We addressed very long-chain (VLC ≥26) ceramides TJs using diabetes-induced loss blood-retinal barrier as a model. VLC fatty acids that incorporate into are produced by elongase elongation protein 4 (ELOVL4). ELOVL4 significantly reduced diabetic retina. Overexpression decreased basal permeability, inhibited vascular endothelial...
Diabetic retinopathy is a sight-threatening complication of diabetes, affecting 65% patients after 10 years the disease. metabolic insult leads to chronic low-grade inflammation, retinal endothelial cell loss and inadequate vascular repair. This partly due bone marrow (BM) pathology leading increased activity BM-derived pro-inflammatory monocytes impaired function reparative circulating angiogenic cells (CACs). We propose that diabetes has significant long-term effect on nature proportion...
To investigate the relationship between two well-established delayed effects of ionizing radiation, experiments were conducted to determine induction and expression lethal mutations (delayed reproductive death) chromosomal instability with respect dose time in a human immortalized keratinocyte cell line.HPV-G cells gamma- or alpha-irradiated maintained culture for up 72 population doublings. At intervals, measurements made cloning efficiency examined apoptosis cytogenetic aberrations.The...
Abstract Hypoxia is a common feature of solid tumors. The cellular response to hypoxic stress controlled by family prolyl hydroxylases (PHD) and the transcription factor hypoxia-inducible 1 (HIF1). To investigate relationship between PHD HIF1 activity transformation, we characterized expression levels isoforms across lineage cell strains with varying transformed characteristics. We found that PHD2 primary functional isoform in these cells its are inversely correlated tumor-forming potential....
Bile acids (BAs) are steroid detergents in bile that contribute to fat absorption, cell signaling, and microbiome interactions. The final step their synthesis is amino acid conjugation with either glycine or taurine the liver by enzyme acid-CoA:amino N-acyltransferase (BAAT). Here, we describe microbial, chemical, physiological consequences of Baat gene knockout. Baat-/- mice were underweight after weaning but quickly exhibited catch-up growth. At three weeks age, KO animals had increased...
// Inez Yuwanita 1 , Danielle Barnes Michael D. Monterey Sandra O'Reilly Eran R. Andrechek Department of Physiology, Michigan State University, East Lansing, MI48824, Michigan, USA Correspondence to: Andrechek, e-mail: andrech1@msu.edu Keywords: MMTV-Myc, metastasis, E2F transcription factors, PTPRD, gene expression Received: June 01, 2015 Accepted: September 30, Published: October 13, 2015 ABSTRACT In human breast cancer, mortality is associated with metastasis...
Sprouty 2 (Spry2) acts as an inhibitor of receptor tyrosine kinase signaling in various cellular contexts. Interestingly, Spry2 also prevents the c-Cbl-induced degradation epidermal growth factor (EGFR). We compared human fibroblasts malignantly transformed by overexpression H-Ras(V12) oncogene to their nontransformed parental cells and found that malignant express a high level Spry2. These exhibited increase EGFR with precursor cells. intact was required if H-Ras-transformed were grow...
The quantification of the extent delayed cell death and rate pattern its occurrence in relation to division cycle is important radiotherapy also radiation transformation studies related protection dose limits. Here numbers lethal mutations occurring over 45 population doublings (clonal expansion about 1013 cells per originally surviving irradiation) was measured an HPV 16 immortalized human keratinocyte lines used for studies. results showed that when postirradiation (dose range 1–6 Gy)...
The role of EGFR in lung cancer is well described with numerous activating mutations that result phosphorylation and tyrosine kinase inhibitors target EGFR. While the non-small cell (NSCLC) appreciated, control signaling pathways through dephosphorylation by phosphatases not as clear. Through whole genome sequencing we have uncovered conserved V483M Ptprh PyMT induced tumors. Profiling downstream events mutant tumors revealed AKT activation, suggesting a key PTPRH was 1197. Given cancer,...
We studied the oral actions of antihistamines from six chemical classes, namely: ethanolamines (ENA, diphenhydramine and clemastine); ethylenediamines (EDA, pyrilamine tripelennamine); piperidines (PPD, terfenadine astemizole); piperazines (PPZ, hydroxyzine cetirizine); phenothiazines (PTZ, promethazine), alkylamines (ALA, chlorpheniramine bromopheniramine) on cough reflexes, pentobarbital-induced sedation minute ventilation in conscious guinea pig. Antihistamines ENA class had minimal...
Changes in expression of hepatocyte growth factor (HGF) and its receptor, MET, are associated with formation malignant progression human tumors. In the present study, 10 11 fibrosarcoma cell lines tested expressed significantly higher levels MET than were found a series normal fibroblast lines. Still more significant, was constitutively phosphorylated all lines, whereas fibroblasts exhibited very low form. All HGF mRNA. To determine role and/or tumorigenesis, line expressing high protein...
Abstract Background The activities of Rac1 and Cdc42 are essential for HRas-induced transformation rodent fibroblasts. What is more, expression constitutively activated mutants and/or sufficient their malignant transformation. role these two Rho GTPases in HRas-mediated human fibroblasts has not been studied. Here we evaluated the contribution to maintaining fibroblasts, determined ability or induce a fibroblast cell strain. Methods Under control tetracycline regulatable promoter, dominant...
To examine how bacterial biofilms, as contributing factors in the delayed closure of chronic wounds patients with diabetes, affect healing process.We used daily microscopic imaging and IVIS Spectrum vivo system to monitor biofilm infections bioluminescent Pseudomonas aeruginosa evaluate non-diabetic streptozotocin-induced diabetic mice.Our studies determined that diabetes alone did not rate full-depth murine back compared mice. The application mature biofilms significantly decreased...
Recent evidence from our laboratory suggests that the fraction of cells with lethal mutations is lost population by apoptosis. The relationship this process to genetic instability and carcinogenesis unclear. To examine this, tumorigenic cell populations derived spontaneously occurring, neoplastically transformed C3H 1OT1/2 foci radiation-induced were compared wild-type 10T1/2 determine frequency induction postirradiation. Lethal did not occur in progeny type 3 cultures occurring or but very...
ABSTRACT Bile acids are steroid detergents in bile that contribute to fat absorption, cell signaling and microbiome interactions mammals. The final step their synthesis is amino acid conjugation with either glycine or taurine a cholic chenodeoxycholic backbone the liver by enzyme acid-CoA:amino N-acyltransferase (BAAT). Here, we describe microbial, chemical, physiological consequences of BAAT gene deletion mice. -/- mice were underweight after weaning but quickly exhibited catch-up growth....
The presence of bacteria as structured biofilms in chronic wounds, especially diabetic patients, is thought to prevent wound healing and resolution. Chronic mouse wounds models have been used understand the underlying interactions between microorganisms host. developed date rely on use haired animals terminal collection tissue for determination viable bacteria. While significant insight has gained with these models, this experimental procedure requires a large number sampling time consuming....