A5042933149- Gut microbiota and health
- Drug Transport and Resistance Mechanisms
- Cystic Fibrosis Research Advances
- Diet and metabolism studies
- Pediatric health and respiratory diseases
- Orthopedic Infections and Treatments
- Metabolomics and Mass Spectrometry Studies
- Epigenetics and DNA Methylation
- Pharmacological Effects and Toxicity Studies
- Peroxisome Proliferator-Activated Receptors
- Clostridium difficile and Clostridium perfringens research
- Metabolism and Genetic Disorders
- Bacterial biofilms and quorum sensing
- Probiotics and Fermented Foods
- Ginger and Zingiberaceae research
- Vitamin D Research Studies
- Respiratory viral infections research
- Orthopaedic implants and arthroplasty
- Antimicrobial Resistance in Staphylococcus
- Respiratory and Cough-Related Research
- Diet, Metabolism, and Disease
- Microbial Metabolism and Applications
- Gastroesophageal reflux and treatments
- Wound Healing and Treatments
- Pediatric Hepatobiliary Diseases and Treatments
Michigan State University
2021-2025
The Ohio State University
2020
Periprosthetic joint infections (PJIs) are a devastating complication that occurs in 2% of patients following replacement. These costly and difficult to treat, often requiring multiple corrective surgeries prolonged antimicrobial treatments. The Gram-positive bacterium Staphylococcus aureus is one the most common causes PJIs, it resistant number commonly used antimicrobials. This tolerance can be partially attributed ability S. form biofilms. Biofilms associated with surface indwelling...
Elexacaftor-Tezacaftor-Ivacaftor (ETI) therapy is showing promising efficacy for treatment of cystic fibrosis (CF) and becoming more widely available since recent FDA approval. However, little known about how these drugs will affect lung infections, which are the leading cause morbidity mortality among people with CF (pwCF).
Abstract Antibiotics are our primary approach to treating complex infections, yet we have a poor understanding of how these drugs affect microbial communities. To better understand antimicrobial effects on host-associated communities treated cultured sputum microbiomes from people with cystic fibrosis (pwCF, n = 24) 11 different antibiotics, supported by theoretical and mathematical modeling-based predictions in mucus-plugged bronchiole microcosm. Treatment outcomes identified vitro that...
Bile acids (BAs) are steroid detergents in bile that contribute to fat absorption, cell signaling, and microbiome interactions. The final step their synthesis is amino acid conjugation with either glycine or taurine the liver by enzyme acid-CoA:amino N-acyltransferase (BAAT). Here, we describe microbial, chemical, physiological consequences of Baat gene knockout. Baat-/- mice were underweight after weaning but quickly exhibited catch-up growth. At three weeks age, KO animals had increased...
Cystic fibrosis (CF) is a genetic disorder causing poor mucociliary clearance in the airways and subsequent respiratory infection. The recently approved triple therapy Elexacaftor-Tezacaftor-Ivacaftor (ETI) has significantly improved lung function decreased airway infection persons with CF (pwCF). This improvement been shown to occur rapidly, within first few weeks of treatment. effects longer term ETI on dynamics, however, remain mostly unknown.
Abstract Bile acids (BAs) are steroid detergents in bile that contribute to the absorption of fats and fat-soluble vitamins while shaping gut microbiome due their antimicrobial properties. Recently, a novel mechanism BA metabolism by microbiota was identified involving conjugation with various amino acids. Here, we show this unique biochemistry is mediated salt hydrolase acting as an aminoacyltransferase or transpeptidase (BSH/T). Clostridium perfringens BSH/T rapidly performed acyl-transfer...
It is well established in the microbiome field that antibiotic (ATB) use and metabolic disease both impact structure function of gut microbiome. But how host microbial metabolism interacts with ATB susceptibility to affect resulting dysbiosis remains poorly understood. In a streptozotocin-induced model hyperglycemia (HG), we combined metagenomic, metatranscriptomic, metabolomic approach profile changes taxonomic composition, transcriptional activity, metabolite abundance pre- post-ATB...
Glucocorticoid-induced osteoporosis (GIO) is a significant side effect of prolonged glucocorticoid (GC) treatment. Chronic GC treatment also leads to trabecular bone loss and gut microbiota dysbiosis in mice. The mechanistically linked GIO, which indicates that the can be targeted prevent GIO. Prunes, dried fruit prebiotic, have emerged literature as an effective for sex-steroid deficiency induced (primary osteoporosis). Prunes significantly alter composition both rodent models human...
While enteric pathogens have been widely studied for their roles in causing foodborne infection, impacts on the gut microbial community yet to be fully characterized. Previous work has identified notable changes microbiome related pathogen invasion, both taxonomically and genetically. Characterization of metabolic landscape during after however, not explored. Consequently, we investigated metabolome paired stools recovered from 60 patients (cases) recovery bacterial infections (follow-ups)....
ABSTRACT Bile acids are steroid detergents in bile that contribute to fat absorption, cell signaling and microbiome interactions mammals. The final step their synthesis is amino acid conjugation with either glycine or taurine a cholic chenodeoxycholic backbone the liver by enzyme acid-CoA:amino N-acyltransferase (BAAT). Here, we describe microbial, chemical, physiological consequences of BAAT gene deletion mice. -/- mice were underweight after weaning but quickly exhibited catch-up growth....
<title>Abstract</title> Conjugated bile acids (BAs) are multi-functional detergents produced in the gastrointestinal (GI) tract by liver enzyme acid:amino acid N-acyltransferase (BAAT) and microbiome from acyltransferase activity of ubiquitous salt hydrolase (BSH). Humans with inflammatory bowel disease (IBD) have an enrichment both host microbially conjugated BAs (MCBAs), but their impacts on GI inflammation not well understood. We investigated role host-conjugated dextran sodium sulfate...
Population studies have shown that the infant's microbiome and metabolome undergo significant changes in early childhood. However, no previous study has investigated how diverse these are across subjects whether subject-specific dynamics of some microbes correlate with over-time specific metabolites. Using mixed-effects models, data from ABC study, we childhood fecal identified 83 amplicon sequence variants (ASVs) 753 metabolites seemingly coordinated trajectories. Enrichment analysis...
Cystic fibrosis (CF) is a genetic disorder causing poor mucociliary clearance in the airways and subsequent respiratory infection. The recently approved triple therapy Elexacaftor-Tezacaftor-Ivacaftor (ETI) has significantly improved lung function decreased airway infection of persons with CF (pwCF). This improvement been shown to occur rapidly, within first few weeks treatment. effects longer term ETI on dynamics, however, remains mostly unknown.Here, we applied 16S rRNA gene amplicon...
Abstract Background Novel small molecule therapies for cystic fibrosis (CF) are showing promising efficacy and becoming more widely available since recent FDA approval. The newest of these is a triple therapy Elexacaftor-Tezacaftor-Ivacaftor (ETI, Trikafta®). Little known about how drugs will affect polymicrobial lung infections, which the leading cause morbidity mortality among people with CF (pwCF). Methods we analyzed sputum microbiome metabolome from pwCF (n=24) before after ETI using...