We have demonstrated chromosomal instability in the clonal descendants of hemopoietic stem cells after irradiating murine bone marrow with α-particles. However, because that are irradiated by α-particles defined a Poisson distribution individual particle traversals, there is an inevitable proportion unirradiated surviving population. The calculated expected proportions and nonirradiated indicate number clonogenic transmitting greater than to be hit survive. To investigate further this...

Transmissible chromosomal instability, characterized by non-clonal cytogenetic aberrations with a high frequency of chromatid-type together lower chromosome-type aberrations, has been demonstrated in the clonal descendants human haemopoietic stem cells after alpha- but not X-irradiation. Comparable abnormalities have also cultures alpha-irradiated primary bone marrow, different pattern delayed mainly chromosome-type, was found X-irradiation cultures. In analyses, apoptotic cell death evident...

Oral anticancer medicines are frequently administered to patients with cancer. Regrettably, many medications without appropriate recommendations about handling practices. This study aims assess the handling, storage, and disposal practices of oral medication among cancer at home. A cross-sectional was conducted from December 2023 February 2024 on adult who were Hematology Bone Marrow Transplant Center in Iraq using pre-validated questionnaire. In total, 300 interviewed, (57%) being male...

Background: Renal stones also called nephrolithiasis or urolithiasis, is a medical issue involves the formation and sometimes passage of crystalline within urinary tract. The aim present study to assess lifestyle dietary factors associated with renal in Duhok City. Methods: A cross-sectional design was conducted, data were collected during period from September 1, 2024, December 31, 2024. convenience sample patients who admitted Urology Ward visited Consultation Unit at Azadi Teaching...

Previous studies detected both lethal and cumulative chromosomal aberrations in bone marrow peripheral blood of patients with worn hip knee replacements. This study shows that wear debris from a titanium metal on high-density polyethylene replacement also produces instability reproductive failure cell culture. The progeny these treated cells displayed instability, mainly consisting chromatid breaks minutes, as determined by clonogenic survival many generations postexposure. These delayed...

Kadhim, M. A., Marsden, S. J., Malcolmson, A. M., Folkard, Goodhead, D. T., Prise, K. and Michael, B. Long-Term Genomic Instability in Human Lymphocytes Induced by Single-Particle Irradiation.Recent evidence suggests that genomic instability, which is an important step carcinogenesis, may be the effectiveness of radiation as a carcinogen, particularly for high-LET radiations. Understanding biological effects underpinning risks associated with low doses densely ionizing radiations complicated...

To investigate the relationship between two well-established delayed effects of ionizing radiation, experiments were conducted to determine induction and expression lethal mutations (delayed reproductive death) chromosomal instability with respect dose time in a human immortalized keratinocyte cell line.HPV-G cells gamma- or alpha-irradiated maintained culture for up 72 population doublings. At intervals, measurements made cloning efficiency examined apoptosis cytogenetic aberrations.The...

The target theory of radiation-induced effects has been challenged by numerous studies, which indicate that in addition to biological resulting from direct DNA damage within the cell, a variety non-DNA targeted (NTE) may make important contributions overall outcome. Ionising radiation induces complex, global cellular responses, such as genomic instability (GI) both irradiated and never-irradiated 'bystander' cells receive molecular signals produced cells. GI is well-known feature many...

Studying the potential role of tumour necrosis factor (TNF)alpha in initiation genomic instability is necessary to understand whether TNFalpha can serve as a signalling mediator radiation-induced non-irradiated bystander cells. In this study, we examined could initiate processes through oxidative stress that lead DNA damage and primary vascular endothelium. these cells, low linear energy transfer (LET) radiation (0.1-2 Gy) induced secretion into culture medium. When added ectopically, at...

Previous work has demonstrated that alpha-particle irradiation of primary human bone marrow cells leads to the transmission chromosomal instability in descendants irradiated cell, although there is some interindividual variation. We have extended these studies EBV-transformed lymphoblastoid cell lines order establish an vitro model system. The five analyzed, including one from a Fanconi anaemia patient, exhibited high levels persistent chromatid aberrations up ~40 generations after...

If radiation-induced genetic instability is causal in mouse radiation leukaemogenesis, then should be detectable the irradiated target untransformed haemopoietic stem cell, and evidence of detected clonal leukaemia. We have tested this hypothesis using CBA/H model acute myeloid leukaemia (r-AML).Fluorescence situ hybridisation (FISH) was employed to screen for chromosomal aberrations 3 Gy X-ray-induced r-AMLs descendents control X-irradiated bone marrow cells vitro clonogenic CFU-A colony...

Abstract At Lund University and Skåne Hospital in Lund, Sweden, we have, as the first clinic, modified a clinical Elekta Precise linear accelerator for convertible delivery of ultra-high dose rate (FLASH) irradiation. Whereas recently published reviews highlighted need standardised protocols beam dosimetry to be able determine true potential FLASH irradiation, several studies well in-vitro in-vivo experiments have been carried out at our unit. Dosimetric procedures verification accurate...

Complex detection in protein-protein interaction (PPI) networks is one of the major issues facing scientific study biological networks. In PPINs, proteins are distributed differently as groups (complexes). These can be identified having a great internal density number edges inside while least possible between these groups. The most common methods for finding such complexes evolutionary algorithms (EAs), which have been used widely literature this objective. Despite reliability complicated...

A robust method for obtaining chromosome preparations from individual haemopoietic colonies in semi‐solid media is described. The accumulation of metaphases and the hypotonic treatment cells were carried out culture dish transferred onto poly‐ l ‐lysine‐treated slides fixed stepwise prior to staining. technique produced high yields well‐spread facilitating clonal cytogenetic analysis.

Stable-type chromosome aberrations that survive multiple generations of cell division include translocation and inversions. An efficient method to detect an inversion is multi-color banding fluorescent in situ hybridization (mBAND) which allows identification both inter- intra-chromosome simultaneously. Post irradiation, may also arise after divisions as a result genomic instability. This study was aimed at investigating stable or late-arising human lymphocytes induced low- high-LET...