A5073040565- Immunotherapy and Immune Responses
- Cancer Cells and Metastasis
- Cancer Immunotherapy and Biomarkers
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Atherosclerosis and Cardiovascular Diseases
- Immune cells in cancer
- CAR-T cell therapy research
- Cancer Research and Treatments
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Viral Infections and Outbreaks Research
- Viral Infections and Vectors
- Cellular Mechanics and Interactions
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Mosquito-borne diseases and control
- Cholesterol and Lipid Metabolism
Universidade Nova de Lisboa
2017-2023
Instituto Politécnico de Lisboa
2023
Universidade do Porto
2018
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2016-2018
Institut Pasteur
2018
IPO Porto
2018
Abstract Epithelial-to-mesenchymal transitions (EMT) are strongly implicated in cancer dissemination. Intermediate states, arising from inter-conversion between epithelial (E) and mesenchymal (M) characterized by phenotypic heterogeneity combining E M features increased plasticity. Hybrid EMT states highly relevant metastatic contexts, but have been largely neglected, partially due to the lack of physiologically-relevant 3D platforms study them. Here we propose a new vitro model, mammary...
Prediction of breast cancer response to Neoadjuvant Chemotherapy (NACT) is an urgent need promptly direct non-responder patients alternative therapies. Infiltrating T lymphocytes, namely cytotoxic lymphocytes (CTLs) have been appointed as predictors response. However, cells the ability dampen CTLs' activity and thus, prognostic value CTLs, per se, debatable. Here, we disclose that more than occurrence it their activation state, revealed by HLA-DR expression, can accurately predict NACT. Flow...
3D cell culture including different types, such as immune cells, is a representative platform that mimics the tumor microenvironment. Here we disclose an easy-to-handle co-culture protocol using scaffold-free technique with breast cancer line MDA-MB-231 and patient-derived cells from peripheral blood. The method presented simple, less time-consuming expensive when compared to other techniques. Additionally, this optimized for establishment of system line, which normally seen challenging...
Neutrophils are prominent immune components of tumors, having either anti-tumor (N1) or pro-tumor activity (N2). Circulating neutrophils, divided into high density neutrophils (HDN) and low (LDN), functionally mirror those N1 N2 cells, respectively. LDN rare in non-pathological conditions, but frequent cancer, exhibiting a phenotype. These findings have been mainly demonstrated animal models, thus proper validation humans is still imperative. Here, we observed that were increased the blood...
Neoadjuvant chemotherapy (NACT) is common in breast cancer (BC) treatment, though more than half of the patients lack an effective response. Therefore, new predictive biomarkers and alternative therapies are crucial. Previously, we proposed HLA-DR-expressing cytotoxic T lymphocytes (CTLs) as a potential biomarker response to NACT. To validate this observation further investigate these cells, 202 BC were enrolled. Flow cytometry analyses performed 61 biopsies 41 blood samples pre-NACT 100...
Oxidation of PUFAs in LDLs trapped the arterial intima plays a critical role atherosclerosis. Though there have been many studies on atherogenicity oxidized derivatives PUFA-esters cholesterol, effects cholesteryl hemiesters (ChEs), oxidation end products these esters, not studied. Through lipidomics analyses, we identified and quantified two ChE types plasma CVD patients four human endarterectomy specimens. Cholesteryl hemiazelate (ChA), azelaic acid (n-nonane-1,9-dioic acid), was most...
Abstract Neutrophils are prominent immune components of solid tumors, which can protect against the onset cancer (N1) or have pro-tumor activity (N2). Circulating neutrophils, divided into high density neutrophils (HDN) and low (LDN), functionally mirror those N1 N2 cells, respectively. LDN, a rare subset in non-pathological conditions, been extensively studied cancer, due to their frequency this disease phenotype. However, has mainly demonstrated animal models proper validation humans is an...
ABSTRACT Oxidation of polyunsaturated fatty acids (PUFA) in low-density lipoproteins (LDL) trapped the arterial intima plays a critical role atherosclerosis. Though there have been many studies on atherogenicity oxidized derivatives unsaturated acid esters cholesterol, effects oxidation end-products these has ignored literature. Through lipidomics analyses plasma cardiovascular disease patients and human endarterectomy specimens we identified quantified cholesteryl hemiesters (ChE),...
Abstract Background Neoadjuvant chemotherapy (NACT) is the usual treatment for locally-advanced breast cancer (BC). However, more than half of patients lack an effective response to this treatment. Thus, it’s urgent find predictive biomarkers. Recently, we proposed HLA-DR expression level in cytotoxic T lymphocytes (CTLs) as a robust biomarker select, advance, that will actually benefit from NACT. Patients and Methods A total 202 BC patients, 102 which submitted NACT, were enrolled study. 61...