A5061720887- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- Vector-Borne Animal Diseases
- Respiratory viral infections research
- Plant Virus Research Studies
- Congenital Anomalies and Fetal Surgery
- Animal Disease Management and Epidemiology
- Microbial infections and disease research
- Monoclonal and Polyclonal Antibodies Research
- Animal Genetics and Reproduction
- Escherichia coli research studies
- Viral Infectious Diseases and Gene Expression in Insects
- Glycosylation and Glycoproteins Research
- T-cell and Retrovirus Studies
- Urinary Tract Infections Management
- Energy Harvesting in Wireless Networks
- Antibiotic Resistance in Bacteria
- Parasitic Infections and Diagnostics
- Bacterial biofilms and quorum sensing
- Biosensors and Analytical Detection
- Microbial Community Ecology and Physiology
- Advanced Thermodynamic Systems and Engines
Baylor College of Medicine
2011-2022
Stanford University
2011
University of Miami
1994-1996
Virologie et Immunologie Moléculaires
1994
Texas A&M University
1989-1993
Texas Medical Center
1983
Harvard University
1977-1980
Brigham and Women's Hospital
1977
The continued rise in antibiotic resistance is precipitating a medical crisis. Bacteriophage (phage) has been hailed as one possible therapeutic option to augment the efficacy of antibiotics. However, only few studies have addressed synergistic relationship between phage and Here, we report comprehensive analysis phage-antibiotic interaction that evaluates synergism, additivism, antagonism for all classes antibiotics across clinically achievable stoichiometries. We combined an optically...
The virus-specific double-stranded genome RNA and polypeptides present in virions cells infected with the three mammalian reovirus serotypes have been examined by co-electrophoresis several different polyacrylamide gel systems. polypeptide species previously described for type 3 Dearing were found to corresponding other examined. In each serotype electrophoretic mobilities from or of Dearing. combination variants among RNAs gave markers all 10 genes. usefulness these "mapping" is discussed.
Phage therapy is a promising treatment of multi-drug resistant (MDR) bacterial infections but limited by the narrow host range phage. To overcome this limitation, we developed expansion (HRE) protocol that expands Pseudomonas aeruginosa-specific phage cycles co-incubation with multiple P. aeruginosa strains. Application HRE to mixture 4 phages, using 16 strains for development, resulted in undefined mixtures greatly expanded range. Individual clones derived from had ranges no individual...
Phage therapy requires libraries of well-characterized phages. Here we describe the generation phage for three target species: Escherichia coli, Pseudomonas aeruginosa, and Enterobacter cloacae. The basic characteristics on isolation host, sequence analysis, growth properties, host range virulence a number contemporary clinical isolates are presented. This information is required before phages can be added to library potential human use or sharing between laboratories in compassionate...
ABSTRACT Trypsin enhances rotavirus infectivity by an unknown mechanism. To examine the structural basis of trypsin-enhanced in rotaviruses, SA11 4F triple-layered particles (TLPs) grown absence (nontrypsinized [NTR]) or presence (trypsinized [TR]) trypsin were characterized to determine structure, protein composition, and before after treatment. As expected, VP4 was not cleaved NTR into VP5 ∗ VP8 TR particles. However, surprisingly, while spikes clearly visible well ordered electron...
Seven-day-old CD-1 mice born to seronegative dams were orally inoculated with a mixture of wild-type simian rotavirus SA11 and rhesus RRV. At various times postinfection, progeny clones randomly isolated from intestinal homogenates by limiting dilution. Analysis genome RNAs polyacrylamide gel electrophoresis was used identify genotype reassortant progeny. Reassortment segments observed in 252 662 (38%) analyzed vivo mixed infections. Kinetic studies indicated that reassortment an early event...
Naturally occurring bovine-human reassortant rotaviruses with a P[11] VP4 genotype exhibit tropism for neonates. Interaction of the VP8* domain spike protein sialic acid was thought to be key mediator rotavirus infectivity. However, recent studies have indicated role nonsialylated glycoconjugates, including histo-blood group antigens (HBGAs), in infectivity human rotaviruses. We sought determine if bovine rotavirus-derived neonatal G10P[11] virus interacts hitherto uncharacterized glycans....
Invasive pathobionts or microbes capable of causing disease can reside deep within the mucosal epithelium our gastrointestinal tract. Targeted effective antibacterial therapies are needed to combat these disease-causing organisms, many which may be multidrug resistant.
ABSTRACT Virus-like particles (VLPs) are being evaluated as a candidate rotavirus vaccine. The immunogenicity and protective efficacy of different formulations VLPs administered parenterally to rabbits were tested. Two doses (2/6-, G3 2/6/7-, or P[2], 2/4/6/7-VLPs) SA11 simian in Freund’s adjuvants, QS-21 (saponin adjuvant), aluminum phosphate (AlP) administered. Serological mucosal immune responses all vaccinated control before after oral challenge with 10 3 50% infective live P[14], ALA...
Summary The primary vector species for bluetongue virus (BTV) in the United States, Culicoides variipennis, was orally infected with BTV serotype 10, 17, or a mixture of two viruses. recovery from flies low following period extrinsic incubation. Electrophoretic analysis progeny singly revealed that only parental electropherotype could be isolated those flies. In contrast, electrophoretic mixedly eight 11 virus-positive produced reassortant electropherotypes. proportion varied 7 to 78% (mean...
Two seronegative sheep were infected intravenously with 10(9) PFU each of bluetongue virus (BTV) serotype 10 and BTV 17. One animal experienced a mild bluetongue-like disease, both short-duration viremia developed neutralizing immune responses to serotypes. Progeny was isolated from venous blood by using conditions in which reassortment could not have occurred during isolation. Electropherotypes determined for the progeny viruses sheep, yielding strikingly similar results two animals. In...
Abstract Multi-drug resistant (MDR) enteric bacteria are of increasing global concern. A clonal group, Escherichia coli sequence type (ST) 131, harbors both MDR and a deadly complement virulence factors. Patients with an immunocompromised system at high risk infections these E. there is strong epidemiologic evidence that the human intestinal tract, as well household pets, may be reservoir. Here, we examine if phages effective treatment strategy against this group in murine models bacteremia...
High rates of antimicrobial resistance and formation biofilms makes treatment Escherichia coli catheter-associated urinary tract infections (CAUTI) particularly challenging. CAUTI affect 1 million patients per year in the United States are associated with morbidity mortality, as an etiology for sepsis. Phage have been proposed a potential therapeutic option. Here, we report development phage cocktails that lyse contemporary E. strains isolated from urine spinal cord injury (SCI) display...
Rotavirus has a capsid composed of three concentric protein layers. We coexpressed various combinations the rotavirus structural proteins single-layered (core) and double-layered (single-shelled) capsids from baculovirus vectors in insect cells determined ability to assemble into viruslike particles (VLPs). VLPs were purified by centrifugation, their structure was examined negative-stain electron microscopy, content sodium dodecyl sulfate-polyacrylamide gel electrophoresis GTP binding...
A template-dependent, in vitro rotavirus RNA replication system was established. The initiated and synthesized full-length double-stranded RNAs on positive-sense template RNAs. Native mRNAs or transcripts, with bona fide 3' 5' termini, derived from cDNAs functioned as templates. Replicase activity associated a subviral particle containing VP1, VP2, VP3 native virions baculovirus coexpression of genes. cis-acting signal involved localized within the 26 3'-terminal nucleotides reporter RNA....
We previously reported that the expression of rotavirus phenotypes by reassortants was affected recipient genetic background and proposed specific interactions between outer capsid proteins VP4 VP7 as basis for phenotypic effects (D. Chen, J. W. Burns, M. K. Estes, R. F. Ramig, Proc. Natl. Acad. Sci. USA 86:3743-3747, 1989). A neutralizing, cross-reactive VP4-specific monoclonal antibody (MAb), 2G4, used to probe protein-protein interactions. The specificity 2G4 confirmed immunoblot...