A5043326039- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Electrowetting and Microfluidic Technologies
- Nanofabrication and Lithography Techniques
- 3D Printing in Biomedical Research
- Additive Manufacturing and 3D Printing Technologies
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Pluripotent Stem Cells Research
- Electrospun Nanofibers in Biomedical Applications
- Advanced Biosensing Techniques and Applications
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Analytical Chemistry and Sensors
Hahn-Schickard-Gesellschaft für angewandte Forschung
2017-2021
University of Freiburg
2010-2017
BioFluidix (Germany)
2016
Abstract In tissue engineering applications, vascularization can be accomplished by coimplantation of forming cells and endothelial (ECs), whereby the latter are able to form functional blood vessels. The use three‐dimensional (3D) bioprinting technologies has potential improve classical approach because these will allow generation scaffolds with high spatial control cell allocation. This study focuses on a side comparison popular commercially available hydrogels (Matrigel, fibrin, collagen,...
We present (1) a fast and automated method for large scale production of HUVEC spheroids based on the hanging drop (2) novel well-controlled lateral deposition single by drop-on-demand printing. Large spheroid is achieved via printing 1536 droplets cell suspension having volume 1 μl each within 3 min at pitch 2.3 mm an array 48 × 32 onto flat substrate. Printing efficiencies between 97.9% 100% plating 87.3% were achieved. Harvested (consisting approx. 250 HUVECs each) appear uniform in size...
We demonstrate the use of photosensitive epoxy laminate TMMF S2045 for fabrication and sealing tapered microfluidic channels. The 45 µm thick resist enables shallow sealed cavities featuring extreme aspect ratios less than 1:40 (h = µm, w 2000 µm). It also provides high resolution minimum feature sizes 10 µm. For free-standing structures, an ratio up to 7:1 was achieved. dry-film photoresist can be applied easily by lamination onto structured substrates. total thickness variation across a...
Abstract Mesenchymal stem cells (MSCs) represent a very attractive cell source for tissue engineering applications aiming at the generation of artificial bone substitutes. The use three‐dimensional bioprinting technologies has potential to improve classical approach because will allow hydrogel scaffolds with high spatial control MSC allocation within bioprinted construct. In this study, we have performed direct comparisons between commercially available hydrogels in context their...
We investigate TMMF photopolymer as a cost-efficient alternative to glass for the leak-tight sealing of high-density silicon microchannels. enables low temperature and access structures underneath via lamination standard UV-lithography instead costly machining anodic bonding. is highly transparent has autofluorescence wavelengths larger than 400 nm. As too thin implementing bulky world-to-chip interfaces, we propose adhesive bonding cyclic olefin copolymer (COC) modules. All materials were...
Gel electrophoresis is one of the most applied and standardized tools for separation analysis macromolecules their fragments in academic research industry. In this work we present a novel approach conducting on‐demand electrophoretic separations DNA molecules open microfluidic (OM) systems on planar polymer substrates. The combines advantages slab gel, capillary‐ chip‐based methods offering low consumable costs (<0.1$) circumventing cost‐intensive chip fabrication, short process times (5...
Microfluidic systems fabricated in polydimethylsiloxane (PDMS) enable a broad variety of applications and are widespread the field Lab-on-a-Chip. Here we demonstrate semi-contact-writing, novel method for fabrication polymer based molds casting microfluidic PDMS chips highly flexible, time cost-efficient manner. The is related to direct-writing an aqueous solution on planar glass substrate substitutes conventional, time- cost-consuming UV-lithography. This technique facilitates on-demand...
Protein electrophoresis and immunoblotting are indispensable analytical tools for the characterization of proteins posttranslational modifications in complex sample matrices. Owing to lack automation, commonly employed slab-gel systems suffer from high time demand, significant sample/antibody consumption, limited reproducibility. To overcome these limitations, we developed a paper-based open microfluidic platform electrophoretic protein separation subsequent transfer protein-binding...
This paper describes a process sequence for the fabrication of all-polymer microfluidic chips based on multilayer lamination TMMF dry resist (TOK, Japan) pre-patterned PMMA substrate. The provides simple way to meet major requirements like embedded microchannels, nozzles and interconnecting vias as well their accurate integration into chip-interface without additional materials bonding procedures. We demonstrate applicability by manufacturing testing 24-channel TopSpot printhead....
Polyacrylamide usually is the material of choice for electrophoretic separation in slab gels, capillaries, and microfluidic devices. So far its polymerization requires anaerobic environments because oxygen impurities inhibit or even terminate reaction acrylamide. Here, it demonstrated that gel precursor solutions with glycerol contents above 20 vol% enable direct atmospheric photopolymerization acrylamide no need sealing degassing solution advance. The positive effect on proven by...
We report a new approach to perform on-demand electrophoretic separation of DNA. In contrast standard chip-based capillary electrophoresis in micromachined glass chips, we apply planar polyimide substrate, write 200 μm wide gel lines bridging two Pt-electrodes and inject 500 pl sample volumes non-contact manner. The is covered with mineral oil inhibit evaporation. Subsequently, an electrical field applied for 80 s the DNA molecules (56 bp-Cy5 112 bp-Cy5, 10 μM) successfully demonstrated.