A5089008753- 3D Printing in Biomedical Research
- Additive Manufacturing and 3D Printing Technologies
- Pluripotent Stem Cells Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Glycosylation and Glycoproteins Research
- Lipid Membrane Structure and Behavior
- Renal and related cancers
- Carbohydrate Chemistry and Synthesis
- Organ Donation and Transplantation
- Chronic Kidney Disease and Diabetes
- Renal cell carcinoma treatment
- Electrospun Nanofibers in Biomedical Applications
- Cellular Mechanics and Interactions
- Genetic and Kidney Cyst Diseases
- Cancer Cells and Metastasis
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Bone Tissue Engineering Materials
- RNA Interference and Gene Delivery
- Bacteriophages and microbial interactions
University of Freiburg
2013-2023
University of Zurich
2023
Abstract In tissue engineering applications, vascularization can be accomplished by coimplantation of forming cells and endothelial (ECs), whereby the latter are able to form functional blood vessels. The use three‐dimensional (3D) bioprinting technologies has potential improve classical approach because these will allow generation scaffolds with high spatial control cell allocation. This study focuses on a side comparison popular commercially available hydrogels (Matrigel, fibrin, collagen,...
The generation of artificial human tissue by 3D-bioprinting has expanded significantly as a clinically relevant research topic in recent years. However, to produce complex and viable tissue, in-depth biological understanding advanced printing techniques are required with high number process parameters. Here, we systematically evaluate the parameters for hybrid bioprinting based on fused-deposition modeling (FDM) thermoplastic material microextrusion cell-laden hydrogel. First, investigated...
Abstract Lectins are glycan‐binding proteins that involved in the recognition of glycoconjugates at cell surface. When binding to glycolipids, multivalent lectins can affect their distribution and alter membrane shapes. Neolectins have now been designed with controlled number position sites decipher role multivalency on avidity a glycosylated surface dynamics glycolipids. A monomeric hexavalent neolectin has first engineered from trimeric bacterial lectin, From this template, 13 different...
Abstract Scalable fabrication concepts of 3D kidney tissue models are required to enable their application in pharmaceutical high-throughput screenings. Yet the reconstruction complex structures remains technologically challenging. We present a novel concept reducing demands, by using controlled cellular self-assembly achieve higher complexities from significantly simplified construct designs. used drop-on-demand bioprinting fabricate locally confined patterns renal epithelial cells embedded...
Multivalency is proposed to play a role in the strong avidity of lectins for glycosylated cell surfaces and also their ability affect membrane dynamics by clustering glycosphingolipids. Lectins with modified valency were designed from β-propeller fold Ralstonia solanacearum lectin (RSL) that presents six fucose binding sites. After identification key amino acids molecular calculations, two mutants reduced produced. Isothermal titration calorimetry confirmed loss three high affinity sites...
Abstract Mesenchymal stem cells (MSCs) represent a very attractive cell source for tissue engineering applications aiming at the generation of artificial bone substitutes. The use three‐dimensional bioprinting technologies has potential to improve classical approach because will allow hydrogel scaffolds with high spatial control MSC allocation within bioprinted construct. In this study, we have performed direct comparisons between commercially available hydrogels in context their...
Bioprinting can be considered as a progression of the classical tissue engineering approach, in which cells are randomly seeded into scaffolds. offers advantage that placed with high spatial fidelity within three-dimensional constructs. A decisive factor to addressed for bioprinting approaches artificial tissues is almost all human body depend on functioning vascular system supply oxygen and nutrients. In this study, we have generated cuboid prevascularized bone constructs by adipose-derived...
Spheroids, organoids, or cell-laden droplets are often used as building blocks for bioprinting, but so far little is known about the spatio-temporal cellular interactions subsequent to printing. We a drop-on-demand bioprinting approach study biological of such in dimensions micrometers. Highly-density (approximately 700 cells 10 nL) multiple cell types were patterned 3D hydrogel matrix with precision up 70 μm. The patterns investigate endothelial (HUVECs) and adipose-derived mesenchymal stem...
Active nutrient supply and waste product removal are key requirements for the fabrication of long-term viable functional tissue constructs considerable size. This work aims to contribute artificial perfusable networks with a bioprinting process, based on drop-on-demand (DoD) printing primary endothelial cell (EC) suspension bioink (25 × 106 ± 3 cells/ml). The process results in prescribed lumen between two hydrogel layers, allowing its integration common layering processes. Low volume...
We used arrays of bioprinted renal epithelial cell spheroids for toxicity testing with cisplatin. The concentration-dependent death rate was determined using a lactate dehydrogenase assay. Bioprinted showed enhanced sensitivity to the treatment in comparison monolayers same type. measured dose-response curves revealed an inhibitory concentration IC50 = 9 ± 3 μM contrast 17 2 μM. Fluorescent labeling nephrotoxicity biomarker, kidney injury molecule 1 indicated accumulation central lumen...
Renal tubular cells frequently lose differentiation markers and physiological properties when propagated in conventional cell culture conditions. Embedding 3D microenvironments or controlling their assembly by bioprinting can enhance properties, which is beneficial for modeling diseases vitro. A potential cellular source renal physiology kidney vitro are directly reprogrammed induced epithelial (iRECs). iRECs were cultured various biomaterials as bioprinted structures. They showed high...
Abstract Lectins are glycan‐binding proteins that involved in the recognition of glycoconjugates at cell surface. When binding to glycolipids, multivalent lectins can affect their distribution and alter membrane shapes. Neolectins have now been designed with controlled number position sites decipher role multivalency on avidity a glycosylated surface dynamics glycolipids. A monomeric hexavalent neolectin has first engineered from trimeric bacterial lectin, From this template, 13 different...
The generation of artificial human tissue by 3D-bioprinting has expanded significantly as a clinically relevant research topic in recent years. However, to produce complex and viable tissue, in-depth biological understanding advanced printing techniques are required with high number process parameters. Here, we systematically evaluate the parameters for hybrid bioprinting based on fused-deposition modeling (FDM) thermoplastic material microextrusion cell-laden hydrogel. First, investigated...
We present a novel combination of drop on demand (DoD) and extrusion-based bioprinting to generate high-precision patterns cells inside large hydrogel volumes. Extrusion-based has the great advantage enabling fast deposition high viscous cell-loaded with reasonable precision. Compromises between shape fidelity cell viability, as well short process times often require many iterations optimizing parameters varying compositions hydrogel. To limit multitude during bioprinting, method for rapid...