A5011206251- 3D Printing in Biomedical Research
- Additive Manufacturing and 3D Printing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Pluripotent Stem Cells Research
- Renal and related cancers
- Cancer Cells and Metastasis
- Advanced Sensor and Energy Harvesting Materials
- Microfluidic and Capillary Electrophoresis Applications
- Bone Tissue Engineering Materials
- Cellular Mechanics and Interactions
- Mass Spectrometry Techniques and Applications
- Mesenchymal stem cell research
- Analytical Chemistry and Sensors
- Atomic and Subatomic Physics Research
- Sympathectomy and Hyperhidrosis Treatments
- Religious Education and Schools
- Biosensors and Analytical Detection
- Advanced MEMS and NEMS Technologies
- Neurological Disorders and Treatments
- Quantum, superfluid, helium dynamics
- Biomedical and Chemical Research
- Advanced Chemical Physics Studies
- Genetic and Kidney Cyst Diseases
- Animal and Plant Science Education
- Nanomaterials and Printing Technologies
University of Freiburg
2016-2024
Brigham and Women's Hospital
2024
Harvard University
2024
University of Regensburg
1989
LVR-Klinik Köln
1928
The generation of artificial human tissue by 3D-bioprinting has expanded significantly as a clinically relevant research topic in recent years. However, to produce complex and viable tissue, in-depth biological understanding advanced printing techniques are required with high number process parameters. Here, we systematically evaluate the parameters for hybrid bioprinting based on fused-deposition modeling (FDM) thermoplastic material microextrusion cell-laden hydrogel. First, investigated...
Abstract Scalable fabrication concepts of 3D kidney tissue models are required to enable their application in pharmaceutical high-throughput screenings. Yet the reconstruction complex structures remains technologically challenging. We present a novel concept reducing demands, by using controlled cellular self-assembly achieve higher complexities from significantly simplified construct designs. used drop-on-demand bioprinting fabricate locally confined patterns renal epithelial cells embedded...
Bioprinting can be considered as a progression of the classical tissue engineering approach, in which cells are randomly seeded into scaffolds. offers advantage that placed with high spatial fidelity within three-dimensional constructs. A decisive factor to addressed for bioprinting approaches artificial tissues is almost all human body depend on functioning vascular system supply oxygen and nutrients. In this study, we have generated cuboid prevascularized bone constructs by adipose-derived...
Spheroids, organoids, or cell-laden droplets are often used as building blocks for bioprinting, but so far little is known about the spatio-temporal cellular interactions subsequent to printing. We a drop-on-demand bioprinting approach study biological of such in dimensions micrometers. Highly-density (approximately 700 cells 10 nL) multiple cell types were patterned 3D hydrogel matrix with precision up 70 μm. The patterns investigate endothelial (HUVECs) and adipose-derived mesenchymal stem...
Abstract Droplet‐based bioprinting has shown remarkable potential in tissue engineering and regenerative medicine. However, it requires bioinks with low viscosities, which makes challenging to create complex 3D structures spatially pattern them different materials. This study introduces a novel approach sophisticated volumetric objects by merging droplet‐based cryobioprinting techniques. By leveraging the benefits of cryopreservation, we fabricated, for first time, intricate, self‐supporting...
Active nutrient supply and waste product removal are key requirements for the fabrication of long-term viable functional tissue constructs considerable size. This work aims to contribute artificial perfusable networks with a bioprinting process, based on drop-on-demand (DoD) printing primary endothelial cell (EC) suspension bioink (25 × 106 ± 3 cells/ml). The process results in prescribed lumen between two hydrogel layers, allowing its integration common layering processes. Low volume...
Introduction: Three-dimensional bioprinting can be considered as an advancement of the classical tissue engineering concept. For bioprinting, cells have to dispersed in hydrogels. Recently, a novel semi-synthetic thiolene hydrogel system based on norbornene-functionalized gelatin (GelNB) and thiolated (GelS) was described that resulted photoclick GelNB/GelS. In this study, we evaluated printability biocompatibility towards adipose-tissue-derived mesenchymal stem (ASCs). Methods: GelNB/GelS...
We used arrays of bioprinted renal epithelial cell spheroids for toxicity testing with cisplatin. The concentration-dependent death rate was determined using a lactate dehydrogenase assay. Bioprinted showed enhanced sensitivity to the treatment in comparison monolayers same type. measured dose-response curves revealed an inhibitory concentration IC50 = 9 ± 3 μM contrast 17 2 μM. Fluorescent labeling nephrotoxicity biomarker, kidney injury molecule 1 indicated accumulation central lumen...
Renal tubular cells frequently lose differentiation markers and physiological properties when propagated in conventional cell culture conditions. Embedding 3D microenvironments or controlling their assembly by bioprinting can enhance properties, which is beneficial for modeling diseases vitro. A potential cellular source renal physiology kidney vitro are directly reprogrammed induced epithelial (iRECs). iRECs were cultured various biomaterials as bioprinted structures. They showed high...
The development of highly conductive copper patterns on low-cost flexible substrates (PET, PEN, etc.) by inkjet printing is reported. Copper films were obtained from a metallo-organic decomposition (MOD) ink composed complex and suitable low-viscosity solvents. Upon heating the decomposed was converted into metallic under nitrogen as inert atmosphere.Additionally samples prepared using technology various substrates. required layer thickness for current conduction assessed PET sintering at...
Drug Prescribing for Patients with Chronic Kidney Disease in General Practice: a Cross-Sectional Study
For the first time, we present a flow sensor based on differential pressure principle consisting of exchangeable low-cost consumable parts, to minimize risk cross-contamination in medical applications, for example. In our case, component consists two thin-walled elastic silicone tubes connected by rigid capillary that can be exchanged easily and costeffectively after use. We characterized current prototype range from 0 up 50 μL/s water aqueous glycerin solutions with glycerol concentrations...
The generation of artificial human tissue by 3D-bioprinting has expanded significantly as a clinically relevant research topic in recent years. However, to produce complex and viable tissue, in-depth biological understanding advanced printing techniques are required with high number process parameters. Here, we systematically evaluate the parameters for hybrid bioprinting based on fused-deposition modeling (FDM) thermoplastic material microextrusion cell-laden hydrogel. First, investigated...
Printed conductors have attracted strong interest in academia as well the industry. While first applications using printed on flat curved surfaces are establishing market, extensive research still is conducted post-processing technologies needed for high-volume fabrication of solution processed conductors.With regards to potential low-cost, high-throughput manufacturing inexpensive polymeric foils, new start evolve that call an even more elaborate investigation printing and steps included....
We present a novel combination of drop on demand (DoD) and extrusion-based bioprinting to generate high-precision patterns cells inside large hydrogel volumes. Extrusion-based has the great advantage enabling fast deposition high viscous cell-loaded with reasonable precision. Compromises between shape fidelity cell viability, as well short process times often require many iterations optimizing parameters varying compositions hydrogel. To limit multitude during bioprinting, method for rapid...
Abstract Spheroids, organoids, or highly-dense cell-laden droplets are often used as building blocks for bioprinting, but so far little is known about the spatio-temporal cellular interactions post printing. We present a drop-on-demand approach to study biological of such in micrometer dimensions. Droplets (containing approximately 700 cells 10 nl) multiple cell types patterned 3D hydrogel matrix with precision less than 70 μm. It applied investigate relevant vascularization approaches. show...