A5082299315- Parasite Biology and Host Interactions
- Parasites and Host Interactions
- Helminth infection and control
- Parasitic Diseases Research and Treatment
- Parasitic infections in humans and animals
- Congenital Anomalies and Fetal Surgery
- Insects and Parasite Interactions
- Coccidia and coccidiosis research
- Parasitic Infections and Diagnostics
- Leech Biology and Applications
- Bird parasitology and diseases
- Nematode management and characterization studies
- Escherichia coli research studies
- Protist diversity and phylogeny
- Aquaculture disease management and microbiota
- Amoebic Infections and Treatments
- Marine Ecology and Invasive Species
- Microbial infections and disease research
- Legionella and Acanthamoeba research
- Date Palm Research Studies
- Forensic Entomology and Diptera Studies
- Myxozoan Parasites in Aquatic Species
- Phenothiazines and Benzothiazines Synthesis and Activities
- Identification and Quantification in Food
- Venomous Animal Envenomation and Studies
Instituto de Salud Carlos III
2016-2025
Centro Nacional de Microbiologia
2015-2025
Centro de Investigación Biomédica en Red
2024
Universidad Complutense de Madrid
1996-2022
Centro Nacional de Epidemiología
2019-2020
Centre for Biomedical Network Research on Rare Diseases
2018
Abstract Objectives To improve the diagnosis of human fascioliasis caused by Fasciola hepatica and gigantica , we evaluated diagnostic accuracy an enzyme‐linked immunosorbent assay (ELISA), with antigen from adult liver fluke, for detection IgG against in sera. Methods The sera 54 cases, originating three endemic areas, were used this evaluation: (i) a hyperendemic F. area where humans usually shed great number parasite eggs faeces (11 sera); (ii) epidemic small amounts (24 sera) (iii)...
Background Human fasciolosis is a re-emerging disease worldwide and caused by species of the genus Fasciola (F. hepatica F. gigantica). can be diagnosed classical coprological techniques, such as Kato-Katz test, to reveal parasite eggs in faeces. However, although 100% specific, these methods are generally not adequate for detection acute infections, ectopic or infections with low number parasites. In cases immunological may good alternative recommended use major hospitals where trained...
The risk of infection with Anisakis has been recognized for some time, but it is now emerging due to major awareness, better diagnostic techniques, and increasing preference raw or lightly cooked food. Spain the second-highest reported incidence after Japan, though real anisakidosis burden unknown because scarcity epidemiological data. This study provides a 19-year review anisakidosis-related hospitalizations describing trends patient characteristics.
Abstract Different agencies have emphasized the need to evaluate current serological methods for screening patients with suspected urogenital schistosomiasis. However, there is still a lack of evidence regarding most appropriate this purpose. Here we assessed diagnostic efficacy newly developed technique that utilizes recombinant protein Sh -TSP-2, applied urine and serum migrants having The sensitivity, specificity, positive negative predictive values an in-house enzyme-linked immunosorbent...
This study aimed to determine the cause of acute recidivous urticaria in patients who usually eat fish or other seafood. Twenty-five were studied. The skin prick test with larval Anisakis simplex extract was performed; total and specific IgE against A. measured CAP System; antibodies determined by ELISA; immunorecognition patterns sera studied Western blot. Nineteen showed simplex, but Ascaris demonstrated only two patients. No reacted Toxocara canis Echinoccocus granulosus antigens same...
Blood-feeding parasites have developed biochemical mechanisms to control heme intake and detoxification. Here we show that a major antigen secreted by Fasciola hepatica, previously reported as MF6p, of unknown function (gb|CCA61804.1), FhHDM-1, considered be helminth defense molecule belonging the family cathelicidin-like proteins (gb|ADZ24001.1), is in fact heme-binding protein. The nature MF6p/FhHDM-1 protein was revealed two independent experiments: (i) immunopurification protein·heme...
Eukaryotic cells respond to DNA damage by activating checkpoint pathways, which arrest cell cycle progression and induce gene expression. We isolated a full-length cDNA encoding 49-kDa protein from Leishmania major, exhibited significant deduced amino acid sequence homology with the annotated sp. damage-inducible (Ddi1-like) protein, as well Ddi1 Saccharomyces cerevisiae. In contrast previously described L. major displays three domains: (1) an NH2-terminal ubiquitin like; (2) COOH terminal...
Although Lloviu virus (LLOV) was discovered in the carcasses of insectivorous Schreiber’s Bent-winged bats caves Northern Spain 2002, its infectivity and pathogenicity remain unclear. We examined seroprevalence LLOV potentially exposed (n = 60), common serotine 10) as controls, humans 22) using an immunoblot assay. found antibodies against GP2 all serum pools, but not any human pools tested. To confirm this seroreactivity, 52 serums were individually tested Domain Programmable Arrays (DPA),...
Onchocerciasis or "river blindness" is a chronic parasitic neglected tropical disease which endemic both in mainland and insular Equatorial Guinea. We aim to estimate the current epidemiological situation of onchocerciasis Bioko Island after vector elimination 2005 more than sixteen years Community Directed Treatment with Ivermectin (CDTI) by using molecular serological approaches for diagnosis. A community-based cross-sectional study was carried out from mid-January mid-February 2014. total...
Currently, the reference standard assay for serodiagnosis of neurocysticercosis (NCC) is lentil lectin-bound glycoproteins/enzyme-linked immunoelectrotransfer blot (LLGP-EITB). The main disadvantage this technique complexity obtaining and purifying LLGP extract. This could be solved by replacement with highly specific recombinant antigens from Taenia solium. Based on previous studies, we selected produced Ts8B2 T24H proteins applied them to three diagnostic techniques: western (WB),...
MF6p/FhHDM-1 is a small cationic heme-binding protein which recognized by the monoclonal antibody (mAb) MF6, and abundantly present in parenchymal cells secreted antigens of Fasciola hepatica. Orthologs this (MF6p/HDMs) also exist other causal agents important foodborne trematodiasis, such as Clonorchis sinensis, Opisthorchis viverrini Paragonimus westermani. Considering that relevant for heme homeostasis was reported to have immunomodulatory properties, expected be useful target...
Background Onchocerciasis, also known as river blindness, is a parasitic disease. More than 99 percent of all cases occur in Africa. Bioko Island (Equatorial Guinea) the only island endemic for onchocerciasis world. Since 2005, when vector Simulium yahense was eliminated, there have not been any reported infection. This study aimed to demonstrate that updated WHO criteria stopping mass drug administration (MDA) met. Methodology/Principal findings A cross-sectional conducted from September...
The results obtained in a study of seroprevalence by means ELISA and immunoblot with crude larval extracts Anisakis simplex using 1008 human sera from Spanish people showing no clinical suspicion anisakidosis are given. For the evaluation Diagnostic Index (DI) was used, as ratio between optical density resulting test serum negative control. Forty-seven showed DIs 1.5 2, 14 were greater than 2. After comparison analysis immunorecognition pattern reference serum, diagnostic criterion could be...
This paper reports a case of myiasis caused by Hypoderma sinense in European man returning from journey through northern India. The patient showed eosinophilia, systemic signs inflammation, and painful swellings several parts the body. diagnosis was confirmed specific serology parasite molecular identification.
Onchocerciasis or “river blindness” is a chronic parasitic disease caused by the filarial worm Onchocerca volvulus, transmitted through infected blackflies (Simulium spp.). Bioko Island (Equatorial Guinea) used to show high endemicity for onchocerciasis. During last years, control programmes using different larvicides and ivermectin administration have considerably reduced prevalence intensity of infection. Based on this new epidemiological scenario, in present work we aimed assess impact...
A murine experimental model of anisakiasis has been developed in BALB/c and C57BL/10 mice orally inoculated with an Anisakis simplex living third stage larva (L3) order to investigate isotype-specific immune responses against excretory-secretory (ES) products crude extracts (CE) from L3. Specific antibody production showed similar patterns both ES CE antigens higher levels the latter. The dynamics earliest BALB/c, which antibodies were principally immunoglobulin (Ig)M isotype. Responses IgG1...