Since 1960, a total of seven species monkey malaria have been reported as transmissible to man by mosquito bite: Plasmodium cynomolgi, brasilianum, eylesi, knowlesi, inui, schwetzi and simium. With the exception P. none other has found infect humans in nature. In this report, it is described first known case naturally acquired cynomolgi humans. The patient was 39-year-old woman from malaria-free area with no previous history or travel endemic areas. Initially, diagnosed identified...

Abstract Previously, Plasmodium knowlesi was not considered as a species of that could cause malaria in human beings, it is parasite long-tailed ( Macaca fascicularis ) and pig-tailed nemestrina macaques found Southeast Asia. A case infection by P. described Spanish traveller, who came back to Spain with daily fever after his last overseas travel, which six-month holiday forested areas Asia between 2008 2009. His detected multiplex Real time quantitative PCR confirmed sequencing the...

We present filaria-nested polymerase chain reaction (PCR), which is based on amplification of first internal transcribed spacer rDNA to distinguish three parasitic filarial species (Onchocerca volvulus, Mansonella ozzardiand perstans) that can be found in the Amazon Region. Nested PCR-based identifications yielded same results as those utilizing morphological characters. PCR highly sensitive and specific it detects low-level infections both humans vectors. No cross-amplifications were...

Schistosomiasis and soil-transmitted helminthiases (STH) are two parasitic diseases mainly affecting school children. The purpose of this study was to estimate the current prevalence infection intensity, in addition associations these infections with age sex, children aged 4-17 years living Osun State, Nigeria. From each participant (250 children), one urine stool sample were taken for study, microscopic detection eggs or larvae faeces by means Kato-Katz method filtrated urine. overall...

Abstract Background Nigeria accounts for the greatest burden of malaria disease globally. Malaria control requires an effective treatment after diagnosis. The efficacy antimalarial drugs can be assessed through analysis genetic changes associated with reduced drug sensitivity. Methods This study includes markers artemisinin ( pfk13 ), sulfadoxine–pyrimethamine pfdhfr and pfdhps chloroquine its derivatives pfmdr1 pfcrt ) resistances, in blood samples collected from asymptomatic children...

To develop a new technique for diagnosis of Plasmodium knowlesi and at the same time to be able discriminate among diverse species causing human malaria. In this study nested multiplex malaria PCR was redesigned, targeting 18S rRNA gene, identify fifth species, knowlesi, together with other (Plasmodium falciparum, vivax, ovale malariae) by amplified fragment size using only two amplification processes including an internal reaction control avoid false negatives. The validated 91 clinical...

Abstract Background Strongyloides stercoralis is an intestinal parasite that can cause chronic infection, hyperinfection and/or a dissemination syndrome in humans. The use of techniques targeting ova fails to detect S. , as only larvae the are excreted faeces. Due absence “Gold” standard diagnostic method for there paucity reported data worldwide. Objective This study aimed evaluate performance methods infection by taking composite reference standard. Methods A cross-sectional was conducted...

The Kingdom of Saudi Arabia is seeking malaria eradication. Malaria transmission has been very low over the last few years. Discovered cases Plasmodium falciparum infection are assigned a treatment protocol artemisinin-based combination therapy, which consists artesunate in addition to sulfadoxine-pyrimethamine rather than traditional chloroquine, high resistance rates worldwide. This study aims investigate presence different gene mutations concerning anti-malarial drug (pfdhfr, pfdhps,...

Onchocerciasis or "river blindness" is a chronic parasitic neglected tropical disease which endemic both in mainland and insular Equatorial Guinea. We aim to estimate the current epidemiological situation of onchocerciasis Bioko Island after vector elimination 2005 more than sixteen years Community Directed Treatment with Ivermectin (CDTI) by using molecular serological approaches for diagnosis. A community-based cross-sectional study was carried out from mid-January mid-February 2014. total...

Loa loa and Mansonella perstans are two very common filarial species in Africa. Although microscopy is the traditional diagnostic method for human filariasis, several polymerase chain reaction (PCR) methods have emerged as an alternative approach identifying parasites. The aim of this study to compare three molecular decide which most suitable diagnosing loiasis mansonellosis non-endemic regions using dried blood spot (DBS) a medium sample collection storage.A total 100 DBS samples, with...

Background Strongyloides stercoralis is a parasite that causes strongyloidiasis in humans. It prevalent the tropics and sub-tropics where poor sanitation common problem. The true prevalence of S . Ethiopia underestimated due to lack “Gold” standard diagnostic method. Moreover, its across altitudinal gradient Amhara Region has not been studied. Methods A cross-sectional study was conducted among 844 schoolchildren from April December 2019. stool sample collected each participant processed...

Mansonellosis is endemic in several regions of Africa, the Caribbean, and Latin America. Mansonella ozzardi perstans have been reported America, including Amazon region. A morphological molecular microfilariae study was performed Pauini (Brazil). Blood samples were collected from 40 individuals, analyzed by Giemsa-stained blood film two different nested polymerase chain reactions which detect internal transcribed spacer-1 major sperm protein gene. By microscopy, 14 positive: 11 as M. three...

<title>Abstract</title> Background Nigeria has a high prevalence of malaria, being asymptomatic patients one the possible reservoirs malaria. In that context, accurate diagnosis is essential for malaria control. Rapid Diagnostic Test (RDT) efficacy threatened by false negatives due to<italic>pfhrp2</italic>and<italic>pfhrp3</italic>deletions. This study aims to describe in school children Osun State, southwestern and assess performance diagnosis, including characterization...

Despite the worldwide distribution of Coxiella burnetii, Q fever is not a frequent infection in travelers. This disease should be considered recent travelers from tropics with unknown fever, headache, raised liver enzymes, and low platelets. Five cases western to returning are presented.

Malaria is one of the deadliest diseases in world, particularly Africa. As such, resistance to anti-malarial drugs most important problems terms global malaria control. This study assesses evolution different markers over time and possible influence interventions treatment changes that have been made Equatorial Guinea.A total 1223 biological samples obtained period 1999 2019 were included study. Screening for mutations pfdhfr, pfdhps, pfmdr1, pfcrt genes was carried out by nested PCR...

Loa loa is a filarial species found exclusively in West and Central Africa. Microscopy the traditional diagnosis method for human loiasis. Several molecular methods have developed as an alternative approach identification of L. parasites.The aim this study was to evaluate Loa-Loop-mediated isothermal amplification (LAMP) assay diagnose loiasis disease on dried blood spots (DBS) samples, compared microscopy, filaria-real time-polymerase chain reaction (PCR) nested-Loa PCR.A total 100 DBS...

Loiasis, caused by the filarial nematode Loa loa, is endemic in Central and West Africa. loa has been associated with severe adverse reactions high Loa-infected individuals receiving ivermectin during mass drug administration programs for control of onchocerciasis lymphatic filariasis. Diagnosis loiasis still depends on microscopy blood samples, but this not effective large-scale surveys. New diagnostics methods are urgently needed. Previously, we developed a colorimetric high-sensitive...

Infections with the filarial nematodes Loa loa and Mansonella perstans are among most neglected infections. L. is endemic in 11 countries of Central West Africa loiasis estimated to affect about 20 million people. M. infection widespread more than 30 sub-Saharan Africa. Due difficulty diagnosing mansonellosis on a clinical basis, diagnosis relies laboratory techniques. Definitive based detection, identification, quantification circulating microfilariae (mf) by microscopy concentrated blood....

ABSTRACT. Malaria control and elimination require prompt diagnosis accurate treatment. Conventional methods such as rapid diagnostic tests (RDTs) microscopy lack the characteristics to detect low parasitemias, commonly found in asymptomatic parasitemias and/or submicroscopic malaria carriers. On contrary, molecular have higher sensitivity specificity. This study evaluated performance of two commercial real-time polymerase chain reaction (PCR) assays, RealStar ® PCR (RealStar-genus)...