A5000402570- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Parasitic Infections and Diagnostics
- Helminth infection and control
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Amoebic Infections and Treatments
- COVID-19 Clinical Research Studies
- Insect symbiosis and bacterial influences
- Diagnosis and treatment of tuberculosis
- Respiratory viral infections research
- COVID-19 diagnosis using AI
- Viral gastroenteritis research and epidemiology
- Pneumonia and Respiratory Infections
- Gut microbiota and health
- Child Nutrition and Water Access
- Viral Infections and Vectors
- Parasitic infections in humans and animals
- Biosensors and Analytical Detection
- Research on Leishmaniasis Studies
- Ocular Surface and Contact Lens
- Molecular Biology Techniques and Applications
- Connexins and lens biology
- Trypanosoma species research and implications
- Iron Metabolism and Disorders
Ospedale Sacro Cuore Don Calabria
2016-2025
University of Cambridge
2020-2023
Istituti di Ricovero e Cura a Carattere Scientifico
2018-2023
Montefiore Hospital
2015
University of Oxford
2014
Memorial
1987
Significance Members of the 2-oxoglutarate (2OG)-dependent oxygenase superfamily catalyze a range important biological oxidations. Structurally informed bioinformatic predictions suggest that human genome encodes as yet unassigned members superfamily. We describe work demonstrating 2OG and Fe(II)-dependent domain-containing protein 1 (OGFOD1) is hydroxylase modifies small ribosomal subunit RPS23 at conserved prolyl residue in ribosome-decoding center suppression or deletion OGFOD1 associated...
Schistosomiasis is a neglected infection affecting millions of people, mostly living in sub-Saharan Africa. Morbidity and mortality due to chronic are relevant, although schistosomiasis often clinically silent. Different diagnostic tests have been implemented order improve screening diagnosis, that traditionally rely on parasitological with low sensitivity. Aim this study was evaluate the accuracy different for African migrants, non endemic setting.A retrospective conducted 373 patients...
Strongyloidiasis is a neglected tropical disease caused by the human infective nematodes Strongyloides stercoralis, fuelleborni and kellyi. Previous large-scale studies exploring genetic diversity of this important genus have focused on Southeast Asia, with small number isolates from USA, Switzerland, Australia several African countries having been genotyped. Consequently, little known about global distribution geographic sub-variants these that exists within generally. We extracted DNA...
Public health measures for COVID-19 mitigation influenced the circulation of Respiratory Syncytial Virus (RSV) during 2020-2021 winter season. In following autumn, an unprecedented resurgence RSV occurred. Our study monitored pediatric infections one and two years after relaxation containment pandemic.
Data from recent studies support the hypothesis that infections by human gastrointestinal (GI) helminths impact, directly and/or indirectly, on composition of host gut microbial flora. However, to best our knowledge, these have been conducted in helminth-endemic areas with multi-helminth volunteers underlying disorders. Therefore, this study, we explore impact natural mono-infections parasite Strongyloides stercoralis faecal microbiota and metabolic profiles a cohort non-endemic area...
SUMMARY Strongyloides stercoralis is a parasite that can cause death in immunocompromised people. A proper diagnosis hence essential. The real-time polymerase-chain reaction (RT–PCR) novel, promising diagnostic method, detects the DNA of stool samples. In this retrospective study, we compared sensitivity agar plate coproculture (APC), an in-house immunofluorescence test (IFAT) and RT–PCR for S. infection. study sample was composed by 223 Samples resulting positive to APC, IFAT were 20, 140...
Background: We assessed the sensitivity, specificity and positive negative predictive value (PPV NPV) of molecular serological tests for diagnosis SARS-CoV-2 infection. Methods: A total 346 patients were enrolled in emergency room. evaluated three Reverse Transcriptase-real time PCRs (RT-PCRs) including six different gene targets, five serologic rapid diagnostic (RDT) one ELISA. The final classification infected/non-infected was performed using Latent Class Analysis combined with clinical...
In Italy, the epidemiology of tick-borne pathogens is still poorly characterized. This prospective study was conducted at IRCCS Sacro Cuore Don Calabria Hospital in Negrar di Valpolicella (Verona), northeastern from 2018 to 2022. Ticks asymptomatic individuals visiting hospital after a recent tick bite were characterized using microscopy and tested for molecular tests. A total 317 ticks collected 280 subjects analyzed, with most identified as Ixodes species (95.6%), followed by Rhipicephalus...
In this study we characterized the presence and subtype (ST1-ST4) of Blastocystis in patients attended at a referral center for tropical diseases Northern Italy. We also, evaluated organism's association with other intestinal parasites. Parasite screening was performed on 756 patients, from different geographical origins (namely, Italians, Africans, South Americans, Asian non-Italian Europeans) which Italians represented largest group. seen to be most prevalent parasite study. Subtype 3 1...
The SARS-CoV-2 virus spread in the Northern Hemisphere during 2019/2020 influenza seasons and it persisted 2021/2022 season. A cocirculation of viruses was expected Italy winter seasons. This study aims to investigate prevalence respiratory syncytial observed a hospital Verona Province, these past three compare our data with national global surveillance reports on transmission preceding decade. Our findings clearly demonstrated extremely low among hospitalized patients outpatients first two...
With the continuous spread of new SARS-CoV-2 variants concern (VOCs), monitoring diagnostic test performances is mandatory. We evaluated changes in antigen tests’ (ADTs) accuracy along Delta to Omicron VOCs transition, exploring N protein mutations possibly affecting ADT sensitivity and assessing best sampling site for diagnosis infections. In total, 5175 subjects were enrolled from 1 October 2021 15 July 2022. The inclusion criteria combined with a same-day RT-PCR swab test. For analysis,...
Strongyloides stercoralis is a soil-transmitted helminth with wide distribution in tropical and subtropical areas. The diagnosis of S. stercoralisinfection can be challenging, due to the low sensitivity microscopic examination stool samples coproculture. In last decade, different in-house molecular biology techniques for have been implemented. They demonstrated good accuracy, although does not seem sufficiently high yet. Recently, novel PCR technique has evaluated detection DNA urine. Aim...
ObjectivesIn Italy the burden of patients with coronavirus disease 2019 (COVID-19) gradually decreased from March to end May. In this work we aimed evaluate a possible association between severity clinical manifestations and viral load over time during epidemiological transition high-to low-transmission settings.MethodsWe reviewed cases COVID-19 diagnosed at emergency room our hospital, retrieving proportion admitted intensive care unit. A raw estimation was done evaluating Ct (cycle...
Microscopic examination of stool samples has been considered to be the "gold standard" for diagnosis intestinal parasites. Recently, polymerase chain reaction (PCR) approved by World Health Organization as method choice Entamoeba histolytica. Of 106 collected from Esmeraldas and Pichincha provinces Ecuador, all (100%) were positive E. histolytica/Entamoeba dispar light microscopy, whereas using real-time PCR (RT-PCR) DNA amplification, 74 (69.8%) only three (2.8%) Some 29 (27.4%) negative...
While symbiotic relationships between invertebrates and bacteria have been extensively described, studies of microbial communities inhabiting parasitic worms remain scarce. Exploring the microbiota associated with helminths responsible for major infectious diseases will inform on parasite biology, host-pathogen interactions, disease pathophysiology. We investigated presence microorganisms tissues human Schistosoma mansoni. In situ hybridization using a pan-bacterial 16S rRNA gene probe...
Background: Dried blood spots (DBS) are used for epidemiological surveys on infectious diseases in settings where limited resources available. In fact, DBS can help to overcome logistic difficulties the collection, transport and storage of biological specimens. Objective: To evaluate accuracy Strongyloides stercoralis serology performed DBS. Methods: A survey was proposed children attending a school village Borbon, Ecuador, their parents/guardians. Each participant gave consent collection...
The delay between onset of symptoms and coronary care unit admission is decisive in the outcome patients with acute myocardial infarction.To evaluate influence factors that affect infarction treatment.Multicenter case-control study conducted by 118 units Italy. median mean times cases controls were compared for decision time, home-to-hospital in-hospital several potential risk on was evaluated comparison admitted more than 6 hours after those within onset.Among 5301 infarction, 590 who came...
Aim: To obtain the first molecular epidemiological survey of Tropheryma whipplei intestinal colonization in Italy. Materials & methods: Retrospective, observational study to assess prevalence T. whipplei, causative agent Whipple's disease, stool samples (real-time PCR) patients attending Center for Tropical Diseases (Italy) and risk factors associated. Results: Overall was 6.9% (85/1240). The younger age group showed a significantly higher rate than older (12.7 vs 5.9%, p = 0.002). 4.9%...
For many years microscopy has been considered the mainstay of diagnosis parasitic infections. In our laboratory, before advent molecular biology, approach for identification infections in stools was microscopic exam three samples. Once we adopted a real-time PCR on one single sample added to classical coproparasitological Given high sensitivity (Rt-PCR), then decided evaluate if change routine justified. detail, intended assess much more practical routine, based analysis fecal sample,...