A5053924318- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Helminth infection and control
- Parasitic Diseases Research and Treatment
- Viral Infections and Vectors
- Biosensors and Analytical Detection
- Vector-borne infectious diseases
- Mosquito-borne diseases and control
- Vector-Borne Animal Diseases
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 detection and testing
- Research on Leishmaniasis Studies
- Insect symbiosis and bacterial influences
- Malaria Research and Control
- Parasitic Infections and Diagnostics
- Coccidia and coccidiosis research
- Travel-related health issues
- Trypanosoma species research and implications
- Global Maternal and Child Health
- Insects and Parasite Interactions
- Drug-Induced Hepatotoxicity and Protection
- Aquaculture disease management and microbiota
- COVID-19 diagnosis using AI
- Rabies epidemiology and control
- Infectious Diseases and Mycology
Universidad de Salamanca
2016-2025
Instituto de Investigación Biomédica de Salamanca
2016-2025
Biomedical Research Institute
2019
Background Human schistosomiasis, mainly due to Schistosoma mansoni species, is one of the most prevalent parasitic diseases worldwide. To overcome drawbacks classical parasitological and serological methods in detecting S. infections, especially acute stage disease, development cost-effective, simple rapid molecular still needed for diagnosis schistosomiasis. A promising approach loop-mediated isothermal amplification (LAMP) technology. Compared PCR-based assays, LAMP has advantages...
Background In Brazil, schistosomiasis is a parasitic disease of public health relevance, mainly in poor areas where Schistosoma mansoni the only human species encountered and Biomphalaria straminea one intermediate host snails. A nested-PCR based on specific mitochondrial S. minisatellite DNA region has been successfully developed applied as reference method Brazil for detection, snails epidemiological studies. The amplification efficiency LAMP known to be higher than PCR. present work aimed...
Schistosomiasis is one of the most prevalent Neglected Tropical Disease, affecting approximately 250 million people worldwide. Schistosoma mansoni important species causing human intestinal schistosomiasis. Despite significant efforts in recent decades, global disease burden schistosomiasis remains extremely high. This could partly be attributed to absence accurate diagnostic tools, primarily endemic areas. Loop-mediated isothermal amplification (LAMP) increasingly used molecular diagnostics...
The SARS-CoV-2 pandemic has forced all countries worldwide to rapidly develop and implement widespread testing control manage the Coronavirus Disease 2019 (COVID-19). reverse-transcription (RT)-qPCR is gold standard molecular diagnostic method for COVID-19, mostly in automated platforms. These systems are accurate effective, but also costly, time-consuming, high-technological, infrastructure-dependent, currently suffer from commercial reagent supply shortages. loop-mediated isothermal...
Nucleic acid amplification diagnostics offer outstanding features of sensitivity and specificity. However, they still lack speed robustness, require extensive infrastructure, are neither affordable nor user-friendly. Thus, have not been extensively applied in point-of-care diagnostics, particularly low-resource settings. In this work, we combined the loop-mediated isothermal (LAMP) technology with a handheld portable device (SMART-LAMP) developed to perform real-time nucleic reactions, based...
Urogenital schistosomiasis due to Schistosoma haematobium is a serious underestimated public health problem affecting 112 million people - particularly in sub-Saharan Africa. Microscopic examination of urine samples detect parasite eggs still remains as definitive diagnosis. This work was focussed on developing novel loop-mediated isothermal amplification (LAMP) assay for detection S. DNA human high-throughput, simple, accurate and affordable diagnostic tool use diagnosis urogenital...
Malaria is the parasitic disease with highest morbidity and mortality worldwide. Angola one of five sub-Saharan African countries malaria burden. Real-time PCR diagnosis in endemic areas has not been implemented due to its high cost need for adequate infrastructure. Dried blood spots (DBSs) are an alternative collecting, preserving, transporting samples reference laboratories. The objective study was assess efficacy DBS as a sampling method research studies employing real-time PCR. divided...
The filarial parasite Loa loa, the causative agent of loiasis, is endemic in Central and Western Africa infecting 3–13 million people. L. loa has been associated with fatal encephalopathic reactions high Loa-infected individuals receiving ivermectin during mass drug administration programs for control onchocerciasis lymphatic filariasis. In areas, only diagnostic method routinely used microscopic examination mid-day blood samples by thick film. Improved methods detection are needed regions...
Loop-mediated isothermal amplification (LAMP) is the most popular technology for point-of-care testing applications due its rapid, sensitive and specific detection with simple instrumentation compared to PCR-based methods. Many systems reading results of LAMP amplifications exist, including real-time fluorescence using fluorophore-labelled probes attached oligonucleotide sequences complementary target nucleic acid. This methodology allows simultaneous multiple targets (multiplexing) in one...
Fasciolosis is considered the most widespread trematode disease affecting grazing animals around world; it currently recognised by World Health Organisation as an emergent human pathogen. Triclabendazole still effective drug against this disease; however, resistant strains have appeared and developing vaccine has increasingly become a priority. Several bioinformatics tools were here used for predicting B- T-cell epitopes according to available data Fasciola hepatica protein amino acid...
Background Strongyloides stercoralis, the chief causative agent of human strongyloidiasis, is a nematode globally distributed but mainly endemic in tropical and subtropical regions. Chronic infection often clinically asymptomatic it can result severe hyperinfection syndrome or disseminated strongyloidiasis immunocompromised patients. There great diversity techniques used diagnosing disease, definitive diagnosis accomplished by parasitological examination stool samples for morphological...
Background Schistosomiasis remains one of the most common endemic parasitic diseases affecting over 230 million people worlwide. Schistosoma mansoni is main species causing intestinal and hepatic schistosomiasis fresh water pulmonate snails genus Biomphalaria are best known for their role as intermediate hosts parasite. The development new molecular monitoring assays large-scale screening from transmission sites to detect presence schistosomes an important point consider snail control...
Crimean-Congo haemorrhagic fever (CCHF) is a potentially lethal tick-borne viral disease with wide distribution. In Spain, 12 human cases of CCHF have been confirmed, four deaths. The diagnosis hampered by the nonspecific symptoms, high genetic diversity CCHFV, and biosafety requirements to manage virus. RT-qPCR serological tests are used for limitations. Reverse-transcription loop-mediated isothermal amplification (RT-LAMP) could be an effective alternative in disease. However, none few...
Schistosomiasis is a water-borne disease afflicting over 261 million people in many areas of the developing countries with high morbidity and mortality. The control relies mainly on treatment praziquantel. Fatty acid binding proteins (FABP) have demonstrated levels immune-protection against trematode infections. This study reports immunoprotection induced by cross-reacting Fasciola hepatica FABP, native (nFh12) recombinantly expressed using two different expression systems Escherichia coli...
Schistosomiasis is a significant public health problem in sub-Saharan Africa, China, South-East Asia and regions of South central America affecting about 189 million people. Kunitz-type serine protease inhibitors have been identified as important players the interaction other flatworm parasites with their mammalian hosts. Here, we evaluate protective efficacy chemically synthesized T- B-cell peptide epitopes derived from kunitz protein Schistosoma mansoni. Putative kunitz-type inhibitor...
Background Schistosoma mansoni is the main species causing hepatic and intestinal schistosomiasis in Sub-Saharan Africa, it only South America. Adult stages of parasite reside mesenteric venous plexus infected hosts, eggs are shed feces. Collecting patient stool samples for S. diagnostic purposes difficult large-scale field trials. Urine would be an alternative approach molecular detection since they have several advantages over samples, including better handling, management storage....