A5009984491- Parasites and Host Interactions
- Helminth infection and control
- Parasite Biology and Host Interactions
- Research on Leishmaniasis Studies
- Coccidia and coccidiosis research
- Higher Education Teaching and Evaluation
- Parasitic Diseases Research and Treatment
- Educational Technology in Learning
- E-Learning and Knowledge Management
- Macrophage Migration Inhibitory Factor
- Trypanosoma species research and implications
- Health and Medical Education
- Parasitic infections in humans and animals
- vaccines and immunoinformatics approaches
- Vector-borne infectious diseases
- Animal health and immunology
- Parasitic Infections and Diagnostics
- Trace Elements in Health
- Vector-Borne Animal Diseases
- Insect symbiosis and bacterial influences
- Aquaculture disease management and microbiota
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Drug-Induced Hepatotoxicity and Protection
- Bird parasitology and diseases
Universidad de Salamanca
2016-2025
Instituto de Investigación Biomédica de Salamanca
2016-2025
Consejo Superior de Investigaciones Científicas
1978-1997
Instituto de Recursos Naturales y Agrobiología de Salamanca
1997
Background Human schistosomiasis, mainly due to Schistosoma mansoni species, is one of the most prevalent parasitic diseases worldwide. To overcome drawbacks classical parasitological and serological methods in detecting S. infections, especially acute stage disease, development cost-effective, simple rapid molecular still needed for diagnosis schistosomiasis. A promising approach loop-mediated isothermal amplification (LAMP) technology. Compared PCR-based assays, LAMP has advantages...
Currently available methods for the diagnosis of human schistosomiasis often lack enough sensitivity and specificity. Recently, several authors have developed more specific sensitive diagnostic methods, mainly based on polymerase chain reaction (PCR) technique. Nevertheless, these been only applied 1 out 4 Schistosoma species affecting man (S. mansoni). Additionally, application PCR has exclusively used blood or faecal patients' samples. Here, we develop a new, high approach that allows...
Schistosomiasis is one of the most prevalent Neglected Tropical Disease, affecting approximately 250 million people worldwide. Schistosoma mansoni important species causing human intestinal schistosomiasis. Despite significant efforts in recent decades, global disease burden schistosomiasis remains extremely high. This could partly be attributed to absence accurate diagnostic tools, primarily endemic areas. Loop-mediated isothermal amplification (LAMP) increasingly used molecular diagnostics...
Introduction Schistosomiasis, one of the current Neglected Tropical Diseases (NTDs) affects over 230 million people globally, with nearly 700 at risk in more than 74 countries. Praziquantel (PZQ) has served as primary treatment for past four decades; however, its effectiveness is limited it solely eliminates adult worms. In regions where infections are frequent, PZQ exhibits only temporary efficacy and restricted potential to disrupt prolonged transmission disease.
Urogenital schistosomiasis due to Schistosoma haematobium is a serious underestimated public health problem affecting 112 million people - particularly in sub-Saharan Africa. Microscopic examination of urine samples detect parasite eggs still remains as definitive diagnosis. This work was focussed on developing novel loop-mediated isothermal amplification (LAMP) assay for detection S. DNA human high-throughput, simple, accurate and affordable diagnostic tool use diagnosis urogenital...
Fasciola hepatica is a parasitic trematode responsible for fascioliasis, significant zoonotic disease affecting livestock worldwide, as well humans. This study identifies peptides with potential use in vaccines against and validates multi-epitope constructs from those vitro. Putative protein sequences derived the genome of F. were integrated phase-specific transcriptomic data to prioritize highly expressed proteins. Among these, extracellular proteins selected using DeepLoc 2.0 strong...
Background The leishmaniases are a complex of neglected tropical diseases caused by more than 20 Leishmania parasite species, for which available therapeutic arsenal is scarce and unsatisfactory. Pentavalent antimonials (SbV) currently the first-line pharmacologic therapy leishmaniasis worldwide, but resistance to these compounds increasingly reported. Alkyl-lysophospoholipid analogs (ALPs) constitute family with antileishmanial activity, one its members, miltefosine, has been approved as...
The filarial parasite Loa loa, the causative agent of loiasis, is endemic in Central and Western Africa infecting 3–13 million people. L. loa has been associated with fatal encephalopathic reactions high Loa-infected individuals receiving ivermectin during mass drug administration programs for control onchocerciasis lymphatic filariasis. In areas, only diagnostic method routinely used microscopic examination mid-day blood samples by thick film. Improved methods detection are needed regions...
Background Schistosomiasis is the third most devastating tropical disease worldwide caused by blood flukes of genus Schistosoma. This parasitic due to immunologic reactions Schistosoma eggs trapped in tissues. Egg-released antigens stimulate tissue-destructive inflammatory and granulomatous reactions, involving different immune cell populations, including T cells granulocytes. Granulomas lead collagen fibers deposition fibrosis, resulting organ damage. Praziquantel (PZQ) drug choice for...
Fasciolosis is considered the most widespread trematode disease affecting grazing animals around world; it currently recognised by World Health Organisation as an emergent human pathogen. Triclabendazole still effective drug against this disease; however, resistant strains have appeared and developing vaccine has increasingly become a priority. Several bioinformatics tools were here used for predicting B- T-cell epitopes according to available data Fasciola hepatica protein amino acid...
Context C-6-Geranylated flavonoids possess promising biological activities. These substances could be a source of lead compounds for the development therapeutics. Objective The study was designed to evaluate their antibacterial and antileishmanial activity. Materials methods flavanones were tested in micromolar concentrations against promastigote forms Leishmania brazilensis, L. donovani, infantum, panamensis methicillin-resistant Staphylococcus aureus (MRSA); synergistic potential with...
Background Strongyloides stercoralis, the chief causative agent of human strongyloidiasis, is a nematode globally distributed but mainly endemic in tropical and subtropical regions. Chronic infection often clinically asymptomatic it can result severe hyperinfection syndrome or disseminated strongyloidiasis immunocompromised patients. There great diversity techniques used diagnosing disease, definitive diagnosis accomplished by parasitological examination stool samples for morphological...
Background Schistosomiasis remains one of the most common endemic parasitic diseases affecting over 230 million people worlwide. Schistosoma mansoni is main species causing intestinal and hepatic schistosomiasis fresh water pulmonate snails genus Biomphalaria are best known for their role as intermediate hosts parasite. The development new molecular monitoring assays large-scale screening from transmission sites to detect presence schistosomes an important point consider snail control...
Background Schistosomiasis is a parasitic disease caused by trematodes of the genus Schistosoma. Five species Schistosoma are known to infect humans, out which S. haematobium most prevalent, causing chronic schistosomiasis that still represents major problem public health in many regions world and especially tropical areas, leading serious manifestations mortality developing countries. Since 1970s, praziquantel (PZQ) drug choice for treatment schistosomiasis, but concerns about relying on...
Schistosomiasis is a water-borne disease afflicting over 261 million people in many areas of the developing countries with high morbidity and mortality. The control relies mainly on treatment praziquantel. Fatty acid binding proteins (FABP) have demonstrated levels immune-protection against trematode infections. This study reports immunoprotection induced by cross-reacting Fasciola hepatica FABP, native (nFh12) recombinantly expressed using two different expression systems Escherichia coli...