A5048085944- Malaria Research and Control
- vaccines and immunoinformatics approaches
- Mosquito-borne diseases and control
- Tuberculosis Research and Epidemiology
- RNA and protein synthesis mechanisms
- Mycobacterium research and diagnosis
- HIV Research and Treatment
- Complement system in diseases
- Parasites and Host Interactions
- Invertebrate Immune Response Mechanisms
- Lipid Membrane Structure and Behavior
- Monoclonal and Polyclonal Antibodies Research
- Pneumonia and Respiratory Infections
- Immunotherapy and Immune Responses
- Drug Transport and Resistance Mechanisms
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- interferon and immune responses
- Herpesvirus Infections and Treatments
- Computational Drug Discovery Methods
- Hepatitis B Virus Studies
- Insect Resistance and Genetics
- Parasite Biology and Host Interactions
- Mass Spectrometry Techniques and Applications
- Antimicrobial Peptides and Activities
Fundación Instituto de Inmunología de Colombia
2012-2022
Universidad del Rosario
2008-2018
Universidad Nacional de Colombia
2000-2014
Universidad de Antioquia
2013
Universidad Colegio Mayor de Cundinamarca
2012
Corporación para Investigaciones Biológicas
2000
The mycobacterial cell envelope has been implicated in the pathogenicity of tuberculosis and therefore a prime target for identification characterization surface proteins with potential application drug vaccine development. In this study, genome Mycobacterium H37Rv was screened using Machine Learning tools that included feature-based predictors, general localizers transmembrane topology predictors to identify are potentially secreted M. tuberculosis, or extracellular milieu through different...
Schistosomiasis is a significant public health problem in sub-Saharan Africa, China, South-East Asia and regions of South central America affecting about 189 million people. Kunitz-type serine protease inhibitors have been identified as important players the interaction other flatworm parasites with their mammalian hosts. Here, we evaluate protective efficacy chemically synthesized T- B-cell peptide epitopes derived from kunitz protein Schistosoma mansoni. Putative kunitz-type inhibitor...
Determining immune protection-inducing protein structures (IMPIPS) involves defining the stereo-electron and topochemical characteristics which are essential in MHC-p-TCR complex formation. Modified high activity binding peptides (mHABP) were thus synthesised to produce a large panel of IMPIPS measuring 26.5 ±3.5Å between farthest atoms fitting into Pockets 1 9 HLA-DRβ1* structures. They displayed polyproline II-like (PPIIL) structure with their backbone O N orientated establish H-bonds...
To date, the function of many hypothetical membrane proteins Mycobacterium tuberculosis is still unknown and their involvement in pathogen-host interactions has not been yet clearly defined. In this study, biological activity peptides derived from protein Rv0679c M. was assessed. Transcription gene studied 26 spp. Strains. Antibodies raised against putative B-cell epitopes were used Western blot immunoelectron microscopy assays. Synthetic spanning entire length tested for ability to bind...
The merozoite surface protein‐1 represents a prime candidate for development of malaria vaccine. Merozoite has been shown to demonstrate high‐activity peptide binding human red blood cells. One the peptides, named 5501, located in N‐terminus (amino acid sequence MLNISQHQCVKKQCPQNS) 19‐kDa molecular mass fragment protein‐1, is conserved, nonimmunogenic and nonprotective. Its critical residues were identified replaced with amino acids similar but different charge, order modify their...
The goal of this study was to demonstrate the usefulness an enzyme-linked immunosorbent assay (ELISA) for serodiagnosis pulmonary tuberculosis (PTB) and extrapulmonary TB (EPTB). This used 20 amino acid-long, non-overlapped synthetic peptides that spanned complete Mycobacterium ESAT-6 Ag85A sequences. validation cohort consisted 1,102 individuals who were grouped into following five diagnostic groups: 455 patients with PTB, 60 EPTB, 40 non-EPTB, 33 leprosy 514 healthy controls. For PTB...
The gene encoding the Mycobacterium tuberculosis Rv2536 protein is present in complex (as assayed by PCR) and transcribed determined RT-PCR) M. H37Rv, H37Ra, bovis BCG, africanum strains. Rabbits immunized with synthetic polymer peptides from this produced antibodies specifically recognizing a 25-kDa band mycobacterial sonicate. U937 A549 cells were used binding assays involving 20-amino-acid-long covering whole sequence. Peptide 11207 (161DVFSAVRADDSPTGEMQVAQY180) presented high specific to...
Given the urgent need for designing a new antituberculosis vaccine conferring total protection on patients of all ages, following line research adopted by our institute, this work has identified Mycobacterium tuberculosis (Mtb) Rv3166c protein high-activity binding peptides (HABPs) which are able to inhibit bacterial invasion U937 (monocyte-derived macrophages) and A549 (type II alveolar epithelial cells) cell lines. The presence transcription rv3166c gene in Mtb species complex was...
The Rv2707 gene encoding a putative alanine- and leucine-rich protein was found to be present in all Mycobacterium tuberculosis complex strains (by PCR) its transcription shown by RT-PCR but M. bovis microti. Antibodies raised against peptides specifically recognized the native Western blot were able locate this on membrane immunoelectron microscopy. A549 U937 cells lines used binding assays involving synthetic covering whole protein. High cell-binding peptide 16083 (281...