A5086120713- Genetics, Aging, and Longevity in Model Organisms
- Developmental Biology and Gene Regulation
- CRISPR and Genetic Engineering
- Neurobiology and Insect Physiology Research
- Epigenetics and DNA Methylation
- Chromosomal and Genetic Variations
- Molecular Biology Techniques and Applications
- Lung Cancer Treatments and Mutations
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cancer Genomics and Diagnostics
- Hippo pathway signaling and YAP/TAZ
University of North Carolina at Chapel Hill
2012-2018
UNC Lineberger Comprehensive Cancer Center
2018
University of Southern Mississippi
2013
A large collection of new modENCODE and ENCODE genome-wide chromatin data sets from cell lines developmental stages in worm, fly human are analysed; this reveals many conserved features organization among the three organisms, as well notable differences composition locations repressive chromatin. This study describes numerous Homo sapiens, Drosophila melanogaster Caenorhabditis elegans generated by consortia. The results point to while identifying Genome function is dynamically regulated...
RNA polymerase transcription initiation sites are largely unknown in Caenorhabditis elegans . The initial 5′ end of most protein-coding transcripts is removed by trans -splicing, and noncoding have not been investigated. We characterized the landscape Pol II initiation, identifying 73,500 distinct clusters initiation. Bidirectional frequent, with a peak transcriptional pairing at 120 bp. assign to 7691 genes find that they display features typical eukaryotic promoters. Strikingly, majority...
The Caenorhabditis elegans dosage compensation complex (DCC) equalizes X-chromosome gene between XO males and XX hermaphrodites by two-fold repression of X-linked expression in hermaphrodites. DCC localizes to the X chromosomes but not males, some subunits form a homologous condensin. mechanism which downregulates remains unclear. Here we show that controls methylation state lysine 20 histone H4, leading higher H4K20me1 lower H4K20me3 levels on relative autosomes. We identify PR-SET7...
Abstract Serial monitoring of plasma DNA mutations in estrogen receptor positive metastatic breast cancer (ER + MBC) holds promise as an early predictor therapeutic response. Here, we developed dPCR-SEQ, a customized assay that utilizes digital PCR-based target enrichment followed by next-generation sequencing to analyze ESR1 , PIK3CA and TP53 . We validated dPCR-SEQ prospective cohort 58 patients with ER MBC demonstrate excellent concordance hotspot mutation abundance measured conventional...